LOCUS       sequence-306503-       10112 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast expression of Ost4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10112)
  AUTHORS   Jiang Z, Jin X, Li Y, Liu S, Liu XM, Wang YY, Zhao P, Cai X, Liu Y, 
            Tang Y, Sun X, Liu Y, Hu Y, Li M, Cai G, Qi X, Chen S, Du LL, He W
  TITLE     Genetically encoded tags for direct synthesis of EM-visible gold 
            nanoparticles in cells.
  JOURNAL   Nat Methods. 2020 Sep;17(9):937-946. doi: 10.1038/s41592-020-0911-z.
            Epub 2020 Aug 10.
  PUBMED    32778831
REFERENCE   2  (bases 1 to 10112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-0911-z"; journalName: "Nat Methods"; date: 
            "2020-09"; volume: "17"; issue: "9"; pages: "937-946"
COMMENT     Sequence Label: pDUAL-Pnmt1-Ost4-GFP-MTn
FEATURES             Location/Qualifiers
     source          1..10112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    288..309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(324..329,330..347,348..354)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    362..378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     367..389
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     386..402
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     386..402
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1733..2446
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      2662..2849
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2717..2741)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(4513..5307)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     rep_origin      5916..6699
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(7422..7439)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7422..7438)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7431..7453)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7651..8106
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7738..7757)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7948..7969
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8123..8142)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8242..8264
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8302..8320)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8388..8492
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8493..8561,8562..9353)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8711..8730)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9524..10112
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10013..10032
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      241 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      301 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      361 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt
      421 gcatgcctgc aggtcgatcg actctagagg atcagaaaat tatcgccata aaagacagaa
      481 taagtcatca gcggttgttt catttcctat attttttttt tattttttta ttttttaata
      541 agggaaaatt taacgtctaa ggatacagaa gattgttagc acattaaagt aataaaggct
      601 taagtagtaa gtgccttagc atgttattgt atttcaaagg acataatcta aaataataac
      661 aatatcattt ctcacaagtt attcaatttt cttttttttt tctaataata tcaagaatgt
      721 attatttgtt tgacataagt caactaattt atttaatatg ctggattaat cttgcagaca
      781 tgtaaattaa caagttttag tcaaataacg ttgaagtttc aatgaactca aataatttct
      841 cttttttttt atataaccat aatctgattt atattttccg cagggatcaa ctgaagttat
      901 gacatttgga ttggatcact tataaccttg gtcgccaaat aatacaaaaa tcagcgttat
      961 aaaacaaaga aggtttttgt taagaaatta atcctctttc ttgataagaa agttgaaccg
     1021 aaattgcaga tactgatata tgaaaataat acccacaatt ttgggaatag cgcaagcctc
     1081 aatttaaaca ataggtgagg acacatgata atgacctcaa tgattgttag aagaaaagag
     1141 cctcattaca aaatcgaaaa atgaatggtt gggtacaagt ttccaaaaca tggtaaagtg
     1201 gactttgcgt atgagacgta aatagaaaaa aacacttgtt atatgttttc tagaattatt
     1261 gttgtctctt tatggttgga tgatgcaaaa tagtaatttc ggttagttgc tgtaaaacac
     1321 cacgagacaa atagatatgg atatttatta aatcaggaaa aacgtaactc tcggctactg
     1381 gatggttcag tcacccaacg attactgggg agagaaaaca gggcaaaagc aaagcttaaa
     1441 ggaatccgat tgtcattcgg caatgtgcag cgaaactaaa aaccggataa tggacctgtt
     1501 aatcgaaaca ttgaagatat ataaaggaag aggaatcctg gcatatcatc aattgaataa
     1561 gttgaattaa ttatttcaat ctcattctca ctttctgact tatagtcgct ttgttaaagc
     1621 tagcggatcc atgacagacg ttcaacttca aaatatcgtt accacctttg gtatctccat
     1681 gatgctctta atcatccttt accattacct ttcacgtcct caagcaggat ccatgtctaa
     1741 aggtgaagaa ttattcactg gtgttgtccc aattttggtt gaattagatg gtgatgttaa
     1801 tggtcacaaa ttttctgtct ccggtgaagg tgaaggtgat gctacttacg gtaaattgac
     1861 cttaaaattt atttgtacta ctggtaaatt gccagttcca tggccaacct tagtcactac
     1921 tttcggttat ggtgttcaat gttttgcgag atacccagat catatgaaac aacatgactt
     1981 tttcaagtct gccatgccag aaggttatgt tcaagaaaga actatttttt tcaaagatga
