LOCUS       sequence-306505-       13343 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast expression of Nup124.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13343)
  AUTHORS   Jiang Z, Jin X, Li Y, Liu S, Liu XM, Wang YY, Zhao P, Cai X, Liu Y, 
            Tang Y, Sun X, Liu Y, Hu Y, Li M, Cai G, Qi X, Chen S, Du LL, He W
  TITLE     Genetically encoded tags for direct synthesis of EM-visible gold 
            nanoparticles in cells.
  JOURNAL   Nat Methods. 2020 Sep;17(9):937-946. doi: 10.1038/s41592-020-0911-z.
            Epub 2020 Aug 10.
  PUBMED    32778831
REFERENCE   2  (bases 1 to 13343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-0911-z"; journalName: "Nat Methods"; date: 
            "2020-09"; volume: "17"; issue: "9"; pages: "937-946"
COMMENT     Sequence Label: pDUAL-P41nmt1-Nup124-GFP
FEATURES             Location/Qualifiers
     source          1..13343
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    127..148
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(163..168,169..186,187..193)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    201..217
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     206..228
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     225..241
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     225..241
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(1987..1998)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             4992..5705
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      5732..5919
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5787..5811)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(7583..8377)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     rep_origin      8986..9769
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(10492..10509)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10492..10508)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10501..10523)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10721..11176
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10808..10827)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11018..11039
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11193..11212)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11312..11334
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11372..11390)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11458..11562
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11563..11631,11632..12423)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11781..11800)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12594..13182
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13083..13102
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(13336..13343,1..10)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
       61 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
      121 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
      181 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
      241 ccatgattac gccaagcttg catgcctgca ggtcgatcga ctctagagga tcagaaaatt
      301 atcgccataa aagacagaat aagtcatcag cggttgtttc atttcctata tttttttttt
      361 atttttttat tttttaataa gggaaaattt aacgtctaag gatacagaag attgttagca
      421 cattaaagta ataaaggctt aagtagtaag tgccttagca tgttattgta tttcaaagga
      481 cataatctaa aataataaca atatcatttc tcacaagtta ttcaattttc tttttttttt
      541 ctaataatat caagaatgta ttatttgttt gacataagtc aactaattta tttaatatgc
      601 tggattaatc ttgcagacat gtaaattaac aagttttagt caaataacgt tgaagtttca
      661 atgaactcaa ataatttctc ttttttttta tataaccata atctgattta tattttccgc
      721 agggatcaac tgaagttatg acatttggat tggatcactt ataaccttgg tcgccaaata
      781 atacaaaaat cagcgttata aaacaaagaa ggtttttgtt aagaaattaa tcctctttct
      841 tgataagaaa gttgaaccga aattgcagat actgatatat gaaaataata cccacaattt
