LOCUS       sequence-306423-        8224 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8224)
  AUTHORS   Corbi AL, Kishimoto TK, Miller LJ, Springer TA
  TITLE     The human leukocyte adhesion glycoprotein Mac-1 (complement receptor
            type 3, CD11b) alpha subunit. Cloning, primary structure, and 
            relation to the integrins, von Willebrand factor and factor B.
  JOURNAL   J Biol Chem 1988 Sep 5;263(25):12403-11.
  PUBMED    2457584
REFERENCE   2  (bases 1 to 8224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem"; date: "1988-09-5"; volume: "263(25)"; pages: "12403-11"
COMMENT     Sequence Label: Human MAC-1 alpha
FEATURES             Location/Qualifiers
     source          1..8224
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        67..446
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        447..650
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     601..621
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     695..714
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        695..713
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          5128..5193
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5323..5343
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5615..5749
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5652..5671)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5706..5725
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6596..6731
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7226..7245
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      complement(7358..7816)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7495..7516)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7710..7729
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     tRNA            complement(join(7939..7958,7959..8043,8044..8118))
                     /product="E. coli amber-suppressor tyrosine tRNA"
                     /label=supF
ORIGIN
        1 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
       61 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
      121 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      181 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      241 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      301 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      361 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      421 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      481 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      541 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      601 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa
      661 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
      721 aagcttctag agatccctcg acctcgagat ccattgtgct ctaaagcatg gctctcagag
      781 tccttctgtt aacagccttg accttatgtc atgggttcaa cttggacact gaaaacgcaa
      841 tgaccttcca agagaacgca aggggcttcg ggcagagcgt ggtccagctt cagggatcca
      901 gggtggtggt tggagccccc caggagatag tggctgccaa ccaaaggggc agcctctacc
      961 agtgcgacta cagcacaggc tcatgcgagc ccatccgcct gcaggtcccc gtggaggccg
     1021 tgaacatgtc cctgggcctg tccctggcag ccaccaccag cccccctcag ctgctggcct
     1081 gtggtcccac cgtgcaccag acttgcagtg agaacacgta tgtgaaaggg ctctgcttcc
     1141 tgtttggatc caacctacgg cagcagcccc agaagttccc agaggccctc cgagggtgtc
     1201 ctcaagagga tagtgacatt gccttcttga ttgatggctc tggtagcatc atcccacatg
     1261 actttcggcg gatgaaggag tttgtctcaa ctgtgatgga gcaattaaaa aagtccaaaa
     1321 ccttgttctc tttgatgcag tactctgaag aattccggat tcactttacc ttcaaagagt
     1381 tccagaacaa ccctaaccca agatcactgg tgaagccaat aacgcagctg cttgggcgga
     1441 cacacacggc cacgggcatc cgcaaagtgg tacgagagct gtttaacatc accaacggag
     1501 cccgaaagaa tgcctttaag atcctagttg tcatcacgga tggagaaaag tttggcgatc
     1561 ccttgggata tgaggatgtc atccctgagg cagacagaga gggagtcatt cgctacgtca
     1621 ttggggtggg agatgccttc cgcagtgaga aatcccgcca agagcttaat accatcgcat
