LOCUS       sequence-306495-        9560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses iRFP713, PuroR and rtTAM2 in mammalian cells, with 
            TRE-hNEUROG2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9560)
  AUTHORS   Yoshimatsu S, Nakamura M, Nakajima M, Nemoto A, Sato T, Sasaki E, 
            Shiozawa S, Okano H
  TITLE     Evaluating the efficacy of small molecules for neural 
            differentiation of common marmoset ESCs and iPSCs.
  JOURNAL   Neurosci Res. 2019 Oct 3. pii: S0168-0102(19)30262-7. doi: 
            10.1016/j.neures.2019.09.005.
  PUBMED    31586586
REFERENCE   2  (bases 1 to 9560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neurosci 
            Res. 2019 Oct 3. pii: S0168-0102(19)30262-7. doi: 
            10.1016/j.neures.2019.09.005."
COMMENT     Sequence Label: pPB-tetpA-hNEUROG2-iRPT
FEATURES             Location/Qualifiers
     source          1..9560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    36..54
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    72..90
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    107..125
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    143..161
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    179..197
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    214..232
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    250..268
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        313..1475
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          544..1466
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1423..1443
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1488..1497
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2442..2495
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2502..3098
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2502..2521)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2998..3018
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3099..3155
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(3156..3773,3774..3779,3780..3815,3816..3818,3819..3854,
                     3855..3857,3858..3893,3894..3902)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    3952..4540
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4005..4025)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4423..4434)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4826..4947
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4863..4882)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4917..4936
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   5136..5198
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(5222..5240)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5261..5277)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5261..5277)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5274..5296)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5285..5301
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5309..5315,5316..5333,5334..5339))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5354..5375
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5492..5509)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5663..6251)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5743..5762)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6422..7213,7214..7282))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7045..7064
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7283..7387)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7413..7868)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7550..7571)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7762..7781
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7995..8017
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8009..8026
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8010..8026
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8033..8052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8033..8051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   8070..8104
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    8379..8586
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    8608..8632
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9488..9512)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(join(9522..9560,1..276))
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(9525..9549)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ggcctcccac cgtacacgcc tacctcgaca tacgttctct atcactgata gggagtaaac
       61 tcgacatacg ttctctatca ctgataggga taaactcgac atacgttctc tatcactgat
      121 agggagtaaa ctcgacatac gttctctatc actgataggg agtaaactcg acatacgttc
      181 tctatcactg atagggagta aactcgacat cgttctctat cactgatagg gagtaaactc
      241 gacatacgtt ctctatcact gatagggagt aaactcgatt gaagacgaaa gggccgaatt
      301 caagcttcgt gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
      361 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
      421 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
      481 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
      541 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
      601 gtgccttgaa ttacttccac ctggctccag tacgtgattc ttgatcccga gctggagcca
      661 ggggcgggcc ttgcgcttta ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg
      721 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
      781 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
      841 atagtcttgt aaatgcgggc caggatctgc acactggtat ttcggttttt ggggccgcgg
      901 gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
      961 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
     1021 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
     1081 cgtgagcgga aagatggccg cttcccggcc ctgctccagg gggctcaaaa tggaggacgc
     1141 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaggggcc tttccgtcct
     1201 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
     1261 tctggagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     1321 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     1381 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     1441 ttcaaagttt ttttcttcca tttcaggtgt cgtgaacacg ctaccgggcc accatggccg
     1501 aagggagcgt ggcaagacag cccgatctgc tgacatgcga cgacgaacca atccacattc
     1561 caggagctat ccagcctcat ggactgctgc tggctctggc cgctgacatg actatcgtgg
     1621 caggctctga taacctgcca gagctgaccg ggctggctat cggagcactg attggccggt
     1681 ccgcagccga cgtgttcgat tctgagactc acaatagact gaccatcgcc ctggctgaac
     1741 ctggggctgc agtgggagcc ccaattaccg tcggcttcac aatgcgaaag gacgctggct
     1801 ttatcgggtc ctggcaccgc catgatcagc tgatttttct ggagctggaa ccacctcaga
     1861 gagacgtggc agagccacag gctttctttc ggagaacaaa cagcgccatc aggcgcctgc
     1921 aggccgctga gactctggaa tccgcttgcg cagccgctgc acaggaagtg aggaagatca
     1981 ccgggttcga tcgcgtcatg atctacagat tcgcctcaga ctttagcgga gaagtgatcg
     2041 cagaagatcg ctgtgccgag gtcgaaagta aactgggcct gcattaccca gcctcaaccg
     2101 tgcctgcaca ggcacgacgg ctgtatacaa tcaaccccgt gcggatcatt cccgacatta
     2161 attacagacc agtgcccgtc acacctgacc tgaacccagt gactgggagg cccatcgatc
     2221 tgagcttcgc cattctgcgc agcgtgtccc ctgtccacct ggagtttatg aggaatatcg
     2281 gaatgcatgg caccatgtct atcagtattc tgagaggcga aaggctgtgg gggctgatcg
     2341 tgtgccacca tcggacacct tactatgtgg acctggacgg caggcaggct tgtgaactgg
     2401 tggctcaggt gctggcttgg cagattggcg tgatggaaga agagggcaga ggaagtcttc
     2461 taacatgcgg tgacgtggag gagaatcccg gccctatgtc tatgaccgag tacaagccca
