LOCUS       sequence-306432-       11320 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac transposon vector with CAG promoter expressing SMARCA4 
            (T910M mutant)-Venus fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11320)
  AUTHORS   Ballabio C, Anderle M, Gianesello M, Lago C, Miele E, Cardano M, 
            Aiello G, Piazza S, Caron D, Gianno F, Ciolfi A, Pedace L, 
            Mastronuzzi A, Tartaglia M, Locatelli F, Ferretti E, Giangaspero F, 
            Tiberi L
  TITLE     Modeling medulloblastoma in vivo and with human cerebellar 
            organoids.
  JOURNAL   Nat Commun. 2020 Jan 29;11(1):583. doi: 10.1038/s41467-019-13989-3.
  PUBMED    31996670
REFERENCE   2  (bases 1 to 11320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-01-29"; volume: "11(1):583. doi"; pages: " 
            10.1038/s41467-019-13989-3"
COMMENT     Sequence Label: pPB CAG SMARCA4 T910M-Venus
FEATURES             Location/Qualifiers
     source          1..11320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(96..887,888..956))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     719..738
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(957..1061)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1087..1542)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1224..1245)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1436..1455
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1669..1691
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1683..1700
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1684..1700
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1707..1726
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1707..1725
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   1744..1778
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        2061..2440
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2442..2719
                     /label=chicken beta-actin promoter
     intron          2720..3736
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3660..3682
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3744..3763
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(8673..8675,8676..9389)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8715..8736)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8976..8995)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9323..9344
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(9483..9502)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9548..9603
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9602..9621)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     repeat_region   10131..10193
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(10217..10235)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10256..10272)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10256..10272)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10269..10291)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10280..10296
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10304..10310,10311..10328,10329..10334))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10349..10370
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10487..10504)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10658..11245)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10737..10756)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
       61 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
      121 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
      181 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
      241 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
      301 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
      361 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
      421 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
      481 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
      541 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
      601 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
      661 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
      721 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
      781 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
      841 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
      901 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
      961 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     1021 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     1081 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     1141 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     1201 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     1261 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     1321 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     1381 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     1441 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     1501 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
     1561 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     1621 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     1681 gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa ttgggtacct
     1741 taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat attgctctct
     1801 ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg ccgcttggag
     1861 ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact ataacgaccg
     1921 cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta actcatacga
     1981 taattatatt gttatttcat gttctactta cgtgataact tattatatat atattttctt
