LOCUS       sequence-306493-       11554 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast expression of Sad1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11554)
  AUTHORS   Jiang Z, Jin X, Li Y, Liu S, Liu XM, Wang YY, Zhao P, Cai X, Liu Y, 
            Tang Y, Sun X, Liu Y, Hu Y, Li M, Cai G, Qi X, Chen S, Du LL, He W
  TITLE     Genetically encoded tags for direct synthesis of EM-visible gold 
            nanoparticles in cells.
  JOURNAL   Nat Methods. 2020 Sep;17(9):937-946. doi: 10.1038/s41592-020-0911-z.
            Epub 2020 Aug 10.
  PUBMED    32778831
REFERENCE   2  (bases 1 to 11554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-0911-z"; journalName: "Nat Methods"; date: 
            "2020-09"; volume: "17"; issue: "9"; pages: "937-946"
COMMENT     Sequence Label: pDUAL-P41nmt1-Sad1-GFP-MTn
FEATURES             Location/Qualifiers
     source          1..11554
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    288..309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(324..329,330..347,348..354)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    362..378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     367..389
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     386..402
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     386..402
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3175..3888
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      4104..4291
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4159..4183)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(5955..6749)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     rep_origin      7358..8141
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(8864..8881)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8864..8880)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8873..8895)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9093..9548
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9180..9199)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9390..9411
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9565..9584)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9684..9706
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9744..9762)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9830..9934
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9935..10003,10004..10795)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10153..10172)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10966..11554
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11455..11474
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      241 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      301 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      361 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt
      421 gcatgcctgc aggtcgatcg actctagagg atcagaaaat tatcgccata aaagacagaa
      481 taagtcatca gcggttgttt catttcctat attttttttt tattttttta ttttttaata
      541 agggaaaatt taacgtctaa ggatacagaa gattgttagc acattaaagt aataaaggct
      601 taagtagtaa gtgccttagc atgttattgt atttcaaagg acataatcta aaataataac
      661 aatatcattt ctcacaagtt attcaatttt cttttttttt tctaataata tcaagaatgt
      721 attatttgtt tgacataagt caactaattt atttaatatg ctggattaat cttgcagaca
      781 tgtaaattaa caagttttag tcaaataacg ttgaagtttc aatgaactca aataatttct
      841 cttttttttt atataaccat aatctgattt atattttccg cagggatcaa ctgaagttat
      901 gacatttgga ttggatcact tataaccttg gtcgccaaat aatacaaaaa tcagcgttat
      961 aaaacaaaga aggtttttgt taagaaatta atcctctttc ttgataagaa agttgaaccg
     1021 aaattgcaga tactgatata tgaaaataat acccacaatt ttgggaatag cgcaagcctc
     1081 aatttaaaca ataggtgagg acacatgata atgacctcaa tgattgttag aagaaaagag
     1141 cctcattaca aaatcgaaaa atgaatggtt gggtacaagt ttccaaaaca tggtaaagtg
     1201 gactttgcgt atgagacgta aatagaaaaa aacacttgtt atatgttttc tagaattatt
     1261 gttgtctctt tatggttgga tgatgcaaaa tagtaatttc ggttagttgc tgtaaaacac
     1321 cacgagacaa atagatatgg atatttatta aatcaggaaa aacgtaactc tcggctactg
     1381 gatggttcag tcacccaacg attactgggg agagaaaaca gggcaaaagc aaagcttaaa
