LOCUS       sequence-306494-       10922 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses iRFP713, PuroR and rtTAM2 in mammalian cells, with a TRE 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Yoshimatsu S, Nakamura M, Nakajima M, Nemoto A, Sato T, Sasaki E, 
            Shiozawa S, Okano H
  TITLE     Evaluating the efficacy of small molecules for neural 
            differentiation of common marmoset ESCs and iPSCs.
  JOURNAL   Neurosci Res. 2019 Oct 3. pii: S0168-0102(19)30262-7. doi: 
            10.1016/j.neures.2019.09.005.
  PUBMED    31586586
REFERENCE   2  (bases 1 to 10922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neurosci 
            Res. 2019 Oct 3. pii: S0168-0102(19)30262-7. doi: 
            10.1016/j.neures.2019.09.005."
COMMENT     Sequence Label: pPB-tetpA-iRPT
FEATURES             Location/Qualifiers
     source          1..10922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(180..304)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(314..628)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(317..341)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    388..406
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    424..442
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    459..477
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    495..513
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    531..549
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    566..584
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    602..620
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        665..1827
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          896..1818
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1775..1795
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1840..1849
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2794..2847
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2854..3450
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2854..2873)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3350..3370
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3451..3507
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(3508..4125,4126..4131,4132..4167,4168..4170,4171..4206,
                     4207..4209,4210..4245,4246..4254)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    4304..4892
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4357..4377)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4775..4786)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5178..5299
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5215..5234)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5269..5288
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   5488..5550
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(5574..5592)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5613..5629)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5613..5629)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5626..5648)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5637..5653
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5661..5667,5668..5685,5686..5691))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5706..5727
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5844..5861)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6015..6603)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6095..6114)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6774..7565,7566..7634))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7397..7416
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7635..7739)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7765..8220)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7902..7923)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8114..8133
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8347..8369
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8361..8378
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8362..8378
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8385..8404
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8385..8403
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   8422..8456
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    8731..8938
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    8960..9084
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        9262..9364
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             9365..10048
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     complement(9431..9450)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             10368..10673
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     10552..10571
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tttcttttta tgatttaata cggcattgag gacaatagcg agtaggctgg atacgacgat
       61 tccgtttgag aagaacattt ggaaggctgt cggtcgacta agttggcagc atcacccgaa
      121 gaacatttgg aaggctgtcg gtcgactaca ggtcactaat accatctaag tagttgattc
      181 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct
      241 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttt ttgtacaaac
      301 ttgtgatgct agcggcgatc tgacggttca ctaaacgagc tctgcttata taggcctccc
      361 accgtacacg cctacctcga catacgttct ctatcactga tagggagtaa actcgacata
      421 cgttctctat cactgatagg gataaactcg acatacgttc tctatcactg atagggagta
      481 aactcgacat acgttctcta tcactgatag ggagtaaact cgacatacgt tctctatcac
      541 tgatagggag taaactcgac atcgttctct atcactgata gggagtaaac tcgacatacg
      601 ttctctatca ctgataggga gtaaactcga ttgaagacga aagggccgaa ttcaagcttc
      661 gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
      721 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
      781 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