     1681 ccaagccgcc tcgtgatcac gtgttccagg tgaataactt tgaggctctg aagaccattc
     1741 agaaccagct tcgggagaag atctttgcga tcgagggtac tcagacagga agtagcagct
     1801 cctttgagca tgagatgtct caggaaggct tcagcgctgc catcacctct aatggcccct
     1861 tgctgagcac tgtggggagc tatgactggg ctggtggagt ctttctatat acatcaaagg
     1921 agaaaagcac cttcatcaac atgaccagag tggattcaga catgaatgat gcttacttgg
     1981 gttatgctgc cgccatcatc ttacggaacc gggtgcaaag cctggttctg ggggcacctc
     2041 gatatcagca catcggcctg gtagcgatgt tcaggcagaa cactggcatg tgggagtcca
     2101 acgctaatgt caagggcacc cagatcggcg cctacttcgg ggcctccctc tgctccgtgg
     2161 acgtggacag caacggcagc accgacctgg tcctcatcgg ggccccccat tactacgagc
     2221 agacccgagg gggccaggtg tccgtgtgcc ccttgcccag ggggagggct cggtggcagt
     2281 gtgatgctgt tctctacggg gagcagggcc aaccctgggg ccgctttggg gcagccctaa
     2341 cagtgctggg ggacgtaaat ggggacaagc tgacggacgt ggccattggg gccccaggag
     2401 aggaggacaa ccggggtgct gtttacctgt ttcacggaac ctcaggatct ggcatcagcc
     2461 cctcccatag ccagcggata gcaggctcca agctctctcc caggctccag tattttggtc
     2521 agtcactgag tgggggccag gacctcacaa tggatggact ggtagacctg actgtaggag
     2581 cccaggggca cgtgctgctg ctcaggtccc agccagtact gagagtcaag gcaatcatgg
     2641 agttcaatcc cagggaagtg gcaaggaatg tatttgagtg taatgatcag gtggtgaaag
     2701 gcaaggaagc cggagaggtc agagtctgcc tccatgtcca gaagagcaca cgggatcggc
     2761 taagagaagg acagatccag agtgttgtga cttatgacct ggctctggac tccggccgcc
     2821 cacattcccg cgccgtcttc aatgagacaa agaacagcac acgcagacag acacaggtct
     2881 tggggctgac ccagacttgt gagaccctga aactacagtt gccgaattgc atcgaggacc
     2941 cagtgagccc cattgtgctg cgcctgaact tctctctggt gggaacgcca ttgtctgctt
     3001 tcgggaacct ccggccagtg ctggcggagg atgctcagag actcttcaca gccttgtttc
     3061 cctttgagaa gaattgtggc aatgacaaca tctgccagga tgacctcagc atcaccttca
     3121 gtttcatgag cctggactgc ctcgtggtgg gtgggccccg ggagttcaac gtgacagtga
     3181 ctgtgagaaa tgatggtgag gactcctaca ggacacaggt caccttcttc ttcccgcttg
     3241 acctgtccta ccggaaggtg tccacactcc agaaccagcg ctcacagcga tcctggcgcc
     3301 tggcctgtga gtctgcctcc tccaccgaag tgtctggggc cttgaagagc accagctgca
     3361 gcataaacca ccccatcttc ccggaaaact cagaggtcac ctttaatatc acgtttgatg
     3421 tagactctaa ggcttccctt ggaaacaaac tgctcctcaa ggccaatgtg accagtgaga
     3481 acaacatgcc cagaaccaac aaaaccgaat tccaactgga gctgccggtg aaatatgctg
     3541 tctacatggt ggtcaccagc catggggtct ccactaaata tctcaacttc acggcctcag
     3601 agaataccag tcgggtcatg cagcatcaat atcaggtcag caacctgggg cagaggagcc
     3661 tccccatcag cctggtgttc ttggtgcccg tccggctgaa ccagactgtc atatgggacc
     3721 gcccccaggt caccttctcc gagaacctct cgagtacgtg ccacaccaag gagcgcttgc
     3781 cctctcactc cgactttctg gctgagcttc ggaaggcccc cgtggtgaac tgctccatcg
     3841 ctgtctgcca gagaatccag tgtgacatcc cgttctttgg catccaggaa gaattcaatg
     3901 ctaccctcaa aggcaacctc tcgtttgact ggtacatcaa gacctcgcat aaccacctcc
     3961 tgatcgtgag cacagctgag atcttgttta acgattccgt gttcaccctg ctgccgggac
     4021 agggggcgtt tgtgaggtcc cagacggaga ccaaagtgga gccgttcgag gtccccaacc
     4081 ccctgccgct catcgtgggc agctctgtcg ggggactgct gctcctggcc ctcatcaccg
     4141 ccgcgctgta caagctcggc ttcttcaagc ggcaatacaa ggacatgatg agtgaagggg
     4201 gtcccccggg ggccgaaccc cagtagcggc tccttcccga cagagctgcc tctcggtggc
     4261 cagcaggact ctgcccagac cacacgtagc ccccaggctg ctggacacgt cggacagcga
     4321 agtatccccg acaggacggg cttgggcttc catttgtgtg tgtgcaagtg tgtatgtgcg
     4381 tgtgtgcgag tgtgtgcaag tgtctgtgtg caagtgtgtg cacgtgtgcg tgtgcgtgca
     4441 tgtgcactcg cacgcccatg tgtgagtgtg tgcaagtatg tgagtgtgtc caagtgtgtg
     4501 tgcgtgtgtc catgtgtgtg caagtgtgtg catgtgtgcg agtgtgtgca tgtgtgtgct
     4561 caggggcgtg tggctcacgt gtgtgactca gatgtctctg gcgtgtgggt aggtgacggc
     4621 agcgtagcct ctccggcaga agggaactgc ctgggctccc ttgtgcgtgg gtgaagccgc
     4681 tgctgggttt tcctccggga gaggggacgg tcaatcctgt gggtgaagac agagggaaac
     4741 acagcagctt ctctccactg aaagaagtgg gacttcccgt cgcctgcgag cctgcggcct
     4801 gctggagcct gcgcagcttg gatggagact ccatgagaag ccgtgggtgg aaccaggagc
