LOCUS       sequence-306458-       11333 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  parental, all-in-one CRISPRi vector for B. burgdorferi, parental for
            gRNA cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11333)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 11333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9K_arr2
FEATURES             Location/Qualifiers
     source          1..11333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(3218..3232,3233..3238,3239..3255,3256..3262)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    3238..3254
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    3270..3286
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3305..3316
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(3323..3349,3350..3352,3353..5839,5840..5842,
                     5843..7429)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7599..8408
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MGHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7666..7685)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      8531..9119
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9020..9039
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 attttgatta tattatcttt atcaatattt aaattgaata aaattgtaag aaaaattgaa
       61 agaatttttt tacataaaaa gttacattta ttgaagtaat ttcttacttg atacttttct
      121 atttcattaa tctttctttc ttcttttata ttatttttat tatttaaaca ctcctttaaa
      181 tctacactcc catttttagt aaatctatct ttaaggcccc ttgaaactct attttgaaat
      241 cttaaatctt ttttttcttg aaagtattta tagatttttt ggtaacattc tttttttgaa
      301 aaattaagtt gatagtatat ttcggtcccc aaatttaccc ctaagtgttt gtgataatta
      361 gttgtgactt taaattcttt ttctaatttg taaaggtaat tttgcagtgt ttttagttta
      421 acgggtgttt gactatttct tttaaggttt tcattaaagt aatagagtat gtttttttgt
      481 gtgtatttct tatatgtttt atttatgtac gctagtgttg agataaggac tactaattta
      541 tactgtaatt tattgtagca agttgtggtt ttactggttt tgtttatatt agataagttt
      601 tccatttata gctccttgaa taccggattt tttatctata aagtatatat atcacaattt
      661 tgatttaaaa gtaaattctt tttttaaaaa tacaaaaatt ttttataaat tatatagacc
      721 atgatattgt attaatacta attgatttta atttaaggtt gaactaaaat aaacatagtt
      781 ttgtagaaaa taatttttca ttattttgct acttgatatt ggatcgtaaa aatattaggg
      841 ctttacaaag ttcttttaaa agagaattta gtaaagccct aagtctttta ataaaaaatt
      901 ttttgtaaaa aagttggcaa aaatagtttt tgctatatat ttatatataa gaaaaaattc
      961 aatactcaag gagataaaaa atggaaaact taaacaataa tccacaaaaa attaatcaaa
     1021 taaataagca aactaatttt attggttttg aaaattttat agaacttgat aatcaagtta
     1081 atcaaactaa agaaaaaagt aaattaagta agataggtaa gaaattaccc ggaataagca
     1141 gtcaagtcta gtgcggccgc atggcttgtt atgactgttt ttttggggta cagtctatgc
     1201 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     1261 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgaggaggt
     1321 aacgtcatgg taaaagattg gattcctatt tctcatgata attataaaca agtacaagga
     1381 ccattttatc atggaactaa agctaattta gcaattggtg atttactaac tacaggattt
     1441 atttctcatt ttgaagatgg tcgaattctt aaacatattt atttttcagc tttaatggaa
     1501 ccagcagttt ggggagctga acttgctatg tcactatctg gtcttgaagg tcgtggttat
     1561 atatatatag ttgaaccaac aggaccattt gaagatgatc caaatcttac aaataaaaaa
     1621 tttcctggta atccaacaca atcttataga acttgtgaac ctttaagaat tgttggtgtt
     1681 gttgaagatt gggaaggaca tcctgttgaa ttaataagag gaatgttaga ttcattagaa
     1741 gatttaaaac gtcgtggttt acatgttatt gaagattaac ctggcgcgcc aaataatgcc
     1801 cgtgtccctg ttgaataggg acacggtttt ttaacaattt ttttattcct aggccttagt
     1861 ggccggtacc cggggatcct tattactggc cgctttctag cctggatact tgtctggcta
     1921 gttgcattag agagtcagct agagccctag ggctagctgt ttgtgtattc ggagccagtg
     1981 tagtttttct ttttactaac gatacgggta ataattgatt gccttttaca gcctgacctt