     1441 ggaatccgat tgtcattcgg caatgtgcag cgaaactaaa aaccggataa tggacctgtt
     1501 aatcgaaaca ttgaagataa aggaagagga atcctggcat atcatcaatt gaataagttg
     1561 aattaattat ttcaatctca ttctcacttt ctgacttata gtcgctttgt taaagctagc
     1621 ggatccatgt ttactaatac accggttgga gggaaaagag agcgtcaaaa tggcgctcat
     1681 ccagcttggt cgacgttagg agcgaactct gctcagattc atcaaaatac ggctgatttg
     1741 gcctccaaaa tgcataaact tcgctataca aagataaggt cccctcctac cagggtgtcc
     1801 attgaatcaa ttactcctaa acaaagattt ccagctccca atttcgaaca ggcttaccac
     1861 tctaatattc gatacgagca agaggaatct gataacgaag aatttgaaaa cgtagtgaaa
     1921 aatggacacg aagcttcgac gaatgtattc tatgaatcag atggagatga tgaagagttt
     1981 gtcaatgaag aatacgaaaa cagcatcgac gaggaaagtg atgatgaagg gtatagcttg
     2041 aacgaggata ccaccgcaac aaatgccagt tttaggtacc cgatgaatca aagatctaca
     2101 aggaaatctc aattttattc ctcaaaattt aaaccattac tttggttcgg tatcactctc
     2161 ttttcgactc tcctaataat tactctactt cacaagggtc aagaatttta ctcaagatca
     2221 ttttcatcag ataattccca accatctaat tcgcccgtac caaacattcc tcctgcatcc
     2281 aatgacacca aaacaagttt aaagccagat attattaaag acttcacaga ttcaccttct
     2341 aaagttggcg gcaatgagga attcgattat tcgacgggag atttaattac aaaaaaggaa
     2401 tttgacaaga ttttacaaca aaaagtagag caattaaaac agtcgctaaa ggaggagatg
     2461 tctaattaca aatcatcggt cccatttgaa gttgaactaa atgacgattg gaagttcttc
     2521 atcgaatcta cggttcgaaa atacttgacc gatccggttt ccatgccgaa ttttgcattg
     2581 ctctcaacag gagcggaagt actacctgcc ttaacctcaa aacgctatgt gagacgacca
     2641 tctgcattca taccacgatt tacctcctat ttctttgatt cgctcgttgt tagaggacac
     2701 gaacctagca tagcattaac tcccaacaac gcagttgcga tgtgttggag ctttcaaggt
     2761 tcagagggac aattaggaat ttccctatca cgccctgtat atgtaaccaa tgtcacaata
     2821 gagcacgttc agcacaagat tgctcatgat ctttctagcg ctcccaagga ctttgagttg
     2881 tgggttcagg gaatgtccag caagatgttt gtattgttgg gaaaggcgag atattcttta
     2941 acggaagact ctattcaaac tttttcgttt gaatcctcca attatattgt cgctgaaccg
     3001 atacagaatg tgatcttaaa aataaaaagc aactggggaa atcccaatta cacatgcttg
     3061 tatcaagttc gagttcatgg gactgtaccc aatgcagacg aacaaccaat accttcttta
     3121 ggtgagaaag ccgaaagtac tgcagagaat acgggtcaag attcatctgg atccatgtct
     3181 aaaggtgaag aattattcac tggtgttgtc ccaattttgg ttgaattaga tggtgatgtt
     3241 aatggtcaca aattttctgt ctccggtgaa ggtgaaggtg atgctactta cggtaaattg
     3301 accttaaaat ttatttgtac tactggtaaa ttgccagttc catggccaac cttagtcact
     3361 actttcggtt atggtgttca atgttttgcg agatacccag atcatatgaa acaacatgac
     3421 tttttcaagt ctgccatgcc agaaggttat gttcaagaaa gaactatttt tttcaaagat
     3481 gacggtaact acaagaccag agctgaagtc aagtttgaag gtgatacctt agttaataga
     3541 atcgaattaa aaggtattga ttttaaagaa gatggtaaca ttttaggtca caaattggaa
     3601 tacaactata actctcacaa tgtttacatc atggctgaca aacaaaagaa tggtatcaaa
     3661 gttaacttca aaattagaca caacattgaa gatggttctg ttcaattagc tgaccattat
     3721 caacaaaata ctccaattgg tgatggtcca gtcttgttac cagacaacca ttacttatcc
     3781 actcaatctg ccttatccaa agatccaaac gaaaagagag accacatggt cttgttagaa
     3841 tttgttactg ctgctggtat tacccatggt atggatgaat tgtacaaaag atctatggac
     3901 cccgcctgtg cgtgtgcagc cggcggcgct tgcgcttgcg caggcgcttg cgcctgcgca
     3961 gcatgcgcat gtgccgcttg cgcagcaagc tgctgcgcct gctgtcccgt gggctgcgcc
     4021 gcatgtgccg caggctgtgt ctgcgcaggc gccgcggacg cgtgcgcgtg ctgtgccaga
     4081 tctggcgcgc cacttctaaa taagcgaatt tcttatgatt tatgattttt attattaaat
     4141 aagttataaa aaaaataagt gtatacaaat tttaaagtga ctcttaggtt ttaaaacgaa
     4201 aattcttatt cttgagtaac tctttcctgt aggtcaggtt gctttctcag gtatagtatg
     4261 aggtcgctct tattgaccac acctctaccg gcagatccgc tagggataac agggtaatat
     4321 actagtaaga tgattctttt atcgtcgata acgatgagga tgtagacgat tatgatgaga
     4381 gtgattagct tctaaaattt ggtgttttca aacgctattt acagtacaat ctgaaaaaat
     4441 tggatgaaca ttgtaaatgg taggatacga gttccaatta atgtattttt taagtattac
     4501 acttagctta ttaattttat atatacattc tacctctgtg ggtttaaaga caactcgtac
     4561 acatactcaa attatttatg aacattataa tgcatattcg tactgtctat taaacatttt
     4621 tttgacatta aaaatttcgt ttactaacgt agaaagcgct gataattgag gctactattt