     4861 ctcctccaca ccagcgctga tgcccaataa agatgcccac tgaggaatga tgaaaaaaaa
     4921 aagaattcga gctcgcccgg ggatcctcta gagtcgacct gcagcccaag ctctttagag
     4981 cacaatggat ctcgagggat cttccatacc taccagttct gcgcctgcag gtcgcggccg
     5041 cgactctaga ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa
     5101 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt
     5161 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg
     5221 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct
     5281 agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag
     5341 gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt
     5401 agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta
     5461 tacaagaaaa ttatggaaaa atatttgatg tatagtgcct tgactagaga tcataatcag
     5521 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     5581 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     5641 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     5701 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc cgccatggta
     5761 tcaacgccat atttctattt acagtaggga cctcttcgtt gtgtaggtac cgctgtattc
     5821 ctagggaaat agtagaggca ccttgaactg tctgcatcag ccatatagcc cccgctgttc
     5881 gatttacaaa cacaggcaca gtactgacaa acccatacac ctcctctgaa atacccatag
     5941 ttgctagggc tgtctccgaa ctcattacac cctccaaagt cagagctgta atttcgccat
     6001 caagggcagc gagggcttct ccagataaaa tagcttctgc cgagagtccc gtaagggtag
     6061 acacttcagc taatccctcg atgaggtcta ctagaatagt cagtgcggct cccattttga
     6121 aaattcactt acttgatcag cttcagaaga tggcggaggg cctccaacac agtaattttc
     6181 ctcccgactc ttaaaataga aaatgtcaag tcagttaagc aggaagtgac taactgaccg
     6241 cagctggccg tgcgacatcc tcttttaatt agttgctagg caactgccct ccagagggca
     6301 gtgtggtttt gcaagaggaa gcaaaaagcc tctccaccca ggcctagaat gtttccaccc
     6361 aatcattact atgacaacag ctgttttttt tagtattaag cagaggccgg gggcccctgg
     6421 cctccgctta ctctggagaa aaagaagaga ggcattgtag aggcttccag aggcaacttg
     6481 tcaaaacagg actgcttcta tttctgtcac actgtctggc cctgtcacaa ggtccagcac
     6541 ctccataccc cctttaataa gcagtttggg aacgggtgcg ggtcttactc cgcccatccc
     6601 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     6661 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     6721 ttttggaggc ctaggctttt gcaaaaagct aattcggcgt aatctgctgc ttgcaaacaa
     6781 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6841 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     6901 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctgaagc
     6961 cagttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     7021 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7081 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     7141 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     7201 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     7261 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     7321 ggaaaaacgc cagcaacgca agctagcttc tagctagaaa ttgtaaacgt taatattttg
     7381 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     7441 ggcaaaatcc cttataaatc aaaagaatag cccgagatag ggttgagtgt tgttccagtt
     7501 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     7561 tatcagggcg atggccgccc actacgtgaa ccatcaccca aatcaagttt tttggggtcg
     7621 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     7681 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     7741 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     7801 ccgctacagg gcgcgtacta tggttgcttt gacgagcacg tataacgtgc tttcctcgtt
     7861 agaatcagag cgggagctaa acaggaggcc gattaaaggg attttagaca ggaacggtac
     7921 gccagctgga ccgcggtctt tcggactttt gaaagtgatg gtggtggggg aaggattcga
     7981 accttcgaag tcgatgacgg cagatttaga gtctgctccc tttggccgct cgggaacccc
     8041 accacaggta atgcttttac tggcctgctc ccttatcggg aagcggggcg catcatatca
     8101 aatgacgcgc cgctgtaaag tgttacgttg agaaagctgc tccctgcttg tgtgttggag
     8161 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     8221 tgca
//