     2041 gttatagata tcgtcgggtc gacattgatt attgactagt tattaatagt aatcaattac
     2101 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     2161 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     2221 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     2281 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     2341 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     2401 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
     2461 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
     2521 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc caggcggggc
     2581 ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag
     2641 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa
     2701 gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc
     2761 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg
     2821 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc
     2881 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag
     2941 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct
     3001 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg
     3061 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa
     3121 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg
     3181 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc
     3241 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt
     3301 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg
     3361 cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg
     3421 gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc
     3481 tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag
     3541 gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct
     3601 ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg
     3661 gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt
     3721 cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg
     3781 caaagaattg catgtccact ccagacccac ccctgggcgg aactcctcgg ccaggtcctt
     3841 ccccgggccc tggcccttcc cctggagcca tgctgggccc tagcccgggt ccctcgccgg
     3901 gctccgccca cagcatgatg gggcccagcc cagggccgcc ctcagcagga caccccatcc
     3961 ccacccaggg gcctggaggg taccctcagg acaacatgca ccagatgcac aagcccatgg
     4021 agtccatgca tgagaagggc atgtcggacg acccgcgcta caaccagatg aaaggaatgg
     4081 ggatgcggtc agggggccat gctgggatgg ggcccccgcc cagccccatg gaccagcact
     4141 cccaaggtta cccctcgccc ctgggtggct ctgagcatgc ctctagtcca gttccagcca
     4201 gtggcccgtc ttcggggccc cagatgtctt ccgggccagg aggtgccccg ctggatggtg
     4261 ctgaccccca ggccttgggg cagcagaacc ggggcccaac cccatttaac cagaaccagc
     4321 tgcaccagct cagagctcag atcatggcct acaagatgct ggccaggggg cagcccctcc
     4381 ccgaccacct gcagatggcg gtgcagggca agcggccgat gcccgggatg cagcagcaga
     4441 tgccaacgct acctccaccc tcggtgtccg caacaggacc cggccctggc cctggccctg
     4501 gccccggccc gggtcccggc ccggcacctc caaattacag caggcctcat ggtatgggag
     4561 ggcccaacat gcctccccca ggaccctcgg gcgtgccccc cgggatgcca ggccagcctc
     4621 ctggagggcc tcccaagccc tggcctgaag gacccatggc gaatgctgct gcccccacga
     4681 gcacccctca gaagctgatt cccccgcagc caacgggccg cccttccccc gcgccccctg
     4741 ccgtcccacc cgccgcctcg cccgtgatgc caccgcagac ccagtccccc gggcagccgg
     4801 cccagcccgc gcccatggtg ccactgcacc agaagcagag ccgcatcacc cccatccaga
     4861 agccgcgggg cctcgaccct gtggagatcc tgcaggagcg cgagtacagg ctgcaggctc
     4921 gcatcgcaca ccgaattcag gaacttgaaa accttcccgg gtccctggcc ggggatttgc
     4981 gaaccaaagc gaccattgag ctcaaggccc tcaggctgct gaacttccag aggcagctgc
     5041 gccaggaggt ggtggtgtgc atgcggaggg acacagcgct ggagacagcc ctcaatgcta
     5101 aggcctacaa gcgcagcaag cgccagtccc tgcgcgaggc ccgcatcact gagaagctgg
     5161 agaagcagca gaagatcgag caggagcgca agcgccggca gaagcaccag gaatacctca
     5221 atagcattct ccagcatgcc aaggatttca aggaatatca cagatccgtc acaggcaaaa
     5281 tccagaagct gaccaaggca gtggccacgt accatgccaa cacggagcgg gagcagaaga
     5341 aagagaacga gcggatcgag aaggagcgca tgcggaggct catggctgaa gatgaggagg
     5401 ggtaccgcaa gctcatcgac cagaagaagg acaagcgcct ggcctacctc ttgcagcaga
     5461 cagacgagta cgtggctaac ctcacggagc tggtgcggca gcacaaggct gcccaggtcg
     5521 ccaaggagaa aaagaagaaa aagaaaaaga agaaggcaga aaatgcagaa ggacagacgc
     5581 ctgccattgg gccggatggc gagcctctgg acgagaccag ccagatgagc gacctcccgg
     5641 tgaaggtgat ccacgtggag agtgggaaga tcctcacagg cacagatgcc cccaaagccg
     5701 ggcagctgga ggcctggctc gagatgaacc cggggtatga agtagctccg aggtctgata
     5761 gtgaagaaag tggctcagaa gaagaggaag aggaggagga ggaagagcag ccgcaggcag
     5821 cacagcctcc caccctgccc gtggaggaga agaagaagat tccagatcca gacagcgatg
     5881 acgtctctga ggtggacgcg cggcacatca ttgagaatgc caagcaagat gtcgatgatg
     5941 aatatggcgt gtcccaggcc cttgcacgtg gcctgcagtc ctactatgcc gtggcccatg
     6001 ctgtcactga gagagtggac aagcagtcag cgcttatggt caatggtgtc ctcaaacagt
     6061 accagatcaa aggtttggag tggctggtgt ccctgtacaa caacaacctg aacggcatcc
     6121 tggccgacga gatgggcctg gggaagacca tccagaccat cgcgctcatc acgtacctca
     6181 tggagcacaa acgcatcaat gggcccttcc tcatcatcgt gcctctctca acgctgtcca
     6241 actgggcgta cgagtttgac aagtgggccc cctccgtggt gaaggtgtct tacaagggat
     6301 ccccagcagc aagacgggcc tttgtccccc agctccggag tgggaagttc aacgtcttgc
     6361 tgacgacgta cgagtacatc atcaaagaca agcacatcct cgccaagatc cgttggaagt
     6421 acatgattgt ggacgaaggt caccgcatga agaaccacca ctgcaagctg acgcaggtgc
     6481 tcaacacgca ctatgtggca ccccgccgcc tgctgctgat gggcacaccg ctgcagaaca
     6541 agcttcccga gctctgggcg ctgctcaact tcctgctgcc caccatcttc aagagctgca
     6601 gcaccttcga gcagtggttt aacgcaccct ttgccatgac cggggaaaag gtggacctga