     2041 cggtaactac aagaccagag ctgaagtcaa gtttgaaggt gataccttag ttaatagaat
     2101 cgaattaaaa ggtattgatt ttaaagaaga tggtaacatt ttaggtcaca aattggaata
     2161 caactataac tctcacaatg tttacatcat ggctgacaaa caaaagaatg gtatcaaagt
     2221 taacttcaaa attagacaca acattgaaga tggttctgtt caattagctg accattatca
     2281 acaaaatact ccaattggtg atggtccagt cttgttacca gacaaccatt acttatccac
     2341 tcaatctgcc ttatccaaag atccaaacga aaagagagac cacatggtct tgttagaatt
     2401 tgttactgct gctggtatta cccatggtat ggatgaattg tacaaaagat ctatggaccc
     2461 cgcctgtgcg tgtgcagccg gcggcgcttg cgcttgcgca ggcgcttgcg cctgcgcagc
     2521 atgcgcatgt gccgcttgcg cagcaagctg ctgcgcctgc tgtcccgtgg gctgcgccgc
     2581 atgtgccgca ggctgtgtct gcgcaggcgc cgcggacgcg tgcgcgtgct gtgccagatc
     2641 tggcgcgcca cttctaaata agcgaatttc ttatgattta tgatttttat tattaaataa
     2701 gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt aaaacgaaaa
     2761 ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt atagtatgag
     2821 gtcgctctta ttgaccacac ctctaccggc agatccgcta gggataacag ggtaatatac
     2881 tagtaagatg attcttttat cgtcgataac gatgaggatg tagacgatta tgatgagagt
     2941 gattagcttc taaaatttgg tgttttcaaa cgctatttac agtacaatct gaaaaaattg
     3001 gatgaacatt gtaaatggta ggatacgagt tccaattaat gtatttttta agtattacac
     3061 ttagcttatt aattttatat atacattcta cctctgtggg tttaaagaca actcgtacac
     3121 atactcaaat tatttatgaa cattataatg catattcgta ctgtctatta aacatttttt
     3181 tgacattaaa aatttcgttt actaacgtag aaagcgctga taattgaggc tactatttta
     3241 atatctagaa acaaagattc attaattaat taagtaagct ccaggatact tgtatatttc
     3301 gttaaaaaag cgcaatttca acaattccta tgaacatcca tgatttcatc acaaaatcaa
     3361 acatatcagg atttaacgtg aattatttgc tttcttgtga ttttcatctg atttctggtc
     3421 atttacgtta ctgtaaacaa gctttttaaa aaaaggattt tttacgcaat attatcgata
     3481 tcccaatctg tagtataaaa agtgcaacag tgttgagttt cccgaaacca ggcatttttg
     3541 cggtagggtc tgaaactttc acgaaaccat ttcacaacaa aacttcttca acaacatgtg
     3601 tgcaaagaaa atcgtcgtct taccaggaga ccatattggc cctgaaattg ttgcttctgc
     3661 cttggaggtt ttgaaagtcg ttgagaagaa gcgacctgag ttaaaactcg agtttgaaga
     3721 acacaagatt ggaggtgcct ctattgatgc ctatggaacc cctttgactg acgagactgt
     3781 gaaggcttgt ttggaagctg acggtgttct tttgggtgcc gttggtggtc ctgaatggac
     3841 caaccccaat tgtcgtcctg agcaaggttt attgaagctt cgtaagagta tgggtgtttg
     3901 ggccaacctt cgaccttgca actttgccag caagtcttta gtcaagtaca gccctttgaa
     3961 gcctgaaatc gttgaaggtg tcgatttttg tgttgtacga gaacttactg gaggttgtta
     4021 ctttggtgag cgcactgagg acaacggatc gggttatgct atggacactt ggccttacag
     4081 tttggaagaa gtttctcgta ttgctcgttt ggcggccgcg ggcccaacac caatgtttat
     4141 aaccaagttt tatcttgttt gtctacatgg tattttacat tcatctacat acatctttca
     4201 ttggctttgt acatagttat cattacaagt ctaaaaaaat tcactctttt cttattcaat
     4261 gtcaatccaa gagaaaagat tgtggtaatg ttgtaggagc atgtttaata aattactata
     4321 gcaaattact ttttattccc aaggtgttta tctataatag ttaatatttt agtcgctaca
     4381 taaaatttta ccaaagagta cttgtatact aattctaaat gccttctgac ataaaacgcc
     4441 taggaaaaca aacgcaaaca aggcatcgac tttttcaata accaaccaaa aaaattttac
     4501 attagtcttt ttttaatgct gagaaagtct ttgctgatat gccttccaac cagcttctct
     4561 atatctcttg gcttcgacaa caggattacg accagctcca tagactccac gaccaacaat
     4621 gatgatgtcg ctaccgcagt ttacaatcac ttcttcagga gtacgatatt gctgtcccag
     4681 cccgtctcct ttaacatcca agccgatacc aggggacata gttatgtagt cgctttgaag
     4741 gttaggaaat cgacgaccag ctataaagcc aaagcaaaaa tcggtatgct tctcaaacca
     4801 ttctaaggtt ttctctgtgt aggaaccagt agccaaagag cctttggaag acatttcagc
     4861 caaaagcaag agaccacgtc ccaaaggtaa accaacttct ttgaggcctt gtataatacc
     4921 ctcgcctggc actgtatggc aatttgtgat atgagcccaa gaagcaattt tgtacacacc
     4981 agatgcatat tgtagcttga cggtatttcc aatgtctgcg aatttgcgat cctcaaagat
     5041 aagaaaacga tgctttttac ctaaggccac cagtttttct accatatcct ggtcgaaatc
     5101 ctcgacaacg tcaatatgtg tcttgataac acagacatag ggtccaattt tatctaccaa
     5161 ttctaagatt tcggatttct tcgtcaaatc gaccgcgact gacaagttgc tttgcttttc
     5221 ttccatcaaa gccaacaatt ccttggcaat gggatttttc atcccctcag ctctagctga
     5281 atagctttga aatactctag catccataac tttgctttta aacctttaat ttcgatccaa
     5341 gcaaaaaaga ggttcttggt aggacaatac ggtaagaaaa cacgacatgt gcagagatgc
     5401 cgacgaagca tagttaaact gggatggtaa aatcaattaa gaatttataa agacaaaatt
     5461 gtataagtct ctaaaacatc ttaattatac ctcacagaac tatctaaaat atattcacaa
     5521 agtgcaaaca ttatcatgaa aaagaaccat tttaatttaa agcaagggca ttaaggctta
     5581 tttacagaat ttcttacttt tgtaaagatt ataaggctga ttatcttttt caccatgcca
     5641 aaaattacac aagatagaat ggatgtttga aattaaacgt gagtatacaa acaaatacac
     5701 taggtaaatc gaaacatttt tttctccatt aagtaacaaa ttcctattta gagaaagaat
     5761 gctgagtaga ttaaataatc tatacaaact tttttaacac aaatgcatac atatagccag
     5821 tgggatttgt agctaagctt gcgaattcga gtctaactcc ttaaccactc ggacatagtg