      901 tgggaatagc gcaagcctca atttaaacaa taggtgagga cacatgataa tgacctcaat
      961 gattgttaga agaaaagagc ctcattacaa aatcgaaaaa tgaatggttg ggtacaagtt
     1021 tccaaaacat ggtaaagtgg actttgcgta tgagacgtaa atagaaaaaa acacttgtta
     1081 tatgttttct agaattattg ttgtctcttt atggttggat gatgcaaaat agtaatttcg
     1141 gttagttgct gtaaaacacc acgagacaaa tagatatgga tatttattaa atcaggaaaa
     1201 acgtaactct cggctactgg atggttcagt cacccaacga ttactgggga gagaaaacag
     1261 ggcaaaagca aagcttaaag gaatccgatt gtcattcggc aatgtgcagc gaaactaaaa
     1321 accggataat ggacctgtta atcgaaacat tgaagataaa ggaagaggaa tcctggcata
     1381 tcatcaattg aataagttga attaattatt tcaatctcat tctcactttc tgacttatag
     1441 tcgctttgtt aaagctagcg gatccatgcc tcctgtttca aaaaatactc gcacttcatc
     1501 aaagactgtt aagaaaccct atgacccacc tcaggggagc tcaaggccct tttttacggt
     1561 cttgaaacgg gcattttcat ctgttttgca cccattcacc agtggtctag acgagaaagc
     1621 ttcaggaacg gctagcaagg atagaaagag tggaagagca ggtacaaaaa gcctgcttac
     1681 tccagagtta acacctcatt atttaggaaa atctccgtaa gttgacattt ttgtcgtgaa
     1741 acatattaac attaaataga agaattattc gcgttagtaa tcggagccat gttagaacaa
     1801 ttgatggaat agaggaaaaa gttcatacga atacatttga acctaggaag ccaaagcaaa
     1861 agcaagatta tactaattcc ccaacgcttt ttaaacgcca tgacgagttg tcgttaaaat
     1921 cacttaattc gcttcatcct tcttctgctt tatccaaaaa gttgggcagc acttctcaac
     1981 atcaaattgc aactcccaaa tcctctgctt cattgctcaa tatccttcgg tcacttcacg
     2041 atgagcagaa aaatacctta aatatatcaa gtgtaaaaca agatcgtatt accgaagcta
     2101 atcctacttg tgagaagcgc aagccttcta gaagtcccag tcctatgctt tccaaaaaga
     2161 aatctgttgc tcgtgcctct gaaaacgagc cctcagctaa gcagaacaaa agcttttctg
     2221 gaaatgattc ccataagagt cttaccgaca ttagagataa ggagaatggc gaaacagagg
     2281 tctcagcaaa aaatcacgtt cctcaccgct cctctcggcg acgtcgtaga caccagcggc
     2341 ttatccctat catttatgaa actcttgaac agatggattt aaggaagcct gtattagtca
     2401 atgcagaagt tcagacagat tccaaccccg gtaatacaat gtttattgat aagcaagata
     2461 tttatcaccg gttaagtaca cccacttcgc gtaagcgtca gactctcgag aaaggtcata
     2521 ttaaggcttt ttctgcagtt gatgaggatt tggatgaaat ctttgcttgc gaagatgacg
     2581 ttcattacac cgcattaccc aaacaaaatc caaaatctga gcgtatatta gaacctataa
     2641 tagcatctcc caaggataat acaagtgaca agggtttatt gacgaagtcc gcccctacct
     2701 ttgaagagct tcaagcctct attaccccta agcctgtaaa gacttctccg aatgatacgg
     2761 cacttactct cgcgaacgct gaggataata aaacctttga acatcagcca ttgtcaaagg
     2821 atacagaagc gcccaaaagc caattttcct catcgccgac aaaagaatct acaactagaa
     2881 agtccgaagt tgaaccgcca tcaccttcta aggaaatcaa atcttctcac ttttcagtac
     2941 ccgaatttaa gtttgaaccg aagactgaag ctaccacgga caaaaaatta aatgtaccca
     3001 aatttgagtt taagccaact gctactgctg atgtgcaaac taatcgtttg aaggagaatg
     3061 agcccaagcc aacgttcttt gcacaattac cgtcaaagac tcaagagacg ccaagtataa
     3121 ctgagaacaa gccttcattt ttttctcaat taagtccaaa acgagaagaa acagaaaaga
     3181 aggacaacgc tccgtctgct cctgcttcaa cttctggttt cagctttgga ggattcgcac
     3241 ctaaaaccct tgaagagaag gaagaaacga aagctcctac gttcaatttc tctttaaaca
     3301 atgcttctag tacacaagac acaacaaaac ctacgctaca atttaatttt ggaagtagtt
     3361 ttggaaagcc aacaagcaat attttcaatg acaaaaaaac ttctgaaaat ggtctagcta
     3421 gttctactgt tgcttctgag tcaaaaccgt cagctcccga atcaaaaccg tcctcgggtt
     3481 ttggaaatac cgcaggctca tcaccctttt cattcaattt gaccaaggag tccaaagaag
     3541 tcccacctac aaattccttt tcttttgcta aaaaagggaa agatgaagcg aatgacagtt
     3601 tgtcagctaa agcttctact ccattttcgt ttgcaaaacc taatactgaa aatgtaacaa
     3661 caactgctcc tcagttttca tttaatttta ccaagccaaa tacggatgcc aaaaccaatt
     3721 tgctacctga aaaaactttt aatgaagaag ccgtaaagca aaaggaaact gaaaaagagg
     3781 tccctccaac aggtccaaaa gcttctgaaa taaaggattc agtatcttct aataacgctg
     3841 tgccctcttc aacctttaat tttgtatctc cctttgctgc tgtatctgaa aaaacaaatg
