LOCUS       sequence-306426-       16287 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of PE2 enzyme under control of the Gal4-regulated UAS 
            promoter. Can be used to generate transgenic flies with white+ 
            selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16287)
  AUTHORS   Bosch JA, Birchak G, Perrimon N
  TITLE     Precise genome engineering in Drosophila using prime editing.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Jan 5;118(1). pii: 2021996118. doi: 
            10.1073/pnas.2021996118.
  PUBMED    33443210
REFERENCE   2  (bases 1 to 16287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-01-5"; volume: "118(1). pii: 
            2021996118. doi"; pages: " 10.1073/pnas.2021996118"
COMMENT     Sequence Label: pUAS-PE2-attB
FEATURES             Location/Qualifiers
     source          1..16287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(102..135)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    151..245
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    complement(258..291)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    309..739
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    977..1046
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(1169..1187)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1639..1710,2112..2385,2460..3114,3176..3491,3695..3826,
                     3897..4511)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     complement(5315..5334)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5316..5334)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5344..5361)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5344..5360)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5353..5375)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(5501..5957)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5639..5660)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5851..5870
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5984..6088
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6089..6157,6158..6949)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6307..6326)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7120..7708
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7609..7628
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7862..7879
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7996..8017
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8032..8037,8038..8055,8056..8062)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8070..8086
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8075..8097
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8094..8110
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8094..8110
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8129..8149
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8131..8149
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    8168..8598
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     polyA_signal    8620..8741
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8631..8650)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8685..8704
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(9013..9033)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          9163..9228
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     protein_bind    9535..9559