     2041 gagacaattg caaaagtctg tccacagagg tttgacctaa taatctaaaa tcttgtttga
     2101 tagtagtaag tggaggtatg tagcaactag aatcttctgt atcatcataa cccacaactg
     2161 aaatatcagc cccaactcta agtccacttt cagtaatagc tcgcatagct cctaaagcca
     2221 tttgatcatt agcaactaac atagcagtag ggacaatgcc ttcatttaac atttgcattg
     2281 tttgctgaaa tcctgacata gcagaccaat ctccttctct ttctgctatt ggttgtattt
     2341 gatttcttgt taaatacttg tgccatcctg ctaatctcaa tctggcactt actgaactta
     2401 agggacctgc aagtagtgca atttgttgat ggcctaatgc aacaaggtgt tcaactccta
     2461 accttgttcc atcttcgtga gaaaaaatta tagagtttat tggggtttgg tccgacacat
     2521 ccaaaaacaa tgctggaaca ttagtacatg ctgcttcaac tgcaatcgca tcttggtcat
     2581 caagaggata attaattata agtccactaa cacgttgagc taataaatta tgtactgctg
     2641 ccttacaagc ctctacacct gatctctcaa ccattgatac aacaacgctt gcaccaagtt
     2701 gatcagctct cgatttaatt gcggctacaa tttgactagg cgcatgtaat gcaagtgatg
     2761 atgtggcaac tccaattaaa agtgattgtt tccccgcaag ttgctgagca acgcgatttg
     2821 gtatataatt caactctgcc atagctgctt caaccttctc tcttgttttt gcagatacat
     2881 gacttgcttg gttcacaact ctcgaaaccg tttggtagct aaccccggca tattcagcaa
     2941 catcatacaa agtaacaggt ttcatggatc attcctccat gataaaattt aaatttctga
     3001 ctttggcaaa tccttttgcc tgttaaaaag aatagcacaa aaattaaaaa aaaagaaacg
     3061 caaatagcaa tagcattaaa aataattatt attatttatt tctttgatta aaatcaaaag
     3121 cctttaatgt attttcttga tctttcatgc gcttaacggt aaaatccaag gtattaagtt
     3181 caccttccac ttttctctct ctatcttctc tagaaaatca taaaaaattt atttgctttg
     3241 tgagcggata acaattataa tagattcaat tgtgagcgga taacaatttc acacagaatt
     3301 cattaaagag gagaaattac atatggataa gaaatactca ataggcttag ctatcggcac
     3361 aaatagcgtc ggatgggcgg tgatcactga tgaatataag gttccgtcta aaaagttcaa
     3421 ggttctggga aatacagacc gccacagtat caaaaaaaat cttatagggg ctcttttatt
     3481 tgacagtgga gagacagcgg aagcgactcg tctcaaacgg acagctcgta gaaggtatac
     3541 acgtcggaag aatcgtattt gttatctaca ggagattttt tcaaatgaga tggcgaaagt
     3601 agatgatagt ttctttcatc gacttgaaga gtcttttttg gtggaagaag acaagaagca
     3661 tgaacgtcat cctatttttg gaaatatagt agatgaagtt gcttatcatg agaaatatcc
     3721 aactatctat catctgcgaa aaaaattggt agattctact gataaagcgg atttgcgctt
     3781 aatctatttg gccttagcgc atatgattaa gtttcgtggt cattttttga ttgagggaga
     3841 tttaaatcct gataatagtg atgtggacaa actatttatc cagttggtac aaacctacaa
     3901 tcaattattt gaagaaaacc ctattaacgc aagtggagta gatgctaaag cgattctttc
     3961 tgcacgattg agtaaatcaa gacgattaga aaatctcatt gctcagctcc ccggtgagaa
     4021 gaaaaatggc ttatttggga atctcattgc tttgtcattg ggtttgaccc ctaattttaa
     4081 atcaaatttt gatttggcag aagatgctaa attacagctt tcaaaagata cttacgatga
     4141 tgatttagat aatttattgg cgcaaattgg agatcaatat gctgatttgt ttttggcagc
     4201 taagaattta tcagatgcta ttttactttc agatatccta agagtaaata ctgaaataac
     4261 taaggctccc ctatcagctt caatgattaa acgctacgat gaacatcatc aagacttgac
     4321 tcttttaaaa gctttagttc gacaacaact tccagaaaag tataaagaaa tcttttttga
     4381 tcaatcaaaa aacggatatg caggttatat tgatggggga gctagccaag aagaatttta
     4441 taaatttatc aaaccaattt tagaaaaaat ggatggtact gaggaattat tggtgaaact
     4501 aaatcgtgaa gatttgctgc gcaagcaacg gacctttgac aacggctcta ttccccatca
     4561 aattcacttg ggtgagctgc atgctatttt gagaagacaa gaagactttt atccattttt
     4621 aaaagacaat cgtgagaaga ttgaaaaaat cttgactttt cgaattcctt attatgttgg
     4681 tccattggcg cgtggcaata gtcgttttgc atggatgact cggaagtctg aagaaacaat
     4741 taccccatgg aattttgaag aagttgtcga taaaggtgct tcagctcaat catttattga
     4801 acgcatgaca aactttgata aaaatcttcc aaatgaaaaa gtactaccaa aacatagttt
     4861 gctttatgag tattttacgg tttataacga attgacaaag gtcaaatatg ttactgaagg
     4921 aatgcgaaaa ccagcatttc tttcaggtga acagaagaaa gccattgttg atttactctt
     4981 caaaacaaat cgaaaagtaa ccgttaagca attaaaagaa gattatttca aaaaaataga
     5041 atgttttgat agtgttgaaa tttcaggagt tgaagataga tttaatgctt cattaggtac
