LOCUS       sequence-306425-       12448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac transposon vector with CAG promoter expressing T910M mutant
            SMARCA4 (FLAG-tagged) and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12448)
  AUTHORS   Ballabio C, Anderle M, Gianesello M, Lago C, Miele E, Cardano M, 
            Aiello G, Piazza S, Caron D, Gianno F, Ciolfi A, Pedace L, 
            Mastronuzzi A, Tartaglia M, Locatelli F, Ferretti E, Giangaspero F, 
            Tiberi L
  TITLE     Modeling medulloblastoma in vivo and with human cerebellar 
            organoids.
  JOURNAL   Nat Commun. 2020 Jan 29;11(1):583. doi: 10.1038/s41467-019-13989-3.
  PUBMED    31996670
REFERENCE   2  (bases 1 to 12448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-01-29"; volume: "11(1):583. doi"; pages: " 
            10.1038/s41467-019-13989-3"
COMMENT     Sequence Label: pPB CAG SMARCA4 T910M-IRES-EGFP
FEATURES             Location/Qualifiers
     source          1..12448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     621..640
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        621..639
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   706..768
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1112..1145
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        1173..1552
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1554..1831
                     /label=chicken beta-actin promoter
     intron          1832..2848
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2772..2794
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2856..2875
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             7746..7769
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(7810..8384,8385..8387,8388..8396)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7977..7994)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8204..8223
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(8397..8399,8400..8402,8403..9116)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8397..8399,8400..8402,8403..9113)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8442..8463)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8703..8722)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9050..9071
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(9208..9227)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9273..9328
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9327..9346)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    9675..9708
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     repeat_region   10075..10109
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(10258..10276)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10297..10313)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10297..10313)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10310..10332)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10321..10337
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10345..10351,10352..10369,10370..10375))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10390..10411
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10528..10545)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10699..11287)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10779..10798)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11458..12249,12250..12318))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12081..12100
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12319..12423)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgaattg taatacgact cactataggg cgaattggag ctcggtattc
      661 acgacagcag gctgaataat aaaaaaatta gaaactatta tttaacccta gaaagataat
      721 catattgtga cgtacgttaa agataatcat gcgtaaaatt gacgcatgtg ttttatcggt
      781 ctgtatatcg aggtttattt attaatttga atagatatta agttttatta tatttacact
      841 tacatactaa taataaattc aacaaacaat ttatttatgt ttatttattt attaaaaaaa
      901 aacaaaaact caaaatttct tctataaagt aacaaaactt ttaaacattc tctcttttac
      961 aaaaataaac ttattttgta ctttaaaaac agtcatgttg tattataaaa taagtaatta
     1021 gcttaaccta tacataatag aaacaaatta tacttattag tcagtcagaa acaactttgg
     1081 cacatatcaa tattatgctc tcgttaatcg cataacttcg tataatgtat gctatacgaa
     1141 gttataattc agatctttcg gcgcgccggg tcgacattga ttattgacta gttattaata
     1201 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     1261 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     1321 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     1381 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     1441 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     1501 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt
     1561 gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta
     1621 tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg gggggcgcgc
     1681 gccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggagag gtgcggcggc
     1741 agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg
     1801 gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc ttcgccccgt
     1861 gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc
     1921 acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg
     1981 acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt
     2041 gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg
     2101 cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct
     2161 ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg
     2221 cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg
     2281 cgcgtcggtc gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc
     2341 ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg
     2401 gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg
     2461 ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca
     2521 ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctgtgc
     2581 ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg cgaagcggtg
     2641 cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc
     2701 ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc gggggggacg
     2761 gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca
     2821 tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc
     2881 tcatcatttt ggcaaagaat tgcatgtcca ctccagaccc acccctgggc ggaactcctc
     2941 ggccaggtcc ttccccgggc cctggccctt cccctggagc catgctgggc cctagcccgg
     3001 gtccctcgcc gggctccgcc cacagcatga tggggcccag cccagggccg ccctcagcag
     3061 gacaccccat ccccacccag gggcctggag ggtaccctca ggacaacatg caccagatgc
     3121 acaagcccat ggagtccatg catgagaagg gcatgtcgga cgacccgcgc tacaaccaga
     3181 tgaaaggaat ggggatgcgg tcagggggcc atgctgggat ggggcccccg cccagcccca
     3241 tggaccagca ctcccaaggt tacccctcgc ccctgggtgg ctctgagcat gcctctagtc
     3301 cagttccagc cagtggcccg tcttcggggc cccagatgtc ttccgggcca ggaggtgccc
     3361 cgctggatgg tgctgacccc caggccttgg ggcagcagaa ccggggccca accccattta
     3421 accagaacca gctgcaccag ctcagagctc agatcatggc ctacaagatg ctggccaggg
     3481 ggcagcccct ccccgaccac ctgcagatgg cggtgcaggg caagcggccg atgcccggga
     3541 tgcagcagca gatgccaacg ctacctccac cctcggtgtc cgcaacagga cccggccctg
     3601 gccctggccc tggccccggc ccgggtcccg gcccggcacc tccaaattac agcaggcctc
     3661 atggtatggg agggcccaac atgcctcccc caggaccctc gggcgtgccc cccgggatgc
     3721 caggccagcc tcctggaggg cctcccaagc cctggcctga aggacccatg gcgaatgctg
     3781 ctgcccccac gagcacccct cagaagctga ttcccccgca gccaacgggc cgcccttccc
     3841 ccgcgccccc tgccgtccca cccgccgcct cgcccgtgat gccaccgcag acccagtccc
     3901 ccgggcagcc ggcccagccc gcgcccatgg tgccactgca ccagaagcag agccgcatca
     3961 cccccatcca gaagccgcgg ggcctcgacc ctgtggagat cctgcaggag cgcgagtaca
     4021 ggctgcaggc tcgcatcgca caccgaattc aggaacttga aaaccttccc gggtccctgg
     4081 ccggggattt gcgaaccaaa gcgaccattg agctcaaggc cctcaggctg ctgaacttcc
     4141 agaggcagct gcgccaggag gtggtggtgt gcatgcggag ggacacagcg ctggagacag
     4201 ccctcaatgc taaggcctac aagcgcagca agcgccagtc cctgcgcgag gcccgcatca
     4261 ctgagaagct ggagaagcag cagaagatcg agcaggagcg caagcgccgg cagaagcacc
     4321 aggaatacct caatagcatt ctccagcatg ccaaggattt caaggaatat cacagatccg
     4381 tcacaggcaa aatccagaag ctgaccaagg cagtggccac gtaccatgcc aacacggagc
     4441 gggagcagaa gaaagagaac gagcggatcg agaaggagcg catgcggagg ctcatggctg
     4501 aagatgagga ggggtaccgc aagctcatcg accagaagaa ggacaagcgc ctggcctacc
     4561 tcttgcagca gacagacgag tacgtggcta acctcacgga gctggtgcgg cagcacaagg
     4621 ctgcccaggt cgccaaggag aaaaagaaga aaaagaaaaa gaagaaggca gaaaatgcag
     4681 aaggacagac gcctgccatt gggccggatg gcgagcctct ggacgagacc agccagatga
     4741 gcgacctccc ggtgaaggtg atccacgtgg agagtgggaa gatcctcaca ggcacagatg
     4801 cccccaaagc cgggcagctg gaggcctggc tcgagatgaa cccggggtat gaagtagctc
     4861 cgaggtctga tagtgaagaa agtggctcag aagaagagga agaggaggag gaggaagagc
     4921 agccgcaggc agcacagcct cccaccctgc ccgtggagga gaagaagaag attccagatc
     4981 cagacagcga tgacgtctct gaggtggacg cgcggcacat cattgagaat gccaagcaag
     5041 atgtcgatga tgaatatggc gtgtcccagg cccttgcacg tggcctgcag tcctactatg
     5101 ccgtggccca tgctgtcact gagagagtgg acaagcagtc agcgcttatg gtcaatggtg
     5161 tcctcaaaca gtaccagatc aaaggtttgg agtggctggt gtccctgtac aacaacaacc
     5221 tgaacggcat cctggccgac gagatgggcc tggggaagac catccagacc atcgcgctca
     5281 tcacgtacct catggagcac aaacgcatca atgggccctt cctcatcatc gtgcctctct
     5341 caacgctgtc caactgggcg tacgagtttg acaagtgggc cccctccgtg gtgaaggtgt
     5401 cttacaaggg atccccagca gcaagacggg cctttgtccc ccagctccgg agtgggaagt
     5461 tcaacgtctt gctgacgacg tacgagtaca tcatcaaaga caagcacatc ctcgccaaga
     5521 tccgttggaa gtacatgatt gtggacgaag gtcaccgcat gaagaaccac cactgcaagc
     5581 tgacgcaggt gctcaacacg cactatgtgg caccccgccg cctgctgctg atgggcacac
     5641 cgctgcagaa caagcttccc gagctctggg cgctgctcaa cttcctgctg cccaccatct
     5701 tcaagagctg cagcaccttc gagcagtggt ttaacgcacc ctttgccatg accggggaaa
     5761 aggtggacct gaatgaggag gaaaccattc tcatcatccg gcgtctccac aaagtgctgc
     5821 ggcccttctt gctccgacga ctcaagaagg aagtcgaggc ccagttgccc gaaaaggtgg
     5881 agtacgtcat caagtgcgac atgtctgcgc tgcagcgagt gctctaccgc cacatgcagg
     5941 ccaagggcgt gctgctgact gatggctccg agaaggacaa gaagggcaaa ggcggcacca
     6001 agaccctgat gaacaccatc atgcagctgc ggaagatctg caaccacccc tacatgttcc
     6061 agcacatcga ggagtccttt tccgagcact tggggttcac tggcggcatt gtccaagggc
     6121 tggacctgta ccgagcctcg ggtaaatttg agcttcttga tagaattctt cccaaactcc
     6181 gagcaaccaa ccacaaagtg ctgctgttct gccaaatgac ctccctcatg accatcatgg
     6241 aagattactt tgcgtatcgc ggctttaaat acctcaggct tgatggaacc acgaaggcgg
     6301 aggaccgggg catgctgctg aaaaccttca acgagcccgg ctctgagtac ttcatcttcc
     6361 tgctcagcac ccgggctggg gggctcggcc tgaacctcca gtcggcagac actgtgatca
     6421 tttttgacag cgactggaat cctcaccagg acctgcaagc gcaggaccga gcccaccgca
     6481 tcgggcagca gaacgaggtg cgtgtgctcc gcctctgcac cgtcaacagc gtggaggaga
     6541 agatcctagc tgcagccaag tacaagctca acgtggacca gaaggtgatc caggccggca
     6601 tgttcgacca gaagtcctcc agccatgagc ggcgcgcctt cctgcaggcc atcctggagc
     6661 acgaggagca ggatgaggag gaagacaagg tgcccgacga cgagaccgtc aaccagatga
     6721 tcgcccggca cgaggaggag tttgatctgt tcatgcgcat ggacctggac cgcaggcgcg
     6781 aggaggcccg caaccccaag cggaagccgc gcctcatgga ggaggacgag ctcccctcgt
     6841 ggatcatcaa ggacgacgcg gaggtggagc ggctgacctg tgaggaggag gaggagaaga
     6901 tgttcggccg tggctcccgc caccgcaagg aggtggacta cagcgactca ctgacggaga
     6961 agcagtggct caaggccatc gaggagggca cgctggagga gatcgaagag gaggtccggc
     7021 agaagaaatc atcacggaag cgcaagcgag acagcgacgc cggctcctcc accccgacca
     7081 ccagcacccg cagccgcgac aaggacgacg agagcaagaa gcagaagaag cgcgggcggc
     7141 cgcctgccga gaaactctcc cctaacccac ccaacctcac caagaagatg aagaagattg