     6661 atgaggagga aaccattctc atcatccggc gtctccacaa agtgctgcgg cccttcttgc
     6721 tccgacgact caagaaggaa gtcgaggccc agttgcccga aaaggtggag tacgtcatca
     6781 agtgcgacat gtctgcgctg cagcgagtgc tctaccgcca catgcaggcc aagggcgtgc
     6841 tgctgactga tggctccgag aaggacaaga agggcaaagg cggcaccaag accctgatga
     6901 acaccatcat gcagctgcgg aagatctgca accaccccta catgttccag cacatcgagg
     6961 agtccttttc cgagcacttg gggttcactg gcggcattgt ccaagggctg gacctgtacc
     7021 gagcctcggg taaatttgag cttcttgata gaattcttcc caaactccga gcaaccaacc
     7081 acaaagtgct gctgttctgc caaatgacct ccctcatgac catcatggaa gattactttg
     7141 cgtatcgcgg ctttaaatac ctcaggcttg atggaaccac gaaggcggag gaccggggca
     7201 tgctgctgaa aaccttcaac gagcccggct ctgagtactt catcttcctg ctcagcaccc
     7261 gggctggggg gctcggcctg aacctccagt cggcagacac tgtgatcatt tttgacagcg
     7321 actggaatcc tcaccaggac ctgcaagcgc aggaccgagc ccaccgcatc gggcagcaga
     7381 acgaggtgcg tgtgctccgc ctctgcaccg tcaacagcgt ggaggagaag atcctagctg
     7441 cagccaagta caagctcaac gtggaccaga aggtgatcca ggccggcatg ttcgaccaga
     7501 agtcctcaag ccatgagcgg cgcgccttcc tgcaggccat cctggagcac gaggagcagg
     7561 atgaggagga agacaaggtg cccgacgacg agaccgtcaa ccagatgatc gcccggcacg
     7621 aggaggagtt tgatctgttc atgcgcatgg acctggaccg caggcgcgag gaggcccgca
     7681 accccaagcg gaagccgcgc ctcatggagg aggacgagct cccctcgtgg atcatcaagg
     7741 acgacgcgga ggtggagcgg ctgacctgtg aggaggagga ggagaagatg ttcggccgtg
     7801 gctcccgcca ccgcaaggag gtggactaca gcgactcact gacggagaag cagtggctca
     7861 aggccatcga ggagggcacg ctggaggaga tcgaagagga ggtccggcag aagaaatcat
     7921 cacggaagcg caagcgagac agcgacgccg gctcctccac cccgaccacc agcacccgca
     7981 gccgcgacaa ggacgacgag agcaagaagc agaagaagcg cgggcggccg cctgccgaga
     8041 aactctcccc taacccaccc aacctcacca agaagatgaa gaagattgtg gatgccgtga
     8101 tcaagtacaa ggacagcagc agtggacgtc agctcagcga ggtcttcatc cagctgccct
     8161 cgcgaaagga gctgcccgag tactacgagc tcatccgcaa gcccgtggac ttcaagaaga
     8221 taaaggagcg cattcgcaac cacaagtacc gcagcctcaa cgacctagag aaggacgtca
     8281 tgctcctgtg ccagaacgca cagaccttca acctggaggg ctccctgatc tatgaagact
     8341 ccatcgtctt gcagtcggtc ttcaccagcg tgcggcagaa aatcgagaag gaggatgaca
     8401 gtgaaggcga ggagagtgag gaggaggaag agggcgagga ggaaggctcc gaatccgaat
     8461 ctcggtccgt caaagtgaag atcaagcttg gccggaagga gaaggcacag gaccagctga
     8521 agggcggccg gcggcggccg agccgagggt cccgagccaa gccggtcgtg agtgacgatg
     8581 acagtgagga ggaacaagag gaggaccgct caggaagtgg cagcgaagaa gacaccggtg
     8641 gttctggtgg tggttctggt ggtggttctg gtgtgagcaa gggcgaggag ctgttcaccg
     8701 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     8761 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagctg atctgcacca
     8821 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgggctac ggcctgcagt
     8881 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     8941 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     9001 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     9061 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     9121 tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca
     9181 acatcgagga cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     9241 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag
     9301 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     9361 ctctcggcat ggacgagctg tacaagtgat gagcggccgc aattcactcc tcaggtgcag
     9421 gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga
     9481 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact
     9541 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
     9601 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
     9661 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga
     9721 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg
     9781 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta
     9841 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc
     9901 atagctgtcc ctcttctctt atggagatcc ctcgacctgc accgtctatg gagatcggat
     9961 cctttgttac tttatagaag aaattttgag tttttgtttt tttttaataa ataaataaac
    10021 ataaataaat tgtttgttga atttattatt agtatgtaag tgtaaatata ataaaactta
    10081 atatctattc aaattaataa ataaacctcg atatacagac cgataaaaca catgcgtcaa
    10141 ttttacgcat gattatcttt aacgtacgtc acaatatgat tatctttcta gggttaaaga
    10201 gctccagctt ttgttccctt tagtgagggt taatttcgag cttggcgtaa tcatggtcat
    10261 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
    10321 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
    10381 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
    10441 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
    10501 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
    10561 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
    10621 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    10681 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aacccgacag gactataaag
    10741 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    10801 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    10861 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    10921 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    10981 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    11041 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    11101 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    11161 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    11221 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    11281 tcagtggaac gaaaactcac gttaagggat tttggtcatg
//