     3901 agaataacat tcctaacgat acaacgaaga cgaatggcaa tgctactaag agaactttgg
     3961 aacaaacgga ggatgcaaag ccttttgctt tctccttcgg ttccactaca gaacaggcaa
     4021 acaaaaaagc ctcaacgtca aatgaaacta cgaagcctca attggataca tcttctaaaa
     4081 ctgatggagt aactgctaat gcgccgttta gttttgcatc agcttttaat gcacctaaac
     4141 cctcgactaa tactgctgat ggcaaagatt ctgcttcgaa tcttaccact ccttcacctg
     4201 ctttttcatt tggaaacaat agtggtgtta aagcatccag taacaacaat ccctctacaa
     4261 attcgtctac ggcaccgttt tcttttggta catcaaataa accggcattt agctttgggt
     4321 ctgcaacttc aaagactacc tctgaaggaa ccgctccagc tgcttctgct tctgctcctg
     4381 ctcccactac gtctgcattt tcttttggag catcaaattc tagtatgaat aaggaagaga
     4441 acactcccat ggctaaggat gctggagata cggcacctgc gtctggcttt aagtctgggt
     4501 tttcgttcgg tgctaacaat tcgcctcagc ctgcttctat gtttggtaca tcgacgccgg
     4561 ccccgtcttc cgcgtttgcg tttggcaacc agtcaggcac gaacccagca gctcctgcag
     4621 ggtttggagg gattactaat actgccacta ataaccctcc tagtacaggt tttacattca
     4681 ctccatcaaa tgctggatca actgcggctc ctatgtttgg cgctggaaac actcccaacc
     4741 catccgggtc catcaataat gcatcacaag cattcgcctt cgggtcagga gaacctagta
     4801 atccagctag taatccacct agtaccggat tttcgttcgg tgcggctact ccgtctgcct
     4861 ttaatgcaag tgcctcacaa tcaccagcgc ccaatggtat tcaattcaat ttgggctctt
     4921 ctaactcaca aacaaatgcg cccccgggcc gtaaaattgc tgtgccccga agtcgaagaa
     4981 aacgtggatc catgtctaaa ggtgaagaat tattcactgg tgttgtccca attttggttg
     5041 aattagatgg tgatgttaat ggtcacaaat tttctgtctc cggtgaaggt gaaggtgatg
     5101 ctacttacgg taaattgacc ttaaaattta tttgtactac tggtaaattg ccagttccat
     5161 ggccaacctt agtcactact ttcggttatg gtgttcaatg ttttgcgaga tacccagatc
     5221 atatgaaaca acatgacttt ttcaagtctg ccatgccaga aggttatgtt caagaaagaa
     5281 ctattttttt caaagatgac ggtaactaca agaccagagc tgaagtcaag tttgaaggtg
     5341 ataccttagt taatagaatc gaattaaaag gtattgattt taaagaagat ggtaacattt
     5401 taggtcacaa attggaatac aactataact ctcacaatgt ttacatcatg gctgacaaac
     5461 aaaagaatgg tatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggttctgttc
     5521 aattagctga ccattatcaa caaaatactc caattggtga tggtccagtc ttgttaccag
     5581 acaaccatta cttatccact caatctgcct tatccaaaga tccaaacgaa aagagagacc
     5641 acatggtctt gttagaattt gttactgctg ctggtattac ccatggtatg gatgaattgt
     5701 acaaaagatc tggcgcgcca cttctaaata agcgaatttc ttatgattta tgatttttat
     5761 tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt
     5821 aaaacgaaaa ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt
     5881 atagtatgag gtcgctctta ttgaccacac ctctaccggc agatccgcta gggataacag
     5941 ggtaatatac tagtaagatg attcttttat cgtcgataac gatgaggatg tagacgatta
     6001 tgatgagagt gattagcttc taaaatttgg tgttttcaaa cgctatttac agtacaatct
     6061 gaaaaaattg gatgaacatt gtaaatggta ggatacgagt tccaattaat gtatttttta
     6121 agtattacac ttagcttatt aattttatat atacattcta cctctgtggg tttaaagaca
     6181 actcgtacac atactcaaat tatttatgaa cattataatg catattcgta ctgtctatta
     6241 aacatttttt tgacattaaa aatttcgttt actaacgtag aaagcgctga taattgaggc
     6301 tactatttta atatctagaa acaaagattc attaattaat taagtaagct ccaggatact
     6361 tgtatatttc gttaaaaaag cgcaatttca acaattccta tgaacatcca tgatttcatc
     6421 acaaaatcaa acatatcagg atttaacgtg aattatttgc tttcttgtga ttttcatctg
     6481 atttctggtc atttacgtta ctgtaaacaa gctttttaaa aaaaggattt tttacgcaat
     6541 attatcgata tcccaatctg tagtataaaa agtgcaacag tgttgagttt cccgaaacca
     6601 ggcatttttg cggtagggtc tgaaactttc acgaaaccat ttcacaacaa aacttcttca
     6661 acaacatgtg tgcaaagaaa atcgtcgtct taccaggaga ccatattggc cctgaaattg
     6721 ttgcttctgc cttggaggtt ttgaaagtcg ttgagaagaa gcgacctgag ttaaaactcg
     6781 agtttgaaga acacaagatt ggaggtgcct ctattgatgc ctatggaacc cctttgactg
     6841 acgagactgt gaaggcttgt ttggaagctg acggtgttct tttgggtgcc gttggtggtc
