LOCUS       sequence-306497-       10022 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast expression of Ost4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10022)
  AUTHORS   Jiang Z, Jin X, Li Y, Liu S, Liu XM, Wang YY, Zhao P, Cai X, Liu Y, 
            Tang Y, Sun X, Liu Y, Hu Y, Li M, Cai G, Qi X, Chen S, Du LL, He W
  TITLE     Genetically encoded tags for direct synthesis of EM-visible gold 
            nanoparticles in cells.
  JOURNAL   Nat Methods. 2020 Sep;17(9):937-946. doi: 10.1038/s41592-020-0911-z.
            Epub 2020 Aug 10.
  PUBMED    32778831
REFERENCE   2  (bases 1 to 10022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-0911-z"; journalName: "Nat Methods"; date: 
            "2020-09"; volume: "17"; issue: "9"; pages: "937-946"
COMMENT     Sequence Label: pDUAL-Pnmt1-Ost4-GFP-MTalpha
FEATURES             Location/Qualifiers
     source          1..10022
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    288..309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(324..329,330..347,348..354)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    362..378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     367..389
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     386..402
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     386..402
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1733..2446
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      2572..2759
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2627..2651)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(4423..5217)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     rep_origin      5826..6609
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(7332..7349)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7332..7348)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7341..7363)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7561..8016
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7648..7667)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7858..7879
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8033..8052)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8152..8174
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8212..8230)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8298..8402
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8403..8471,8472..9263)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8621..8640)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9434..10022
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9923..9942
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      241 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      301 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      361 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt
      421 gcatgcctgc aggtcgatcg actctagagg atcagaaaat tatcgccata aaagacagaa
      481 taagtcatca gcggttgttt catttcctat attttttttt tattttttta ttttttaata
      541 agggaaaatt taacgtctaa ggatacagaa gattgttagc acattaaagt aataaaggct
      601 taagtagtaa gtgccttagc atgttattgt atttcaaagg acataatcta aaataataac
      661 aatatcattt ctcacaagtt attcaatttt cttttttttt tctaataata tcaagaatgt
      721 attatttgtt tgacataagt caactaattt atttaatatg ctggattaat cttgcagaca
      781 tgtaaattaa caagttttag tcaaataacg ttgaagtttc aatgaactca aataatttct
      841 cttttttttt atataaccat aatctgattt atattttccg cagggatcaa ctgaagttat
      901 gacatttgga ttggatcact tataaccttg gtcgccaaat aatacaaaaa tcagcgttat
      961 aaaacaaaga aggtttttgt taagaaatta atcctctttc ttgataagaa agttgaaccg
     1021 aaattgcaga tactgatata tgaaaataat acccacaatt ttgggaatag cgcaagcctc
     1081 aatttaaaca ataggtgagg acacatgata atgacctcaa tgattgttag aagaaaagag
     1141 cctcattaca aaatcgaaaa atgaatggtt gggtacaagt ttccaaaaca tggtaaagtg
     1201 gactttgcgt atgagacgta aatagaaaaa aacacttgtt atatgttttc tagaattatt
     1261 gttgtctctt tatggttgga tgatgcaaaa tagtaatttc ggttagttgc tgtaaaacac
     1321 cacgagacaa atagatatgg atatttatta aatcaggaaa aacgtaactc tcggctactg
     1381 gatggttcag tcacccaacg attactgggg agagaaaaca gggcaaaagc aaagcttaaa
     1441 ggaatccgat tgtcattcgg caatgtgcag cgaaactaaa aaccggataa tggacctgtt
     1501 aatcgaaaca ttgaagatat ataaaggaag aggaatcctg gcatatcatc aattgaataa
     1561 gttgaattaa ttatttcaat ctcattctca ctttctgact tatagtcgct ttgttaaagc
     1621 tagcggatcc atgacagacg ttcaacttca aaatatcgtt accacctttg gtatctccat
     1681 gatgctctta atcatccttt accattacct ttcacgtcct caagcaggat ccatgtctaa
     1741 aggtgaagaa ttattcactg gtgttgtccc aattttggtt gaattagatg gtgatgttaa
     1801 tggtcacaaa ttttctgtct ccggtgaagg tgaaggtgat gctacttacg gtaaattgac
     1861 cttaaaattt atttgtacta ctggtaaatt gccagttcca tggccaacct tagtcactac
     1921 tttcggttat ggtgttcaat gttttgcgag atacccagat catatgaaac aacatgactt
