LOCUS       sequence-30643-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Mak AB, Ni Z, Hewel JA, Chen GI, Zhong G, Karamboulas K, Blakely K, 
            Smiley S, Marcon E, Roudeva D, Li J, Olsen JB, Punna T, Isserlin R, 
            Chetyrkin S, Gingras AC, Emili A, Greenblatt J, Moffat J
  TITLE     A lentiviral-based functional proteomics approach identifies 
            chromatin remodelling complexes important for the induction of 
            pluripotency.
  JOURNAL   Mol Cell Proteomics. 2010 May;9(5):811-23.
  PUBMED    20305087
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2010-05"; volume: "9(5)"; pages: "811-23"
COMMENT     Sequence Label: pLD-puro-CnVA
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             64..129
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             139..159
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             172..192
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             205..222
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             235..258
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             295..318
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    400..524
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(549..554,555..572,573..579)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(699..718)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gagctcgttt agtgaaccgt cangatcgcc tggagacgcc atccacgctg tgctagcacc
       61 atggactaca aagaccatga cggtgattat aaagatcatg acatcgatta caaggatgac
      121 gatgacaagc ctgcaggaga aaatctgtac ttccagggga gaggaggcag cgagaacctg
      181 tactttcagg ggcctgcagg aagacatcac catcaccatc acagcggcgg aagatggagc
      241 caccctcagt tcgagaaggg aggaggaagc ggcggaggca gcggaggagg aagctggagc
      301 cacccgcagt tcgagaaagc gatcgccaga gagctgttca acctgctggg cgagaaccag
      361 ccccccgtgg tgatcaaggc gatcgccacc ggtaagctta caagtttgta caaaaaagct
      421 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
      481 cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca
      541 ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc
      601 cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca
      661 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc
      721 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga
      781 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg at
//