     6901 ctgaatggac caaccccaat tgtcgtcctg agcaaggttt attgaagctt cgtaagagta
     6961 tgggtgtttg ggccaacctt cgaccttgca actttgccag caagtcttta gtcaagtaca
     7021 gccctttgaa gcctgaaatc gttgaaggtg tcgatttttg tgttgtacga gaacttactg
     7081 gaggttgtta ctttggtgag cgcactgagg acaacggatc gggttatgct atggacactt
     7141 ggccttacag tttggaagaa gtttctcgta ttgctcgttt ggcggccgcg ggcccaacac
     7201 caatgtttat aaccaagttt tatcttgttt gtctacatgg tattttacat tcatctacat
     7261 acatctttca ttggctttgt acatagttat cattacaagt ctaaaaaaat tcactctttt
     7321 cttattcaat gtcaatccaa gagaaaagat tgtggtaatg ttgtaggagc atgtttaata
     7381 aattactata gcaaattact ttttattccc aaggtgttta tctataatag ttaatatttt
     7441 agtcgctaca taaaatttta ccaaagagta cttgtatact aattctaaat gccttctgac
     7501 ataaaacgcc taggaaaaca aacgcaaaca aggcatcgac tttttcaata accaaccaaa
     7561 aaaattttac attagtcttt ttttaatgct gagaaagtct ttgctgatat gccttccaac
     7621 cagcttctct atatctcttg gcttcgacaa caggattacg accagctcca tagactccac
     7681 gaccaacaat gatgatgtcg ctaccgcagt ttacaatcac ttcttcagga gtacgatatt
     7741 gctgtcccag cccgtctcct ttaacatcca agccgatacc aggggacata gttatgtagt
     7801 cgctttgaag gttaggaaat cgacgaccag ctataaagcc aaagcaaaaa tcggtatgct
     7861 tctcaaacca ttctaaggtt ttctctgtgt aggaaccagt agccaaagag cctttggaag
     7921 acatttcagc caaaagcaag agaccacgtc ccaaaggtaa accaacttct ttgaggcctt
     7981 gtataatacc ctcgcctggc actgtatggc aatttgtgat atgagcccaa gaagcaattt
     8041 tgtacacacc agatgcatat tgtagcttga cggtatttcc aatgtctgcg aatttgcgat
     8101 cctcaaagat aagaaaacga tgctttttac ctaaggccac cagtttttct accatatcct
     8161 ggtcgaaatc ctcgacaacg tcaatatgtg tcttgataac acagacatag ggtccaattt
     8221 tatctaccaa ttctaagatt tcggatttct tcgtcaaatc gaccgcgact gacaagttgc
     8281 tttgcttttc ttccatcaaa gccaacaatt ccttggcaat gggatttttc atcccctcag
     8341 ctctagctga atagctttga aatactctag catccataac tttgctttta aacctttaat
     8401 ttcgatccaa gcaaaaaaga ggttcttggt aggacaatac ggtaagaaaa cacgacatgt
     8461 gcagagatgc cgacgaagca tagttaaact gggatggtaa aatcaattaa gaatttataa
     8521 agacaaaatt gtataagtct ctaaaacatc ttaattatac ctcacagaac tatctaaaat
     8581 atattcacaa agtgcaaaca ttatcatgaa aaagaaccat tttaatttaa agcaagggca
     8641 ttaaggctta tttacagaat ttcttacttt tgtaaagatt ataaggctga ttatcttttt
     8701 caccatgcca aaaattacac aagatagaat ggatgtttga aattaaacgt gagtatacaa
     8761 acaaatacac taggtaaatc gaaacatttt tttctccatt aagtaacaaa ttcctattta
     8821 gagaaagaat gctgagtaga ttaaataatc tatacaaact tttttaacac aaatgcatac
     8881 atatagccag tgggatttgt agctaagctt gcgaattcga gtctaactcc ttaaccactc
     8941 ggacatagtg acttatctga cactcattgt aaattaatat atatataggc attttgttta
     9001 gttaaaggta cttaagtaat tagtataaac gaaccaattt tataatcagg aagttaagtg
     9061 aatggtagca catgtcgtaa aaattgtgaa tttttattga ataatatttt aaatacaagc
     9121 ctttctagac taggtatact cataaacata tatgagcaaa aggatagagg agattacatt
     9181 gcatcttcta caaatttatt tattgccctt tactgaaaaa ttaaataatg agtactaaat
     9241 gataaaaagc gctcagtaca agaaagattg caaaaatatt gcattcttca tgaattaaag
     9301 ttgcatataa ggcatattga aagtaatagt actaaaacag cagttagcga aaattaatag
     9361 aattatattc gcaagacaat tgtgacatta aattaaaaaa ttgtagaatt tttactatcc
     9421 tctttaacgc catgagcctt tataaaaagg ttaaattagt tttaacattc tttttttgag
     9481 taagagttta caatttatca aaacctgtgt tattatattc attagtttca atttattagc
     9541 atctagagaa aaatcaattg gcagttacat tgttggaatt tatgaagaaa aagaatctac
     9601 aacggagaat aagttgctga tcgctttccc taaaattgta tattttgctg agcttatttt
     9661 gacatttcgt tgaagttttc tctaattcgc attcatttta agtaaaacaa tgagaaataa
     9721 aattacaaaa aatacaaatt aaaatacaat ttttagctat aattatagac gatgcccttg
     9781 tatcccattc tgtctcgctt gcccctactt tttatctttt atataccata atgaacgctg
     9841 ccgctactaa ccataccccg attttacatt tcggactccc aaggacgtac aaaatagaaa