     1981 tttcaagtct gccatgccag aaggttatgt tcaagaaaga actatttttt tcaaagatga
     2041 cggtaactac aagaccagag ctgaagtcaa gtttgaaggt gataccttag ttaatagaat
     2101 cgaattaaaa ggtattgatt ttaaagaaga tggtaacatt ttaggtcaca aattggaata
     2161 caactataac tctcacaatg tttacatcat ggctgacaaa caaaagaatg gtatcaaagt
     2221 taacttcaaa attagacaca acattgaaga tggttctgtt caattagctg accattatca
     2281 acaaaatact ccaattggtg atggtccagt cttgttacca gacaaccatt acttatccac
     2341 tcaatctgcc ttatccaaag atccaaacga aaagagagac cacatggtct tgttagaatt
     2401 tgttactgct gctggtatta cccatggtat ggatgaattg tacaaaagat ctatggcatg
     2461 ctgcgcctgc tgtcccgtgg gctgcgccgc atgtgccgca ggctgtgtct gcgcaggcgc
     2521 cgcggacgcg tgcgcgtgct gtgccagatc tggcgcgcca cttctaaata agcgaatttc
     2581 ttatgattta tgatttttat tattaaataa gttataaaaa aaataagtgt atacaaattt
     2641 taaagtgact cttaggtttt aaaacgaaaa ttcttattct tgagtaactc tttcctgtag
     2701 gtcaggttgc tttctcaggt atagtatgag gtcgctctta ttgaccacac ctctaccggc
     2761 agatccgcta gggataacag ggtaatatac tagtaagatg attcttttat cgtcgataac
     2821 gatgaggatg tagacgatta tgatgagagt gattagcttc taaaatttgg tgttttcaaa
     2881 cgctatttac agtacaatct gaaaaaattg gatgaacatt gtaaatggta ggatacgagt
     2941 tccaattaat gtatttttta agtattacac ttagcttatt aattttatat atacattcta
     3001 cctctgtggg tttaaagaca actcgtacac atactcaaat tatttatgaa cattataatg
     3061 catattcgta ctgtctatta aacatttttt tgacattaaa aatttcgttt actaacgtag
     3121 aaagcgctga taattgaggc tactatttta atatctagaa acaaagattc attaattaat
     3181 taagtaagct ccaggatact tgtatatttc gttaaaaaag cgcaatttca acaattccta
     3241 tgaacatcca tgatttcatc acaaaatcaa acatatcagg atttaacgtg aattatttgc
     3301 tttcttgtga ttttcatctg atttctggtc atttacgtta ctgtaaacaa gctttttaaa
     3361 aaaaggattt tttacgcaat attatcgata tcccaatctg tagtataaaa agtgcaacag
     3421 tgttgagttt cccgaaacca ggcatttttg cggtagggtc tgaaactttc acgaaaccat
     3481 ttcacaacaa aacttcttca acaacatgtg tgcaaagaaa atcgtcgtct taccaggaga
     3541 ccatattggc cctgaaattg ttgcttctgc cttggaggtt ttgaaagtcg ttgagaagaa
     3601 gcgacctgag ttaaaactcg agtttgaaga acacaagatt ggaggtgcct ctattgatgc
     3661 ctatggaacc cctttgactg acgagactgt gaaggcttgt ttggaagctg acggtgttct
     3721 tttgggtgcc gttggtggtc ctgaatggac caaccccaat tgtcgtcctg agcaaggttt
     3781 attgaagctt cgtaagagta tgggtgtttg ggccaacctt cgaccttgca actttgccag
     3841 caagtcttta gtcaagtaca gccctttgaa gcctgaaatc gttgaaggtg tcgatttttg
     3901 tgttgtacga gaacttactg gaggttgtta ctttggtgag cgcactgagg acaacggatc
     3961 gggttatgct atggacactt ggccttacag tttggaagaa gtttctcgta ttgctcgttt
     4021 ggcggccgcg ggcccaacac caatgtttat aaccaagttt tatcttgttt gtctacatgg
     4081 tattttacat tcatctacat acatctttca ttggctttgt acatagttat cattacaagt
     4141 ctaaaaaaat tcactctttt cttattcaat gtcaatccaa gagaaaagat tgtggtaatg
     4201 ttgtaggagc atgtttaata aattactata gcaaattact ttttattccc aaggtgttta
     4261 tctataatag ttaatatttt agtcgctaca taaaatttta ccaaagagta cttgtatact
     4321 aattctaaat gccttctgac ataaaacgcc taggaaaaca aacgcaaaca aggcatcgac
     4381 tttttcaata accaaccaaa aaaattttac attagtcttt ttttaatgct gagaaagtct
     4441 ttgctgatat gccttccaac cagcttctct atatctcttg gcttcgacaa caggattacg
     4501 accagctcca tagactccac gaccaacaat gatgatgtcg ctaccgcagt ttacaatcac
     4561 ttcttcagga gtacgatatt gctgtcccag cccgtctcct ttaacatcca agccgatacc
     4621 aggggacata gttatgtagt cgctttgaag gttaggaaat cgacgaccag ctataaagcc
     4681 aaagcaaaaa tcggtatgct tctcaaacca ttctaaggtt ttctctgtgt aggaaccagt
     4741 agccaaagag cctttggaag acatttcagc caaaagcaag agaccacgtc ccaaaggtaa
     4801 accaacttct ttgaggcctt gtataatacc ctcgcctggc actgtatggc aatttgtgat
     4861 atgagcccaa gaagcaattt tgtacacacc agatgcatat tgtagcttga cggtatttcc
     4921 aatgtctgcg aatttgcgat cctcaaagat aagaaaacga tgctttttac ctaaggccac
     4981 cagtttttct accatatcct ggtcgaaatc ctcgacaacg tcaatatgtg tcttgataac
     5041 acagacatag ggtccaattt tatctaccaa ttctaagatt tcggatttct tcgtcaaatc
     5101 gaccgcgact gacaagttgc tttgcttttc ttccatcaaa gccaacaatt ccttggcaat
     5161 gggatttttc atcccctcag ctctagctga atagctttga aatactctag catccataac
     5221 tttgctttta aacctttaat ttcgatccaa gcaaaaaaga ggttcttggt aggacaatac
     5281 ggtaagaaaa cacgacatgt gcagagatgc cgacgaagca tagttaaact gggatggtaa
     5341 aatcaattaa gaatttataa agacaaaatt gtataagtct ctaaaacatc ttaattatac
     5401 ctcacagaac tatctaaaat atattcacaa agtgcaaaca ttatcatgaa aaagaaccat
     5461 tttaatttaa agcaagggca ttaaggctta tttacagaat ttcttacttt tgtaaagatt
     5521 ataaggctga ttatcttttt caccatgcca aaaattacac aagatagaat ggatgtttga
     5581 aattaaacgt gagtatacaa acaaatacac taggtaaatc gaaacatttt tttctccatt
     5641 aagtaacaaa ttcctattta gagaaagaat gctgagtaga ttaaataatc tatacaaact
     5701 tttttaacac aaatgcatac atatagccag tgggatttgt agctaagctt gcgaattcga
     5761 gtctaactcc ttaaccactc ggacatagtg acttatctga cactcattgt aaattaatat