     2521 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     2581 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     2641 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     2701 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     2761 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     2821 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     2881 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     2941 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     3001 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     3061 gaccgcgcac ctggtgcatg acccgcaagc ccggtgccgc cacgaacttc tctctgttaa
     3121 agcaagcagg agacgtggaa gaaaaccccg gtcctatgtc tagactggac aagagcaaag
     3181 tcataaacgg cgctctggaa ttactcaatg gagtcggtat cgaaggcctg acgacaagga
     3241 aactcgctca aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc
     3301 gggccctgct cgatgccctg ccaatcgaga tgctggacag gcatcatacc cacttctgcc
     3361 ccctggaagg cgagtcatgg caagactttc tgcggaacaa cgccaagtca ttccgctgtg
     3421 ctctcctctc acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac
     3481 agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga
     3541 acgcactgta cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac
     3601 aggagcatca agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac
     3661 ttctgagaca agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg
     3721 gcctggaact aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc ggcgggccgg
     3781 ccgacgccct tgacgatttt gacttagaca tgctcccagc cgatgccctt gacgactttg
     3841 accttgatat gctgcctgct gacgctcttg acgattttga ccttgacatg ctccccgggt
     3901 aactaagtaa ggatcctcta gagttaacat cgagggatca agcttatcga taatcaacct
     3961 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     4021 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     4081 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     4141 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     4201 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     4261 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     4321 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     4381 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     4441 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     4501 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgagaccta
     4561 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
     4621 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga
     4681 ccaatgactt acaaggcagc tgtagatctc tagataactg atcataatca gccataccac
     4741 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     4801 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     4861 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     4921 tttgtccaaa ctcatcaatg tatcttaacg ctcgagtgca tggatccttt gttactttat
     4981 agaagaaatt ttgagttttt gttttttttt aataaataaa taaacataaa taaattgttt
     5041 gttgaattta ttattagtat gtaagtgtaa atataataaa acttaatatc tattcaaatt
     5101 aataaataaa cctcgatata cagaccgata aaacacatgc gtcaatttta cgcatgatta
     5161 tctttaacgt acgtcacaat atgattatct ttctagggtt aaagagctcc agcttttgtt
     5221 ccctttagtg agggttaatt tcgagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     5281 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     5341 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     5401 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     5461 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     5521 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     5581 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     5641 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     5701 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     5761 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     5821 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     5881 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     5941 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     6001 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     6061 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     6121 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     6181 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     6241 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     6301 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     6361 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     6421 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     6481 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     6541 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     6601 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     6661 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     6721 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     6781 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     6841 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     6901 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     6961 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     7021 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     7081 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     7141 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     7201 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     7261 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     7321 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     7381 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa
     7441 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     7501 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     7561 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     7621 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     7681 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     7741 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     7801 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     7861 gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     7921 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     7981 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga
     8041 ctcactatag ggcgaattgg gtaccttaac cctagaaaga tagtctgcgt aaaattgacg
     8101 catgcattct tgaaatattg ctctctcttt ctaaatagcg cgaatccgtc gctgtgcatt
     8161 taggacatct cagtcgccgc ttggagctcc cgtgaggcgt gcttgtcaat gcggtaagtg
     8221 tcactgattt tgaactataa cgaccgcgtg agtcaaaatg acgcatgatt atcttttacg
     8281 tgacttttaa gatttaactc atacgataat tatattgtta tttcatgttc tacttacgtg
     8341 ataacttatt atatatatat tttcttgtta tagatatctc cccagcatgc ctgctattgt
     8401 cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc ccccagaata gaatgacacc
     8461 tactcagaca atgcgatgca atttcctcat tttattagga aaggacagtg ggagtggcac
     8521 cttccagggt caaggaaggc acgggggagg ggcaaacaac agatggctgg caactagaag
     8581 gcacagatcg tcgagaccgg tagcatcacc actttgtaca agaaagctgg gtcggcgcgc
     8641 ccacccttct agatacaatc cctggctatg gggaggtgag gtgcatagcg gtgcttgtcg
     8701 ggaggtgggg gctgccaata gtccatgtct gacccggccg ggctggcggg cgataaagtg
     8761 cagctgtagg gggaggtgga attggaggac acggaggagg acggcgcggg gctgttggtg
     8821 caactccacg tggaggcggg cgaggggctg tctccgctgc tgctcagggc ggcgctggcg
     8881 cctcccgggc tcagcaacac tgcctcggag aagagcgccc ccggcaggcc cccgccgccg
     8941 cccccgcagt gatccgccag gcgcagggtc tcggtgagtg cccagatgta gttgtgggcg
     9001 aagcgcaggg tctcgatctt ggtgagcttg gcgtcctcgg ggaacgtggg gagcacctcg
     9061 cgcagcgcgt ccagtgccgc gttgaggttg tgcatgcggt ttcgctcgcg gttgttggcc
     9121 ttcagtctac gggtcttctt gatgcgctgc accgtctcgg ccgtcttggc gcctcgggag
     9181 acggcccgcg cccgggaagg gcgccgtttg caatcgtgta ccagacccag cagccgtgcg
     9241 ggccggcagc cctccgcacc cgcagccacg ccgccccgcg ccccctgccc ggcctcagcc
     9301 ccgcgctgcc gacgcgcccc acctgacgcg cccggctcct cctcctcttc ttcgtcggcg
     9361 ctggatgaca gcggggtcag ggccgccaag gcgggggagg ccgatccgag cagcactaac
     9421 acgtcctctt cctccttcaa ctccaaggtc tcggatttga cgaacatggt gaagggggcg
     9481 gccgcggagc ctgctttttt gtacaaactt gtgatgctag cggcgatctg acggttcact
     9541 aaacgagctc tgcttatata
//