      841 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
      901 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
      961 aattacttcc acctggctcc agtacgtgat tcttgatccc gagctggagc caggggcggg
     1021 ccttgcgctt taggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg
     1081 ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct
     1141 ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt
     1201 gtaaatgcgg gccaggatct gcacactggt atttcggttt ttggggccgc gggcggcgac
     1261 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg
     1321 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
     1381 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg
     1441 gaaagatggc cgcttcccgg ccctgctcca gggggctcaa aatggaggac gcggcgctcg
     1501 ggagagcggg cgggtgagtc acccacacaa aggaaagggg cctttccgtc ctcagccgtc
     1561 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctggagc
     1621 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
     1681 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
     1741 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
     1801 ttttttcttc catttcaggt gtcgtgaaca cgctaccggg ccaccatggc cgaagggagc
     1861 gtggcaagac agcccgatct gctgacatgc gacgacgaac caatccacat tccaggagct
     1921 atccagcctc atggactgct gctggctctg gccgctgaca tgactatcgt ggcaggctct
     1981 gataacctgc cagagctgac cgggctggct atcggagcac tgattggccg gtccgcagcc
     2041 gacgtgttcg attctgagac tcacaataga ctgaccatcg ccctggctga acctggggct
     2101 gcagtgggag ccccaattac cgtcggcttc acaatgcgaa aggacgctgg ctttatcggg
     2161 tcctggcacc gccatgatca gctgattttt ctggagctgg aaccacctca gagagacgtg
     2221 gcagagccac aggctttctt tcggagaaca aacagcgcca tcaggcgcct gcaggccgct
     2281 gagactctgg aatccgcttg cgcagccgct gcacaggaag tgaggaagat caccgggttc
     2341 gatcgcgtca tgatctacag attcgcctca gactttagcg gagaagtgat cgcagaagat
     2401 cgctgtgccg aggtcgaaag taaactgggc ctgcattacc cagcctcaac cgtgcctgca
     2461 caggcacgac ggctgtatac aatcaacccc gtgcggatca ttcccgacat taattacaga
     2521 ccagtgcccg tcacacctga cctgaaccca gtgactggga ggcccatcga tctgagcttc
     2581 gccattctgc gcagcgtgtc ccctgtccac ctggagttta tgaggaatat cggaatgcat
     2641 ggcaccatgt ctatcagtat tctgagaggc gaaaggctgt gggggctgat cgtgtgccac
     2701 catcggacac cttactatgt ggacctggac ggcaggcagg cttgtgaact ggtggctcag
     2761 gtgctggctt ggcagattgg cgtgatggaa gaagagggca gaggaagtct tctaacatgc
     2821 ggtgacgtgg aggagaatcc cggccctatg tctatgaccg agtacaagcc cacggtgcgc
     2881 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     2941 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     3001 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     3061 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     3121 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     3181 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     3241 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     3301 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     3361 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     3421 acctggtgca tgacccgcaa gcccggtgcc gccacgaact tctctctgtt aaagcaagca
     3481 ggagacgtgg aagaaaaccc cggtcctatg tctagactgg acaagagcaa agtcataaac
     3541 ggcgctctgg aattactcaa tggagtcggt atcgaaggcc tgacgacaag gaaactcgct
     3601 caaaagctgg gagttgagca gcctaccctg tactggcacg tgaagaacaa gcgggccctg
     3661 ctcgatgccc tgccaatcga gatgctggac aggcatcata cccacttctg ccccctggaa
     3721 ggcgagtcat ggcaagactt tctgcggaac aacgccaagt cattccgctg tgctctcctc
     3781 tcacatcgcg acggggctaa agtgcatctc ggcacccgcc caacagagaa acagtacgaa
     3841 accctggaaa atcagctcgc gttcctgtgt cagcaaggct tctccctgga gaacgcactg
     3901 tacgctctgt ccgccgtggg ccactttaca ctgggctgcg tattggagga acaggagcat
     3961 caagtagcaa aagaggaaag agagacacct accaccgatt ctatgccccc acttctgaga
     4021 caagcaattg agctgttcga ccggcaggga gccgaacctg ccttcctttt cggcctggaa
     4081 ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa gcggcgggcc ggccgacgcc
     4141 cttgacgatt ttgacttaga catgctccca gccgatgccc ttgacgactt tgaccttgat
     4201 atgctgcctg ctgacgctct tgacgatttt gaccttgaca tgctccccgg gtaactaagt
     4261 aaggatcctc tagagttaac atcgagggat caagcttatc gataatcaac ctctggatta
     4321 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     4381 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     4441 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     4501 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     4561 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     4621 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     4681 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     4741 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     4801 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     4861 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgagacc tagaaaaaca
     4921 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     4981 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     5041 ttacaaggca gctgtagatc tctagataac tgatcataat cagccatacc acatttgtag
     5101 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     5161 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5221 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5281 aactcatcaa tgtatcttaa cgctcgagtg catggatcct ttgttacttt atagaagaaa
     5341 ttttgagttt ttgttttttt ttaataaata aataaacata aataaattgt ttgttgaatt
     5401 tattattagt atgtaagtgt aaatataata aaacttaata tctattcaaa ttaataaata
     5461 aacctcgata tacagaccga taaaacacat gcgtcaattt tacgcatgat tatctttaac
     5521 gtacgtcaca atatgattat ctttctaggg ttaaagagct ccagcttttg ttccctttag