     5821 atatataggc attttgttta gttaaaggta cttaagtaat tagtataaac gaaccaattt
     5881 tataatcagg aagttaagtg aatggtagca catgtcgtaa aaattgtgaa tttttattga
     5941 ataatatttt aaatacaagc ctttctagac taggtatact cataaacata tatgagcaaa
     6001 aggatagagg agattacatt gcatcttcta caaatttatt tattgccctt tactgaaaaa
     6061 ttaaataatg agtactaaat gataaaaagc gctcagtaca agaaagattg caaaaatatt
     6121 gcattcttca tgaattaaag ttgcatataa ggcatattga aagtaatagt actaaaacag
     6181 cagttagcga aaattaatag aattatattc gcaagacaat tgtgacatta aattaaaaaa
     6241 ttgtagaatt tttactatcc tctttaacgc catgagcctt tataaaaagg ttaaattagt
     6301 tttaacattc tttttttgag taagagttta caatttatca aaacctgtgt tattatattc
     6361 attagtttca atttattagc atctagagaa aaatcaattg gcagttacat tgttggaatt
     6421 tatgaagaaa aagaatctac aacggagaat aagttgctga tcgctttccc taaaattgta
     6481 tattttgctg agcttatttt gacatttcgt tgaagttttc tctaattcgc attcatttta
     6541 agtaaaacaa tgagaaataa aattacaaaa aatacaaatt aaaatacaat ttttagctat
     6601 aattatagac gatgcccttg tatcccattc tgtctcgctt gcccctactt tttatctttt
     6661 atataccata atgaacgctg ccgctactaa ccataccccg attttacatt tcggactccc
     6721 aaggacgtac aaaatagaaa actatagaaa aaaataatca gaaaatagca tgtcatctct
     6781 ttgtaaaacg cgtttgcaag aagaaaggaa acaatggaga agagatcatc catttgtatg
     6841 taaattttag taaacttgaa gaaatcacta acaacttctc ttacttaggg attctatgca
     6901 aaaccttgta aatcatctga tggaggactc gatttaatga attggaaggt tgggcccgcg
     6961 gccgcttagc tgaaacttcc aaccctcctg ctcccgtcac attactcgac aaagctaatg
     7021 ttttggcaac ttctcgtctt tggcgcaaga ctgtagctaa gatttttaag gaggaatatc
     7081 ctcaccttac cttaaaaaac cagctcattg actccgcagc catgcttttg gtcaagagcc
     7141 ctcgtacact taacggtgtt gttttgactg acaacttgtt tggtgacatt atttcagatg
     7201 aggcttctgt cattcctggt agcttgggcc ttttgccttc tgcctccctt tccggtgtgg
     7261 taggaaaatc agaagaaaag gttcattgtt tggttgagcc cattcacggt agcgctcccg
     7321 agctcgaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     7381 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     7441 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
     7501 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt
     7561 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     7621 ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     7681 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     7741 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
     7801 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     7861 gactcttgtt ccaaactgga acaacactca actctatctc gggctattct tttgatttat
     7921 aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta
     7981 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct
     8041 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     8101 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     8161 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     8221 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     8281 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     8341 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     8401 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     8461 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     8521 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     8581 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     8641 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     8701 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     8761 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     8821 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     8881 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     8941 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     9001 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     9061 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     9121 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     9181 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     9241 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     9301 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     9361 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     9421 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     9481 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     9541 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     9601 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     9661 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     9721 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     9781 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     9841 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     9901 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     9961 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    10021 aa
//
