LOCUS       sequence-306517-        1780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018259
FEATURES             Location/Qualifiers
     source          1..1780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     regulatory      1265..1274
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1728..1748
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1749..1772
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatataccg
       61 tgctgacctt cggctgcatg cggagggaac gccccgctat ctgtgcggcc gggggggagc
      121 tggcctcttc gggacccgcg cggatcgact ccctcccggc gccgggcgcg gcggccgtcc
      181 cggacgtcga ctggccgacc tacggcggca acgacgccgg ccagcggtac tcgctgctcg
      241 cccagatcga ctccggcaat gtccgccggc tcgtcccggt ctgcctgcac cgcgcccagg
      301 tcggtctgga aacgcagcag agcacccccg tcgtcgatgg tgacgccctc ctctataccc
      361 tcccctacga tgtggtcgtg gccatggacc cccggaccgg ccgggaattg tggcgctaca
      421 cgccgaggct ggcccgggac tccctggtgc tgtgctgcgg cccccacaat cgcggagtgg
      481 ccgtggacgg cgcgcgcgtg tacctcgcca cactcgatgc gcgactggtg gcccttgacc
      541 gtcgcacggg tgcggtggcg tgggaggtgc gggtcgccga gccgatgaag ggctacagct
      601 tcaccgcggc gccgctgctg gccgacggca aggtgatcct cggcactgcc ggaggcgaat
      661 tcggcatccg aggttgggtc gacgcctacg acgcgggcac ggggcagcgc gcctggcgct
      721 tctacacggt gccctcccca gacgagggcg gctggtgggg ccgctgggcc gccaccacgc
      781 cagacggcga ggacctgcat cgcgatctgg cccgggagcg cgccgattcg gctcggtacg
      841 cggatgcgtg gcgcacaggc ggcggacccg tgtggatgac cccggcgttc gacgccgcgc
      901 tcggcctggt gtttatcggg atcggcaatc ccgcccccga tatcgacgaa agcaagcgcc
      961 ccggcgataa cctctacacg agttcgatcg cggccctcga cgtgcgaacc ggggccctga
     1021 agtggtatca tcagctggtg ccgcacgacc ggtgggacta cgacgccgcg aacccggtgg
     1081 tgctcctcga tgccaccgta aacggcgcca ccgtcccggc tatcgcccac gcaggcaagg
     1141 cgggctgggt gcatgtgttg gatcgccgcg atggccgccg actggtgcgg accgaggccc
     1201 tggtgcccca gaagaatctc ttcatgtccg cgacggcgga gggcatcgag gtcgcgcccg
     1261 gcggccatgg cggcgcaaac tgggccccgt cggcttatag cccgcggacc cgtctgatgt
     1321 acgtgctggc caaccacgag ccctcagtgt tcaaactcgt ccccgccccc ctctcgcagg
     1381 gcgcgcagtg gttcggcggg gagatgacgg tggcgaaccg cggccgcggc gcgttcggcc
     1441 gcctcaacgc ggtggatccc tccaccggca agatccgctg gcagctcacc gagaagaacc
     1501 tgaaaaacta cagcgggggt acgctcgcga cggcgggaga cctcgtgttc tttggcgacg
     1561 cggatggcta tctcaacgcc gtcgatgccc gcagcggcgc ccggctctgg cgtcactgga
     1621 cggggcgacc gagcatcgac gccgccccga tgacgttcag tcggggtggc cgccagtacg
     1681 tcgccatcgc gacccagggc gcgctggtgg ccttcaccct ccgcgccgaa aacctctatt
     1741 ttcagggaca tcatcaccat caccatcacc actgacagct
//