     7201 tggatgccgt gatcaagtac aaggacagca gcagtggacg tcagctcagc gaggtcttca
     7261 tccagctgcc ctcgcgaaag gagctgcccg agtactacga gctcatccgc aagcccgtgg
     7321 acttcaagaa gataaaggag cgcattcgca accacaagta ccgcagcctc aacgacctag
     7381 agaaggacgt catgctcctg tgccagaacg cacagacctt caacctggag ggctccctga
     7441 tctatgaaga ctccatcgtc ttgcagtcgg tcttcaccag cgtgcggcag aaaatcgaga
     7501 aggaggatga cagtgaaggc gaggagagtg aggaggagga agagggcgag gaggaaggct
     7561 ccgaatccga atctcggtcc gtcaaagtga agatcaagct tggccggaag gagaaggcac
     7621 aggaccagct gaagggcggc cggcggcggc cgagccgagg gtcccgagcc aagccggtcg
     7681 tgagtgacga tgacagtgag gaggaacaag aggaggaccg ctcaggaagt ggcagcgaag
     7741 aagacgacta caaggacgac gatgacaaat gaaattctgc agtcgacggt accgcgggcc
     7801 cgggatccgc ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata
     7861 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     7921 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     7981 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     8041 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     8101 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     8161 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     8221 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     8281 gcctcggtac acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     8341 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatgg
     8401 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     8461 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     8521 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     8581 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     8641 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     8701 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     8761 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     8821 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     8881 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     8941 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     9001 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     9061 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg
     9121 gccgcaattc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
     9181 tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat
     9241 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
     9301 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     9361 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc
     9421 catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
     9481 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
     9541 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
     9601 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga
     9661 cctgcaccgt cgagataact tcgtataatg tatgctatac gaagttatgc tagccaacaa
     9721 gctcgtcatc gctttgcaga agagcagaga ggatatgctc atcgtctaaa gaactaccca
     9781 ttttattata tattagtcac gatatctata acaagaaaat atatatataa taagttatca
     9841 cgtaagtaga acatgaaata acaatataat tatcgtatga gttaaatctt aaaagtcacg
     9901 taaaagataa tcatgcgtca ttttgactca cgcggtcgtt atagttcaaa atcagtgaca
     9961 cttaccgcat tgacaagcac gcctcacggg agctccaagc ggcgactgag atgtcctaaa
    10021 tgcacagcga cggattcgcg ctatttagaa agagagagca atatttcaag aatgcatgcg
    10081 tcaattttac gcagactatc tttctagggt taaaaaagat ttgcgcttta ctcgacctaa
    10141 actttaaaca cgtcatagaa tcttcgtttg acaaaaacca cattgtggcc aagctgtgtg
    10201 acgcgacgcg cgctaaagaa tggcaaacca agtcgcgcga ggtaccagct tttgttccct
    10261 ttagtgaggg ttaatttcga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
    10321 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
    10381 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
    10441 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
    10501 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
    10561 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
    10621 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
    10681 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    10741 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    10801 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    10861 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    10921 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    10981 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    11041 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    11101 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
    11161 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    11221 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    11281 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    11341 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    11401 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    11461 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    11521 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    11581 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    11641 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    11701 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    11761 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    11821 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    11881 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    11941 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    12001 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    12061 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    12121 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    12181 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    12241 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    12301 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    12361 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    12421 gcgcacattt ccccgaaaag tgccacct
//