     5581 tgagggttaa tttcgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     5641 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     5701 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     5761 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     5821 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     5881 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     5941 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6001 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6061 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6121 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6181 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     6241 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6301 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     6361 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     6421 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     6481 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     6541 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6601 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6661 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     6721 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     6781 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     6841 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     6901 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     6961 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     7021 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     7081 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7141 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     7201 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7261 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7321 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     7381 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     7441 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     7501 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     7561 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     7621 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     7681 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     7741 acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt
     7801 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     7861 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     7921 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     7981 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     8041 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     8101 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     8161 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     8221 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     8281 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     8341 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat
     8401 agggcgaatt gggtacctta accctagaaa gatagtctgc gtaaaattga cgcatgcatt
     8461 cttgaaatat tgctctctct ttctaaatag cgcgaatccg tcgctgtgca tttaggacat
     8521 ctcagtcgcc gcttggagct cccgtgaggc gtgcttgtca atgcggtaag tgtcactgat
     8581 tttgaactat aacgaccgcg tgagtcaaaa tgacgcatga ttatctttta cgtgactttt
     8641 aagatttaac tcatacgata attatattgt tatttcatgt tctacttacg tgataactta
     8701 ttatatatat attttcttgt tatagatatc tccccagcat gcctgctatt gtcttcccaa
     8761 tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca cctactcaga
     8821 caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc accttccagg
     8881 gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga aggcacagat
     8941 cgtcgagacc ggtagcatca ccactttgta caagaaagct gaacgagaaa cgtaaaatga
     9001 tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa
     9061 aacacaacat atccagtcac tatgaatcaa ctacttagat ggtattagtg acctgtagtc
     9121 gactaagttg gcagcatcac ccgacgcact ttgcgccgaa taaatacctg tgacggaaga
     9181 tcacttcgca gaataaataa atcctggtgt ccctgttgat accgggaagc cctgggccaa
     9241 cttttggcga aaatgagacg ttgatcggca cgtaagaggt tccaactttc accataatga
     9301 aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc
     9361 taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa
     9421 agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct
     9481 ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt
     9541 tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga
     9601 cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac
     9661 tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat
     9721 atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat
     9781 tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa
     9841 cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca
     9901 aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt
     9961 ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc agggggggcg
    10021 taaacgccgc gtggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct
    10081 gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt
    10141 atgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg
    10201 catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg
    10261 tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt
    10321 tattgaaatg aacggctctt ttgctgacga gaacaggggc tggtgaaatg cagtttaagg
    10381 tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta
    10441 ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata
    10501 aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga
    10561 ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc
    10621 accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag
    10681 gctcccttat acacagccag tctgcaggtc gatacagtag aaattacaga aactttatca
    10741 cgtttagtaa gtatagaggc tgaaaatcca gatgaagccg aacgacttgt aagagaaaag
    10801 tataagagtt gtgaaattgt tcttgatgca gatgattttc aggactatga cactagcgta
    10861 tatgaatagg tagatgtttt tattttgtca cacaaaaaag aggctcgcac ctctttttct
    10921 ta
//