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(9579..9599)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(11772..13355,13356..13358,13359..15872))
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(11772..15872)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(15873..15893)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(15950..15974)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(16020..16258)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     complement(16052..16073)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    join(16282..16287,1..89)
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
ORIGIN
        1 ggacagtact ccgctcggag gacagtactc cgctcggagg acagtactcc gctcggagga
       61 cagtactccg ctcggaggac agtactccga cctgcggatc cataacttcg tatagcatac
      121 attatacgaa gttatccatg ggagtctccg ctcggaggac agtactccgc tcggaggaca
      181 gtactccgct cggaggacag tactccgctc ggaggacagt actccgctcg gaggacagta
      241 ctccgacctg cctgcagata acttcgtata gcatacatta tacgaagtta taagctggta
      301 ctactagtgt tgttggttgg cacaccacaa atatactgtt gccgagcaca attgatcggc
      361 taaatggtat ggcaagaaaa ggtatgcaat ataataatct tttattgggt atgcaacgaa
      421 aatttgtttc gtcaacgtat gcaatattct ttattaaaag agggtatgca atgtatttta
      481 ttaaaaacgg gtatgcaata taataatctt ttattgggta tgcaacgaaa atttgtttcg
      541 tcaaagtatg caatattttt tattaaaaga gggtatgcaa tgtattttat taaaaacggg
      601 tatgcaataa aaaattattt ggtttctcta aaaagtatgc agcacttatt ttttgataag
      661 gtatgcaaca aaattttact ttgccgaaaa tatgcaatgt ttttgcgaat aaattcaacg
      721 cacacttatt acgtggccaa ctagtgtacc gaattaggcc ttctagtgga tcaattcggc
      781 ttgtcgacat gcccgccgtg accgtcgaga acccgctgac gctgccccgc gtatccgcac
      841 cagccgacgc cgtcgcacgt cccgtgctca ccgtgaccac cgcgcccagc ggtttcgagg
      901 gcgagggctt cccggtgcgc cgcgcgttcg ccgggatcaa ctaccgccac ctcgacccgt
      961 tcatcatgat ggaccagatg ggtgaggtgg agtacgcgcc cggggagccc aagggcacgc
     1021 cctggcaccc gcaccgcggc ttcgagaccg tgacctacat cgtcgacggt acctgaagcc
     1081 gaattgatcc ggagagctcc caacgcgttg gatgcagcct cgagatcgat gatatcaagc
     1141 ttcttgcgat ctggcgcgcc tagtatgtat gtaagttaat aaaacccatt tttgcggaaa
     1201 gtagataaaa aaaacatttt ttttttttac tgcactggat atcattgaac ttatctgatc
     1261 agttttaaat ttacttcgat ccaagggtat ttgatgtacc aggttctttc gattacctct
     1321 cactcaaaat gacattccac tcaaagtcag cgctgtttgc ctccttctct gtccacagaa
     1381 atatcgccgt ctctttcgcc gctgcgtccg ctatctcttt cgccaccgtt tgtagcgtta
     1441 cgtagcgtca atgtccgcct tcagttgcat tttgtcagcg gtttcgtgac gaagctccaa
     1501 gcggtttacg ccatcaatta aacacaaagt gctgtgccaa aactcctctc gcttcttatt
     1561 tttgtttgtt ttttgagtga ttggggtggt gattggtttt gggtgggtaa gcaggggaaa
     1621 gtgtgaaaaa tcccggcaat gggccaagag gatcaggagc tattaattcg cggaggcagc
     1681 aaacacccat ctgccgagca tctgaacaat gtgagtagta catgtgcata catcttaagt
     1741 tcacttgatc tataggaact gcgattgcaa catcaaattg tctgcggcgt gagaactgcg
     1801 acccacaaaa atcccaaacc gcaattgcac aaacaaatag tgacacgaaa cagattattc
     1861 tggtagctgt tctcgctata taagacaatt tttgagatca tatcatgatc aagacatcta
     1921 aaggcattca ttttcgacta tattcttttt tacaaaaaat ataacaacca gatattttaa
     1981 gctgatccta gatgcacaaa aaataaataa aagtataaac ctacttcgta ggatacttcg
     2041 gggtactttt tgttcggggt tagatgagca taacgcttgt agttgatatt tgagatcccc
     2101 tatcattgca gggtgacagc ggagcggctt cgcagagctg cattaaccag ggcttcgggc
     2161 aggccaaaaa ctacggcacg ctccggccac ccagtccgcc ggaggactcc ggttcaggga
     2221 gcggccaact agccgagaac ctcacctatg cctggcacaa tatggacatc tttggggcgg