     5101 ctaccatgat ttgctaaaaa ttattaaaga taaagatttt ttggataatg aagaaaatga
     5161 agatatctta gaggatattg ttttaacatt gaccttattt gaagataggg agatgattga
     5221 ggaaagactt aaaacatatg ctcacctctt tgatgataag gtgatgaaac agcttaaacg
     5281 tcgccgttat actggttggg gacgtttgtc tcgaaaattg attaatggta ttagggataa
     5341 gcaatctggc aaaacaatat tagatttttt gaaatcagat ggttttgcca atcgcaattt
     5401 tatgcagctg atccatgatg atagtttgac atttaaagaa gacattcaaa aagcacaagt
     5461 gtctggacaa ggcgatagtt tacatgaaca tattgcaaat ttagctggta gccctgctat
     5521 taaaaaaggt attttacaga ctgtaaaagt tgttgatgaa ttggtcaaag taatggggcg
     5581 gcataagcca gaaaatatcg ttattgaaat ggcacgtgaa aatcagacaa ctcaaaaggg
     5641 ccagaaaaat tcgcgagagc gtatgaaacg aatcgaagaa ggtatcaaag aattaggaag
     5701 tcagattctt aaagagcatc ctgttgaaaa tactcaattg caaaatgaaa agctctatct
     5761 ctattatctc caaaatggaa gagacatgta tgtggaccaa gaattagata ttaatcgttt
     5821 aagtgattat gatgtcgatg ccattgttcc acaaagtttc cttaaagacg attcaataga
     5881 caataaggtc ttaacgcgtt ctgataaaaa tcgtggtaaa tcggataacg ttccaagtga
     5941 agaagtagtc aaaaagatga aaaactattg gagacaactt ctaaacgcca agttaatcac
     6001 tcaacgtaag tttgataatt taacgaaagc tgaacgtgga ggtttgagtg aacttgataa
     6061 agctggtttt atcaaacgcc aattggttga aactcgccaa atcactaagc atgtggcaca
     6121 aattttggat agtcgcatga atactaaata cgatgaaaat gataaactta ttcgagaggt
     6181 taaagtgatt accttaaaat ctaaattagt ttctgacttc cgaaaagatt tccaattcta
     6241 taaagtacgt gagattaaca attaccatca tgcccatgat gcgtatctaa atgccgtcgt
     6301 tggaactgct ttgattaaga aatatccaaa acttgaatcg gagtttgtct atggtgatta
     6361 taaagtttat gatgttcgta aaatgattgc taagtctgag caagaaatag gcaaagcaac
     6421 cgcaaaatat ttcttttact ctaatatcat gaacttcttc aaaacagaaa ttacacttgc
     6481 aaatggagag attcgcaaac gccctctaat cgaaactaat ggggaaactg gagaaattgt
     6541 ctgggataaa gggcgagatt ttgccacagt gcgcaaagta ttgtccatgc cccaagtcaa
     6601 tattgtcaag aaaacagaag tccagacagg cggattctcc aaggagtcaa ttttaccaaa
     6661 aagaaattcg gacaagctta ttgctcgtaa aaaagactgg gatccaaaaa aatatggtgg
     6721 ttttgatagt ccaacggtag cttattcagt cctagtggtt gctaaggtgg aaaaagggaa
     6781 atcgaagaag ttaaaatccg ttaaagagtt actagggatc acaattctgg aaagaagttc
     6841 ctttgaaaaa aatccgattg actttttaga agctaaagga tataaggaag ttaaaaaaga
     6901 cttaatcatt aaactaccta aatatagtct ttttgagtta gaaaacggtc gtaaacggat
     6961 gctggctagt gccggagaat tacaaaaagg aaatgagctg gctctgccaa gcaaatatgt
     7021 gaatttttta tatttagcta gtcattatga aaagttgaag ggtagtccag aagataacga
     7081 acaaaaacaa ttgtttgtgg agcagcataa gcattattta gatgagatta ttgagcaaat
     7141 cagtgaattt tctaagcgtg ttattttagc agatgccaat ttagataaag ttcttagtgc
     7201 atataacaaa catagagaca aaccaatacg tgaacaagca gaaaatatta ttcatttatt
     7261 tacgttgacg aatcttggag ctcccgctgc ttttaaatat tttgatacaa caattgatcg
     7321 taaacgatat acgtctacaa aagaagtttt agatgccact cttatccatc aatccatcac
     7381 tggtctttat gaaacacgca ttgatttgag tcagctagga ggtgactaat aaaagcttga
     7441 tcagatccca gctttttttt gaagtgcctg gcagtaagtt gaaaaaattt gaaataaact
     7501 tggactatgt taataaaaaa atacaaatta acgaacaaaa agatttgaat tattatataa
     7561 tttagttaaa agcaatttta aatgagggag gtttccatat gggccatatt caacgggaaa
     7621 cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc
     7681 gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc
     7741 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg
     7801 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta
     7861 ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat
     7921 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc
     7981 ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg
     8041 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc
     8101 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac
     8161 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga
     8221 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg
     8281 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa
     8341 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt
     8401 ttttctaatg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcatga
     8461 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     8521 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     8581 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     8641 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     8701 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     8761 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     8821 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     8881 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     8941 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     9001 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     9061 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     9121 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     9181 tctttcctgc actagatccc cggcaagttc atccttaggg ttttcatatc tcttaaaaag
     9241 tcaatttctg cttgattaat ttcttgtgga ttattgtttt tgcggttttt cattttttat
     9301 ctccttgagt attgaatttt ttcttatata taaatatata gcaaaaacta tttttgccaa
     9361 cttttttaca aaaaattttt tattaaaaca cttagggctt tgctaaattc tcttttaaaa
     9421 gaacttagta aagccctatg gatttaagaa ctgattatac ttacgtagta aaaatactac
     9481 agatattaat agtatagacc atatcaaaat cataatcaat ttatatttat ctaaaattgt
     9541 agtccataca aaatacaaaa aaattaggtt tttctaaatt ctattttaaa agaacttagt
     9601 aacacctaat ctctaaatta cttacaaaat tctaaaatta gatttttagt tcttcatatt
     9661 tctttaaaag ttttttaaga aaatcttttt gattttcaaa aatttcatgc atcataaaac
     9721 tagtaaattt ggattttttt ttgtaaaaat cataactttc ttgggtttta agttgaaatc
     9781 ttaatggttt tattggattt tgcctagatt tttttaacgc cggactttct ttatctttta
     9841 taaaattata agaattttta ataccatttt ctattaaata atcttctttt ataagcccct
     9901 cttgcagtgc agttgctaat cttaaatact tatacgcttg agtttttgct acatcaaagt
     9961 cttttataaa atcaagaaaa gttttataac catcaaattt ataatattga ttatctttta
    10021 tttcttttaa aattttaata ttttccaccc tgtggaatat ttcttttttg aaattgtcat
    10081 ttaacttttc ctttagtgtc ttgtaataag aaattttact ttcattaatc attaaatttt
    10141 gttcaacata agtatctatc ttttttgtta aaactttaac aacacgtttt ttttccataa
    10201 aaattttcct ttatttttgc aacattttcc ttaattgata taattgttac tattttagat
    10261 tttttaatat ccacttaaga attaatccct cactagaata ttttttatta tagaattcat
    10321 atacatgttt ttctaaatct ataaatttat caacaattgc tttattatag ggtgtatcta
    10381 tcttttcttt tcttaataat cgtctcatat tctttaaata acaacaaaca ctgccggttt
    10441 taaacctaaa ttctatgtaa tatatttttg aaaaagtata tacattttca accccgttta
    10501 tttcatactt tacaaaaaca ttatttattg gttttctata accatagtaa atgcctaaaa
    10561 atttgtcccc gtctttcata ggatatagat aaagtttttg aatctctgat ttatctaata
    10621 atttttcaaa aaaaattata caccgctttt gtttgttact aactactcta tgcaatgcct
    10681 ttaatatttt tgtgtgatat atatttttat cacctttttt ttctttcagc aggaagatac
    10741 tttcttttgg aaatttaatt ttttgttcat ttattcttgc agccttaagt ccgattttca
    10801 tatttaatcc ttatatagcc tttctattta attcttttaa actcaataaa cttttttggt
    10861 ttttcattag ttctaataat tcgcaataat atgtgcaaaa cactttattg tattctattt
    10921 tcttttggtt attcaaatat tcttttataa ttggctttag aagttcaata tttacttctt
    10981 tttttagttg ttcaataagg atattgaaaa tgtttgtttt taaatcttga taattttgtt
    11041 gagaattttc tttttttctt tcaattgact tctctaattt acgctttata ttgtttaaat
    11101 cgctatattt atgactttca ataataaaat gaggcttaaa tttgtaattt tcgtatattt
    11161 tttggaaact tgtttctaat tgtttagaat tatattcatt tttttcaaat tctttttgag
    11221 tgttgcatag aatttccttt aatttttctt gtttttcttt catacaagat ttagtaaaat
    11281 gtatattttt attttttagt aatttaattt ctattatttt tatgatttta agt
//
