LOCUS       sequence-306516-        1777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018257
FEATURES             Location/Qualifiers
     source          1..1777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1725..1745
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1746..1769
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgaagcgctt cgccatggcg gcctcgctcg tgatgctggc cagtacctac gccaacgcgc
      121 agacgaccga acagcttgtc aagggagcca cggacacgtc gaacgtgctg aactatggca
      181 tgggctacaa cctgcagcgc ttctcgaccc tgaaccagat caataaggat accgtgaaga
      241 atctggtccc cgtctggaat tactctttta atgacgaccg ctccgaagag tcgcaaccgc
      301 tcgtctacca gggagtgatc tacgtgacga gccacaacgc gaccatggcc gtggacgcga
      361 agacgggcaa gcagatctgg aagagcaaga tcgagtaccc ggcggagacc ccgcggatcg
      421 tgtgttgcgg gatcatcaat cgcggcgccg cgctgtacga cgggaaggtg ttccgaacga
      481 cgctggacgc gaacgtcatc gccttggacg ctaaggacgg caaggagctc tggcgccaga
      541 aggcggcgga catcaaggag ggatactcca tgacggtggc cccgctggtc gcggacggcg
      601 tggtgatcac gggcatcagc ggtgccgaat tcgggacccg cgggttcatc gacggctggg
      661 accccgcgac gggcaagcac ctctggcgca cacattcgat cccgagcccg gatgaaccgg
      721 gcggcgatac gtggaagggc gatacctgga agctcggcgg cggctccacc tggatcaccg
      781 gttcgtatga cccggagctg aacacggtgt actggggcat cgggaacccg ggaccgttca
      841 actccgcggt gcgcccgggc gacaacctct acacgtgctc ggtgctggcc atggacccga
      901 agacgggaaa gatcaagtgg cactaccagt tctcgcccaa caaccccttt gactatgaca
      961 gcgtcgcgga gatggtgctt gcggacatga acgtcgaggg taagccgacg aaggtgctga
     1021 tggatgccaa ccgcaacggc ttcttctatg tcctggaccg caccaacggc aagctgctgg
     1081 cggccaaccc gtacgtgaag gtgaactggg cgaccggcgt cgacatgaag accggccgcc
     1141 cgatcgagac agacgtctcg aaggatgccc gcgagggcaa gaaggtgacg gtgtacccga
     1201 gcatcctggg cgggaaaaac tgggaaccga tgtccttcaa cccgcagacg gggcttgcgt
     1261 acgcgaacac gctcgcgttc ggcggcaagt ataaggcgga gccggtcacc ttcaagcagg
     1321 gcgagtggta cctggggatg gatctgacgg atccctggga gttcggcgac ggcgcgcgcg
     1381 gccatctgaa ggccatcgac cccatgaccg gtaaggcgaa gtgggaggcg cccgccgaca
     1441 tcccgcggtt ctcgggtgtg ctgtccacgg ccgggggcgt cgtgttcacc ggcgcgctga
     1501 ccggagaatt cgaggctttc gacgccgaca cggggaaaaa gctctggcag ttccagaccg
     1561 gctccggcat cgagggtcag ccggtgacct ggcagcagga cgacgtgcag tacatcgcgg
     1621 tgacatcggg ctatggtgga gtgtactccc tcttctcggg cgatgaacgc ctcgcgaagg
     1681 tccccccggg cggctcgctg tgggtcttcg cggtgaagca ggccgaaaac ctctattttc
     1741 agggacatca tcaccatcac catcaccact gacagct
//