     2281 tcaatcagcc gggctccgga tggcggcagc tggtcaaccg gacacgcgga ctattctgca
     2341 acgagcgaca cataccggcg cccaggaaac atttgctcaa gaacggtgag tttctattcg
     2401 cagtcggctg atctgtgtga aatcttaata aagggtccaa ttaccaattt gaaactcagt
     2461 ttgcggcgtg gcctatccgg gcgaactttt ggccgtgatg ggcagttccg gtgccggaaa
     2521 gacgaccctg ctgaatgccc ttgcctttcg atcgccgcag ggcatccaag tatcgccatc
     2581 cgggatgcga ctgctcaatg gccaacctgt ggacgccaag gagatgcagg ccaggtgcgc
     2641 ctatgtccag caggatgacc tctttatcgg ctccctaacg gccagggaac acctgatttt
     2701 ccaagccatg gtgcggatgc cacgacatct gacctatcgg cagcgagtgg cccgcgtgga
     2761 tcaggtgatc caggagcttt cgctcagcaa atgtcagcac acgatcatcg gtgtgcccgg
     2821 cagggtgaaa ggtctgtccg gcggagaaag gaagcgtctg gcattcgcct ccgaggctct
     2881 aaccgatccg ccgcttctga tctgcgatga gcccacctcc ggactggact cctttaccgc
     2941 ccacagcgtc gtccaggtgc tgaagaagct gtcgcagaag ggcaagaccg tcatcctgac
     3001 cattcatcag ccgtcttccg agctgtttga gctctttgac aagatccttc tgatggccga
     3061 gggcagggta gctttcttgg gcactcccag cgaagccgtc gacttctttt cctagtgagt
     3121 tcgatgtgtt tattaagggt atctagtatt acataacatc tcaactccta tccagcgtgg
     3181 gtgcccagtg tcctaccaac tacaatccgg cggactttta cgtacaggtg ttggccgttg
     3241 tgcccggacg ggagatcgag tcccgtgatc ggatcgccaa gatatgcgac aattttgcca
     3301 ttagcaaagt agcccgggat atggagcagt tgttggccac caaaaatctg gagaagccac
     3361 tggagcagcc ggagaatggg tacacctaca aggccacctg gttcatgcag ttccgggcgg
     3421 tcctgtggcg atcctggctg tcggtgctca aggaaccact cctcgtaaaa gtgcgactta
     3481 ttcagacaac ggtgagtggt tccagtggaa acaaatgata taacgcttac aattcttgga
     3541 aacaaattcg ctagatttta gatagaattg cctgattcca cacccttctt agtttttttc
     3601 aatgagatgt atagtttata gttttgcaga agataaataa atttcattta actcgcgaat
     3661 attaatgaga tgcgagtaac attttaattt gcagatggtt gccatcttga ttggcctcat
     3721 ctttttgggc caacaactca cgcaagtggg tgtgatgaat atcaacggag ccatcttcct
     3781 cttcctgacc aacatgacct ttcaaaacgt ctttgccacg ataaatgtaa gtcatgttta
     3841 gaatacattt gcatttcaat aatttactaa ctttctaatg aatcgattcg atttaggtgt
     3901 tcacctcaga gctgccagtt tttatgaggg aggcccgaag tcgactttat cgctgtgaca
     3961 catactttct gggcaaaacg attgccgaat tgccgctttt tctcacagtg ccactggtct
     4021 tcacggcgat tgcctatccg atgatcggac tgcgggccgg agtgctgcac ttcttcaact
     4081 gcctggcgct ggtcactctg gtggccaatg tgtcaacgtc cttcggatat ctaatatcct
     4141 gcgccagctc ctcgacctcg atggcgctgt ctgtgggtcc gccggttatc ataccattcc
     4201 tgctctttgg cggcttcttc ttgaactcgg gctcggtgcc agtatacctc aaatggttgt
     4261 cgtacctctc atggttccgt tacgccaacg agggtctgct gattaaccaa tgggcggacg
     4321 tggagccggg cgaaattagc tgcacatcgt cgaacaccac gtgccccagt tcgggcaagg
     4381 tcatcctgga gacgcttaac ttctccgccg ccgatctgcc gctggactac gtgggtctgg
     4441 ccattctcat cgtgagcttc cgggtgctcg catatctggc tctaagactt cgggcccgac
     4501 gcaaggagta gccgacatat atccgaaata actgcttgtt tttttttttt accattatta
     4561 ccatcgtgtt tactgtttat tgccccctca aaaagctaat gtaattatat ttgtgccaat
     4621 aaaaacaaga tatgacctat agaatacaag tatttcccct tcgaacatcc ccacaagtag
     4681 actttggatt tgtcttctaa ccaaaagact tacacacctg cataccttac atcaaaaact
     4741 cgtttatcgc tacataaaac accgggatat attttttata tacatacttt tcaaatcgcg
     4801 cgccctcttc ataattcacc tccaccacac cacgtttcgt agttgctctt tcgctgtctc
     4861 ccacccgctc tccgcaacac attcaccttt tgttcgacga ccttggagcg actgtcgtta
     4921 gttccgcgcg attcggttcg ctcaaatggt tccgagtggt tcatttcgtc tcaatagaaa