     5881 acttatctga cactcattgt aaattaatat atatataggc attttgttta gttaaaggta
     5941 cttaagtaat tagtataaac gaaccaattt tataatcagg aagttaagtg aatggtagca
     6001 catgtcgtaa aaattgtgaa tttttattga ataatatttt aaatacaagc ctttctagac
     6061 taggtatact cataaacata tatgagcaaa aggatagagg agattacatt gcatcttcta
     6121 caaatttatt tattgccctt tactgaaaaa ttaaataatg agtactaaat gataaaaagc
     6181 gctcagtaca agaaagattg caaaaatatt gcattcttca tgaattaaag ttgcatataa
     6241 ggcatattga aagtaatagt actaaaacag cagttagcga aaattaatag aattatattc
     6301 gcaagacaat tgtgacatta aattaaaaaa ttgtagaatt tttactatcc tctttaacgc
     6361 catgagcctt tataaaaagg ttaaattagt tttaacattc tttttttgag taagagttta
     6421 caatttatca aaacctgtgt tattatattc attagtttca atttattagc atctagagaa
     6481 aaatcaattg gcagttacat tgttggaatt tatgaagaaa aagaatctac aacggagaat
     6541 aagttgctga tcgctttccc taaaattgta tattttgctg agcttatttt gacatttcgt
     6601 tgaagttttc tctaattcgc attcatttta agtaaaacaa tgagaaataa aattacaaaa
     6661 aatacaaatt aaaatacaat ttttagctat aattatagac gatgcccttg tatcccattc
     6721 tgtctcgctt gcccctactt tttatctttt atataccata atgaacgctg ccgctactaa
     6781 ccataccccg attttacatt tcggactccc aaggacgtac aaaatagaaa actatagaaa
     6841 aaaataatca gaaaatagca tgtcatctct ttgtaaaacg cgtttgcaag aagaaaggaa
     6901 acaatggaga agagatcatc catttgtatg taaattttag taaacttgaa gaaatcacta
     6961 acaacttctc ttacttaggg attctatgca aaaccttgta aatcatctga tggaggactc
     7021 gatttaatga attggaaggt tgggcccgcg gccgcttagc tgaaacttcc aaccctcctg
     7081 ctcccgtcac attactcgac aaagctaatg ttttggcaac ttctcgtctt tggcgcaaga
     7141 ctgtagctaa gatttttaag gaggaatatc ctcaccttac cttaaaaaac cagctcattg
     7201 actccgcagc catgcttttg gtcaagagcc ctcgtacact taacggtgtt gttttgactg
     7261 acaacttgtt tggtgacatt atttcagatg aggcttctgt cattcctggt agcttgggcc
     7321 ttttgccttc tgcctccctt tccggtgtgg taggaaaatc agaagaaaag gttcattgtt
     7381 tggttgagcc cattcacggt agcgctcccg agctcgaatt cactggccgt cgttttacaa
     7441 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     7501 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     7561 agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     7621 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
     7681 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc
     7741 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     7801 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     7861 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     7921 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     7981 actctatctc gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt
     8041 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     8101 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     8161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     8221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     8281 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     8341 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     8401 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     8461 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     8521 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     8581 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     8641 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     8701 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     8761 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     8821 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     8881 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     8941 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     9001 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     9061 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     9121 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     9181 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     9241 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     9301 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     9361 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     9421 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     9481 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     9541 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     9601 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     9661 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     9721 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     9781 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     9841 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     9901 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     9961 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    10021 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    10081 gatgctcgtc aggggggcgg agcctatgga aa
//