     9901 actatagaaa aaaataatca gaaaatagca tgtcatctct ttgtaaaacg cgtttgcaag
     9961 aagaaaggaa acaatggaga agagatcatc catttgtatg taaattttag taaacttgaa
    10021 gaaatcacta acaacttctc ttacttaggg attctatgca aaaccttgta aatcatctga
    10081 tggaggactc gatttaatga attggaaggt tgggcccgcg gccgcttagc tgaaacttcc
    10141 aaccctcctg ctcccgtcac attactcgac aaagctaatg ttttggcaac ttctcgtctt
    10201 tggcgcaaga ctgtagctaa gatttttaag gaggaatatc ctcaccttac cttaaaaaac
    10261 cagctcattg actccgcagc catgcttttg gtcaagagcc ctcgtacact taacggtgtt
    10321 gttttgactg acaacttgtt tggtgacatt atttcagatg aggcttctgt cattcctggt
    10381 agcttgggcc ttttgccttc tgcctccctt tccggtgtgg taggaaaatc agaagaaaag
    10441 gttcattgtt tggttgagcc cattcacggt agcgctcccg agctcgaatt cactggccgt
    10501 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
    10561 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
    10621 acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct
    10681 gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca
    10741 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta
    10801 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
    10861 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
    10921 cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt
    10981 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
    11041 acaacactca actctatctc gggctattct tttgatttat aagggatttt gccgatttcg
    11101 gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
    11161 ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
    11221 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
    11281 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
    11341 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
    11401 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
    11461 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
    11521 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
    11581 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
    11641 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
    11701 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
    11761 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
    11821 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
    11881 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
    11941 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
    12001 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
    12061 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
    12121 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
    12181 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
    12241 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
    12301 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
    12361 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
    12421 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
    12481 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
    12541 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
    12601 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
    12661 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
    12721 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
    12781 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
    12841 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
    12901 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
    12961 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
    13021 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
    13081 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
    13141 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
    13201 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
    13261 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
    13321 cgaacgaccg agcgcagcga gtc
//