     4981 ttagtaataa atatttgtat gtacaattta tttgctccaa tatatttgta tatatttccc
     5041 tcacagctat atttattcta atttaatatt atgacttttt aaggtaattt tttgtgacct
     5101 gttcggagtg attagcgtta caatttgaac tgaaagtgac atccagtgtt tgttccttgt
     5161 gtagatgcat ctcaaaaaaa tggtgggcat aatagtgttg tttatatata tcaaaaataa
     5221 caactataat aataagaata catttaattt agaaaatgct tggatttcac tggaactagg
     5281 gcgcgcctag gtcgtgaagc ttgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     5341 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     5401 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     5461 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg aaattgtaag cgttaatatt
     5521 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat ttttttaacc aataggccga
     5581 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     5641 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     5701 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     5761 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     5821 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     5881 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     5941 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     6001 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     6061 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     6121 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     6181 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     6241 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     6301 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     6361 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     6421 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     6481 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     6541 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     6601 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     6661 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     6721 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     6781 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     6841 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     6901 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     6961 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     7021 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     7081 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     7141 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     7201 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     7261 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     7321 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     7381 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     7441 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     7501 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     7561 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     7621 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     7681 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     7741 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     7801 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     7861 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     7921 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     7981 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     8041 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     8101 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
     8161 ggagctcgtt gttggttggc acaccacaaa tatactgttg ccgagcacaa ttgatcggct
     8221 aaatggtatg gcaagaaaag gtatgcaata taataatctt ttattgggta tgcaacgaaa
     8281 atttgtttcg tcaacgtatg caatattctt tattaaaaga gggtatgcaa tgtattttat
     8341 taaaaacggg tatgcaatat aataatcttt tattgggtat gcaacgaaaa tttgtttcgt
     8401 caaagtatgc aatatttttt attaaaagag ggtatgcaat gtattttatt aaaaacgggt
     8461 atgcaataaa aaattatttg gtttctctaa aaagtatgca gcacttattt tttgataagg
     8521 tatgcaacaa aattttactt tgccgaaaat atgcaatgtt tttgcgaata aattcaacgc
     8581 acacttatta cgtggccaga gctctggaac cagacatgat aagatacatt gatgagtttg
     8641 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     8701 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattccattc
     8761 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
     8821 acaaatgtgg tatggctgat tatgatctct agtcaaggca ctatacatca aatatttttc
     8881 cataattttc ttgtatagca gtgcagcttt ttcctttgtg gtgtaaatag caaagcaagc
     8941 aagagttcta ttactaaaca cagcatgact caaaaaactt agcaattctg aaggaaagtc
     9001 cttggggtct tctacctttc tcttcttttt tggaggagta gaatgttgag agtcagcagt
     9061 agcctcatca tcactagatg gcatttcttc tgagcaaaac aggttttcct cattaaaggc
     9121 attccaccac tgctcccatt catcagttcc ataggttgga atctaaaata cacaaacaat
     9181 tagaatcagt agtttaacac attatacact taaaaatttt atatttacct tagagcttta
     9241 aatctctgta ggtagtttgt ccaattatgt cacaccacag aagtaaggtt ccttcacaaa
     9301 gatcccgcgg ctctagttct ttgcaatctg taagcataag caaagaaaaa atgggttagc
     9361 taatgagttt tataactgcg tattatttga aaatatcaaa aatgtataca aaaacatatt
     9421 ttcaatattt tcaaagtgta tttttagggg ttgttaatcg tgtgtgatgc ctacctgatg
     9481 ccaacaattg tctagcagat cagaactagt ttgctctaga ggtaccctcg aatcaccact
     9541 ttgtacaaga aagctgggtc ggcgcgccca cccttttaga ctttcctctt cttcttgggc
     9601 tcgaattcgc tgccgtcggc ggttcttttt gagccgccag agggtgatga attttctatg
     9661 aggagggtag aggtgtctgg agtctctgtg atggctgcct ttcgggccgc ttggtcagcc
     9721 atccggttgc ctctagcctc ggcgctgtgt cccttttgat gtcctggaca atggattatg
     9781 ctaagtcttt tgggcagaaa gagggctttt agtagggcca agatctcgtc tttatttttg
     9841 atctctttgc cttctgatgt gagccaccca cgccttctgt atatttctcc atggatatgg
     9901 gcagtagcaa aagcataacg gctatcagta taaacattta gcttcttacc ttctgccatc
     9961 tttagggcct gggtgagtgc tatcagttca gcccgctgag cggatgtccc ggctggcagg
    10021 gctttagccc agattacctc ggtctcggtg gtcaccgcag ctcccgcctt acgctgtccc
    10081 tcttgtaaga gactgcttcc atccgtgtac caggtgtggt cggcgtctgg gagcggctgg
    10141 tccgttaggt cgggtcgggt tccgtgggct tcggccagga tatcaaggca gttgtgttgc
    10201 agcccttcct caggcagtgg gagcagcgta gccgggttca gggctaccac cggtccgaac
    10261 tggacccggt ccgtgtccaa aagcaaggcc tgatagtgag tcatccgggc gttggaaagc
    10321 cagcggtcgg ggggttgttt gactagtgcc tctactgcat ggggggccag aatgactagt
    10381 ggctgtccca tggttagctt gcctgcatcc tttgtcagta cggcaatggc tgctaccatc
    10441 cgtaggcaag ggggccaccc agctgctact gggtctagct ttttggacag gtaggccacc
    10501 ggccgacgcc aaggtcccag tttttgcgtt aggacacctt tggcgtagcc ctgcttctcg
    10561 tcgacaaaga gttcaaaggg cttagtcaaa tctggcaacc ccagggctgg ggcagttaga
    10621 agagcttgct tgatttcttg ataggccttt tgttggtctg ggccccaatt aaacagagtc
    10681 cccggtttgg tgagagggta caggggggct gccatttctg caaacccagg gatgaagagg
    10741 cgacagaagc ctgccttccc taggaactcc cttagttgtc gaggggtctt cggagtaggc
    10801 tgccccatca cagtctcttt tctggcctca gtcagccatc tctgaccctc ttttagaaga