     4681 taatatctag aaacaaagat tcattaatta attaagtaag ctccaggata cttgtatatt
     4741 tcgttaaaaa agcgcaattt caacaattcc tatgaacatc catgatttca tcacaaaatc
     4801 aaacatatca ggatttaacg tgaattattt gctttcttgt gattttcatc tgatttctgg
     4861 tcatttacgt tactgtaaac aagcttttta aaaaaaggat tttttacgca atattatcga
     4921 tatcccaatc tgtagtataa aaagtgcaac agtgttgagt ttcccgaaac caggcatttt
     4981 tgcggtaggg tctgaaactt tcacgaaacc atttcacaac aaaacttctt caacaacatg
     5041 tgtgcaaaga aaatcgtcgt cttaccagga gaccatattg gccctgaaat tgttgcttct
     5101 gccttggagg ttttgaaagt cgttgagaag aagcgacctg agttaaaact cgagtttgaa
     5161 gaacacaaga ttggaggtgc ctctattgat gcctatggaa cccctttgac tgacgagact
     5221 gtgaaggctt gtttggaagc tgacggtgtt cttttgggtg ccgttggtgg tcctgaatgg
     5281 accaacccca attgtcgtcc tgagcaaggt ttattgaagc ttcgtaagag tatgggtgtt
     5341 tgggccaacc ttcgaccttg caactttgcc agcaagtctt tagtcaagta cagccctttg
     5401 aagcctgaaa tcgttgaagg tgtcgatttt tgtgttgtac gagaacttac tggaggttgt
     5461 tactttggtg agcgcactga ggacaacgga tcgggttatg ctatggacac ttggccttac
     5521 agtttggaag aagtttctcg tattgctcgt ttggcggccg cgggcccaac accaatgttt
     5581 ataaccaagt tttatcttgt ttgtctacat ggtattttac attcatctac atacatcttt
     5641 cattggcttt gtacatagtt atcattacaa gtctaaaaaa attcactctt ttcttattca
     5701 atgtcaatcc aagagaaaag attgtggtaa tgttgtagga gcatgtttaa taaattacta
     5761 tagcaaatta ctttttattc ccaaggtgtt tatctataat agttaatatt ttagtcgcta
     5821 cataaaattt taccaaagag tacttgtata ctaattctaa atgccttctg acataaaacg
     5881 cctaggaaaa caaacgcaaa caaggcatcg actttttcaa taaccaacca aaaaaatttt
     5941 acattagtct ttttttaatg ctgagaaagt ctttgctgat atgccttcca accagcttct
     6001 ctatatctct tggcttcgac aacaggatta cgaccagctc catagactcc acgaccaaca
     6061 atgatgatgt cgctaccgca gtttacaatc acttcttcag gagtacgata ttgctgtccc
     6121 agcccgtctc ctttaacatc caagccgata ccaggggaca tagttatgta gtcgctttga
     6181 aggttaggaa atcgacgacc agctataaag ccaaagcaaa aatcggtatg cttctcaaac
     6241 cattctaagg ttttctctgt gtaggaacca gtagccaaag agcctttgga agacatttca
     6301 gccaaaagca agagaccacg tcccaaaggt aaaccaactt ctttgaggcc ttgtataata
     6361 ccctcgcctg gcactgtatg gcaatttgtg atatgagccc aagaagcaat tttgtacaca
     6421 ccagatgcat attgtagctt gacggtattt ccaatgtctg cgaatttgcg atcctcaaag
     6481 ataagaaaac gatgcttttt acctaaggcc accagttttt ctaccatatc ctggtcgaaa
     6541 tcctcgacaa cgtcaatatg tgtcttgata acacagacat agggtccaat tttatctacc
     6601 aattctaaga tttcggattt cttcgtcaaa tcgaccgcga ctgacaagtt gctttgcttt
     6661 tcttccatca aagccaacaa ttccttggca atgggatttt tcatcccctc agctctagct
     6721 gaatagcttt gaaatactct agcatccata actttgcttt taaaccttta atttcgatcc
     6781 aagcaaaaaa gaggttcttg gtaggacaat acggtaagaa aacacgacat gtgcagagat
     6841 gccgacgaag catagttaaa ctgggatggt aaaatcaatt aagaatttat aaagacaaaa
     6901 ttgtataagt ctctaaaaca tcttaattat acctcacaga actatctaaa atatattcac
     6961 aaagtgcaaa cattatcatg aaaaagaacc attttaattt aaagcaaggg cattaaggct
     7021 tatttacaga atttcttact tttgtaaaga ttataaggct gattatcttt ttcaccatgc
     7081 caaaaattac acaagataga atggatgttt gaaattaaac gtgagtatac aaacaaatac
     7141 actaggtaaa tcgaaacatt tttttctcca ttaagtaaca aattcctatt tagagaaaga
     7201 atgctgagta gattaaataa tctatacaaa cttttttaac acaaatgcat acatatagcc
     7261 agtgggattt gtagctaagc ttgcgaattc gagtctaact ccttaaccac tcggacatag
     7321 tgacttatct gacactcatt gtaaattaat atatatatag gcattttgtt tagttaaagg
     7381 tacttaagta attagtataa acgaaccaat tttataatca ggaagttaag tgaatggtag
     7441 cacatgtcgt aaaaattgtg aatttttatt gaataatatt ttaaatacaa gcctttctag
     7501 actaggtata ctcataaaca tatatgagca aaaggataga ggagattaca ttgcatcttc
     7561 tacaaattta tttattgccc tttactgaaa aattaaataa tgagtactaa atgataaaaa
     7621 gcgctcagta caagaaagat tgcaaaaata ttgcattctt catgaattaa agttgcatat
     7681 aaggcatatt gaaagtaata gtactaaaac agcagttagc gaaaattaat agaattatat
     7741 tcgcaagaca attgtgacat taaattaaaa aattgtagaa tttttactat cctctttaac
     7801 gccatgagcc tttataaaaa ggttaaatta gttttaacat tctttttttg agtaagagtt
     7861 tacaatttat caaaacctgt gttattatat tcattagttt caatttatta gcatctagag