    10861 taccccagat acttgacctg tttctggcaa atttgggctt tcttggccga ggcccgatac
    10921 ccgaggttcc ctagggtttg taacagggcc cgagtacctt gttggcagtc tagctcagaa
    10981 gtggcggcca gcagtaagtc atccacgtac tgtagcagga tcaagtctgg gtgctggatc
    11041 cggaagtctg ctaggtctct gtgcagtgcc tcattaaaca gggtgggact gtttttgaaa
    11101 ccctgtggga gtctggtcca ggtcaattgt cctgagattc ccatctctgg atctctccac
    11161 tcaaaggcga agagaggctg actggtgggg tggagtctca ggcagaaaaa ggcatccttt
    11221 aaatcaagca cagtgtacca ctggtgggac ggtgggagcc cgctcaagag gttgtaaggg
    11281 ttgggcacgg tggggtggat gtcttccacc cgcttgttga cttctctcag atcctggaca
    11341 ggcctataat cattagtccc tggtttctta acgggtagca ggggcgtgtt ccagggggac
    11401 tggcagggta ccagtattcc ctggtccaac agtctctgta tgtggggctt gatccccagt
    11461 ctggcttctt gtgacatggg gtattgtttt atggacacgg gggtagaggt tgctttcaga
    11521 ggtatgatca gaggagcttg gcgaactgcc agtcccatgc ccccggtttc cgcccaggcc
    11581 tgaggaaaat cagacagcca tgtggaccct agagaaacat ctggctcttt tgaggtctca
    11641 tgtagccgat actcatcttc tatatttagg gtgctgctgc cgccgctgct gccgccactg
    11701 ctctctggtg ttgctgactc gcttgttcct ggtgtctcgc tgccagagga tcctccgcta
    11761 gatcctccag agtcacctcc cagctgagac aggtcgatcc gtgtctcgta caggccggtg
    11821 atgctctggt ggatcagggt ggcgtccagc acctctttgg tgctggtgta cctcttccgg
    11881 tcgatggtgg tgtcaaagta cttgaaggcg gcaggggctc ccagattggt cagggtaaac
    11941 aggtggatga tattctcggc ctgctctctg atgggcttat cccggtgctt gttgtaggcg
    12001 gacagcactt tgtccagatt agcgtcggcc aggatcactc tcttggagaa ctcgctgatc
    12061 tgctcgatga tctcgtccag gtagtgcttg tgctgttcca caaacagctg tttctgctca
    12121 ttatcctcgg gggagccctt cagcttctca tagtggctgg ccaggtacag gaagttcaca
    12181 tatttggagg gcagggccag ttcgtttccc ttctgcagtt cgccggcaga ggccagcatt
    12241 ctcttccggc cgttttccag ctcgaacagg gagtacttag gcagcttgat gatcaggtcc
    12301 tttttcactt ctttgtagcc cttggcttcc agaaagtcga tgggattctt ctcgaagctg
    12361 cttctttcca tgatggtgat ccccagcagc tctttcacac tcttcagttt cttggacttg
    12421 cccttttcca ctttggccac caccagcaca gaataggcca cggtggggct gtcgaagccg
    12481 ccgtacttct tagggtccca gtccttcttt ctggcgatca gcttatcgct gttcctcttg
    12541 ggcaggatag actctttgct gaagccgcct gtctgcacct cggtcttttt cacgatattc
    12601 acttggggca tgctcagcac tttccgcacg gtggcaaaat cccggccctt atcccacacg
    12661 atctccccgg tttcgccgtt tgtctcgatc agaggccgct tccggatctc gccgttggcc
    12721 agggtaatct cggtcttgaa aaagttcatg atgttgctgt agaagaagta cttggcggta
    12781 gccttgccga tttcctgctc gctcttggcg atcatcttcc gcacgtcgta caccttgtag
    12841 tcgccgtaca cgaactcgct ttccagctta gggtactttt tgatcagggc ggttcccacg
    12901 acggcgttca ggtaggcgtc gtgggcgtgg tggtagttgt tgatctcgcg cactttgtaa
    12961 aactggaaat ccttccggaa atcggacacc agcttggact tcagggtgat cactttcact
    13021 tcccggatca gcttgtcatt ctcgtcgtac ttagtgttca tccgggagtc caggatctgt
    13081 gccacgtgct ttgtgatctg ccgggtttcc accagctgtc tcttgatgaa gccggcctta
    13141 tccagttcgc tcaggccgcc tctctcggcc ttggtcagat tgtcgaactt tctctgggta
    13201 atcagcttgg cgttcagcag ctgccgccag tagttcttca tcttcttcac gacctcttcg
    13261 gagggcacgt tgtcgctctt gccccggttc ttgtcgcttc tggtcagcac cttgttgtcg
    13321 atggagtcgt ccttcagaaa gctctgaggc acgatagcgt ccacatcgta gtcggacagc
    13381 cggttgatgt ccagttcctg gtccacgtac atatcccgcc cattctgcag gtagtacagg
    13441 tacagcttct cgttctgcag ctgggtgttt tccacggggt gttctttcag gatctggctg
    13501 cccagctctt tgatgccctc ttcgatccgc ttcattctct cgcggctgtt cttctgtccc
    13561 ttctgggtgg tctggttctc tctggccatt tcgatcacga tgttctcggg cttgtgccgg
    13621 cccatcactt tcacgagctc gtccaccacc ttcactgtct gcaggatgcc cttcttaatg
    13681 gcggggctgc cggccagatt ggcaatgtgc tcgtgcaggc tatcgccctg gccggacacc
    13741 tgggctttct ggatgtcctc tttaaaggtc aggctgtcgt cgtggatcag ctgcatgaag
    13801 tttctgttgg cgaagccgtc ggacttcagg aaatccagga ttgtcttgcc ggactgcttg
    13861 tcccggatgc cgttgatcag cttccggctc agcctgcccc agccggtgta tctccgccgc
    13921 ttcagctgct tcatcacttt gtcgtcgaac aggtgggcat aggttttcag ccgttcctcg
    13981 atcatctctc tgtcctcaaa cagtgtcagg gtcagcacga tatcttccag aatgtcctcg
    14041 ttttcctcat tgtccaggaa gtccttgtcc ttgataattt tcagcagatc gtggtatgtg
    14101 cccagggagg cgttgaaccg atcttccacg ccggagattt ccacggagtc gaagcactcg
    14161 attttcttga agtagtcctc tttcagctgc ttcacggtca ctttccggtt ggtcttgaac
    14221 agcaggtcca cgatggcctt tttctgctcg ccgctcagga aggcgggctt tctcattccc
    14281 tcggtcacgt atttcacttt ggtcagctcg ttatacacgg tgaagtactc gtacagcagg
    14341 ctgtgcttgg gcagcacctt ctcgttgggc aggttcttat cgaagttggt catccgctcg
    14401 atgaagctct gggcggaagc gcccttgtcc accacttcct cgaagttcca gggggtgatg
    14461 gtttcctcgc tctttctggt catccaggcg aatctgctgt ttcccctggc cagagggccc
    14521 acgtagtagg ggatgcggaa ggtcaggatc ttctcgatct tttcccggtt gtccttcagg
    14581 aatgggtaaa aatcttcctg ccgccgcaga atggcgtgca gctctcccag gtggatctgg
    14641 tgggggatgc tgccgttgtc gaaggtccgc tgcttccgca gcaggtcctc tctgttcagc
    14701 ttcacgagca gttcctcggt gccgtccatc ttttccagga tgggcttgat gaacttgtag
    14761 aactcttcct ggctggctcc gccgtcaatg tagccggcgt agccgttctt gctctggtcg
    14821 aagaaaatct ctttgtactt ctcaggcagc tgctgccgca cgagagcttt cagcagggtc
    14881 aggtcctggt ggtgctcgtc gtatctcttg atcatagagg cgctcagggg ggccttggtg
    14941 atctcggtgt tcactctcag gatgtcgctc agcaggatgg cgtcggacag gttcttggcg
    15001 gccagaaaca ggtcggcgta ctggtcgccg atctgggcca gcaggttgtc caggtcgtcg
    15061 tcgtaggtgt ccttgctcag ctgcagtttg gcatcctcgg ccaggtcgaa gttgctcttg
    15121 aagttggggg tcaggcccag gctcagggca atcaggtttc cgaacaggcc attcttcttc
    15181 tcgccgggca gctgggcgat cagattttcc agccgtctgc tcttgctcag tctggcagac
    15241 aggatggcct tggcgtccac gccgctggcg ttgatggggt tttcctcgaa cagctggttg
    15301 taggtctgca ccagctggat gaacagcttg tccacgtcgc tgttgtcggg gttcaggtcg
    15361 ccctcgatca ggaagtggcc ccggaacttg atcatgtggg ccagggccag atagatcagc
    15421 cgcaggtcgg ccttgtcggt gctgtccacc agtttctttc tcaggtggta gatggtgggg
    15481 tacttctcgt ggtaggccac ctcgtccacg atgttgccga agatggggtg ccgctcgtgc
    15541 ttcttatcct cttccaccag gaaggactct tccagtctgt ggaagaagct gtcgtccacc
    15601 ttggccatct cgttgctgaa gatctcttgc agatagcaga tccggttctt ccgtctggtg
    15661 tatcttcttc tggcggttct cttcagccgg gtggcctcgg ctgtttcgcc gctgtcgaac
    15721 agcagggctc cgatcaggtt cttcttgatg ctgtgccggt cggtgttgcc cagcaccttg
    15781 aatttcttgc tgggcacctt gtactcgtcg gtgatcacgg cccagcccac agagttggtg
    15841 ccgatgtcca ggccgatgct gtacttcttg tcgactttcc gcttcttctt tggtgactcg
    15901 aactcgcttc cgtcggctgt ccgtttcatg gtgaaggggg cggccgcgga gcctgctttt
    15961 ttgtacaaac ttgtgattcg agccgcggcc gcagatctgc gaattcccaa ttccctattc
    16021 agagttctct tcttgtattc aataattact tcttggcaga tttcagtagt tgcagttgat
    16081 ttacttggtt gctggttact tttaattgat tcactttaac ttgcacttta ctgcagattg
    16141 tttagcttgt tcagctgcgc ttgtttattt gcttagcttt cgcttagcga cgtgttcact
    16201 ttgcttgttt gaattgaatt gtcgctccgt agacgaagcg cctctattta tactccggcg
    16261 ctcgctgtcg acgagtctcc gctcgga
//