     7921 aaaaatcaat tggcagttac attgttggaa tttatgaaga aaaagaatct acaacggaga
     7981 ataagttgct gatcgctttc cctaaaattg tatattttgc tgagcttatt ttgacatttc
     8041 gttgaagttt tctctaattc gcattcattt taagtaaaac aatgagaaat aaaattacaa
     8101 aaaatacaaa ttaaaataca atttttagct ataattatag acgatgccct tgtatcccat
     8161 tctgtctcgc ttgcccctac tttttatctt ttatatacca taatgaacgc tgccgctact
     8221 aaccataccc cgattttaca tttcggactc ccaaggacgt acaaaataga aaactataga
     8281 aaaaaataat cagaaaatag catgtcatct ctttgtaaaa cgcgtttgca agaagaaagg
     8341 aaacaatgga gaagagatca tccatttgta tgtaaatttt agtaaacttg aagaaatcac
     8401 taacaacttc tcttacttag ggattctatg caaaaccttg taaatcatct gatggaggac
     8461 tcgatttaat gaattggaag gttgggcccg cggccgctta gctgaaactt ccaaccctcc
     8521 tgctcccgtc acattactcg acaaagctaa tgttttggca acttctcgtc tttggcgcaa
     8581 gactgtagct aagattttta aggaggaata tcctcacctt accttaaaaa accagctcat
     8641 tgactccgca gccatgcttt tggtcaagag ccctcgtaca cttaacggtg ttgttttgac
     8701 tgacaacttg tttggtgaca ttatttcaga tgaggcttct gtcattcctg gtagcttggg
     8761 ccttttgcct tctgcctccc tttccggtgt ggtaggaaaa tcagaagaaa aggttcattg
     8821 tttggttgag cccattcacg gtagcgctcc cgagctcgaa ttcactggcc gtcgttttac
     8881 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     8941 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     9001 gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     9061 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
     9121 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc
     9181 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     9241 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     9301 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     9361 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     9421 caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg
     9481 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     9541 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     9601 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     9661 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     9721 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     9781 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     9841 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     9901 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     9961 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
    10021 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
    10081 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
    10141 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
    10201 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
    10261 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
    10321 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
    10381 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
    10441 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
    10501 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
    10561 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
    10621 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
    10681 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
    10741 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
    10801 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
    10861 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
    10921 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
    10981 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
    11041 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
    11101 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
    11161 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
    11221 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
    11281 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
    11341 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    11401 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    11461 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    11521 gtgatgctcg tcaggggggc ggagcctatg gaaa
//
