LOCUS       sequence-306520-        1810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018265
FEATURES             Location/Qualifiers
     source          1..1810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1758..1778
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1779..1802
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgaaccgcat cgtgcggaat ctggcgttcg gcggcctgac attgctgctc tgcctcctgg
      121 gcaccgcggg ctgggcacaa gacgatgaac cgagcaagcc ggtcaccgac gtcttgctcg
      181 ccgccgaccc cgccgagagc tggctccata cgaacggcaa ctgggcgggg cagcgctatt
      241 ctacgctctc gcaactgaac gcctacaatg tcaagggcct gaaggtcgcc tggatgttca
      301 gcaccggtgg caagaccgat gcccagaaca cccccctcta ccatgacggc ctgatctact
      361 tcgcccagga caaccaggtc ttcgccatca acgcgcgcag cgggaagcaa gtctggctgt
      421 acacgcatga gcttcccgaa gatttcggag gctacaacgt ccccttcatc acgggcaagc
      481 accgcggggt agcgctctac ggggagcaca tctatttcct gtccaacgac accaaactcc
      541 atgccatcca ctacaagacc ggccagccca agttcgtcaa gcagtacctc aagtacccga
      601 agaagttcga agaggccgag gatgcgaatg gctacgcgac caccgtgggc agcctcgcca
      661 tcccgggtaa gatcctcgtg ccgctcaacg ggacggattg gggcggcctc cccggcttcg
      721 tctacgcggt tcaccccgac aacggggaga tcctctggca ggccaacatg atcccgggcc
      781 cgggcgagcc gggcgccgag acgtggcccg gcgatagccg cgagtacggc ggcgccggcc
      841 cgtggatcac gggctcgtgg gatcccgagc tcaagatgta ctatacgggc accgccaacg
      901 cgtacccctg gaaccctaag acgcgcgggg acgggaagta cgataacgtc ggtgccgcct
      961 cggtggtggc catcaacacg gatacaggca aggtggtctg gcgctacgta gtggttccgg
     1021 gcgacccgtg ggactacgac accatgtcga ccccggtcat catcaagctc gatggcgaaa
     1081 aggtcctggt gcagccgaac aagaccggct acgtgcatta cctggatccg aagaccggcc
     1141 agttcctgcg ggcggtcgca ttcgcggaca agatcaactg ggcgaagggc tatgacgtgg
     1201 aggggcggcc gatcgaccag atcgcgctgc cgaaggaggg tgccgacccc gtggaggtgt
     1261 ggcccagctt gctgggtgcc gtcaacatgt accccacctc gtacagcccg aagacgggcc
     1321 tcatctacct ccccgccgtc aacgctggca tggagtacgg ttacgaggag atcaagacga
     1381 tcagcaacgt tcggcatttc ggcgcctatc aggagttcat ttggggctac gaggtcaatc
     1441 tcgccctaaa cccgcagacc ggccaggagg tctggcggga ccagaagagc aagcacggct
     1501 acgcgggcgg catgctctcg acggcgggta acgtggtgtt ctacacctcc gccagcggcc
     1561 agttccacgc caccaacgcc gccacgggag agatcctcta taccttcaac ctgggaacca
     1621 ccgcgaaggc cggcccgatc accttcatgc tggacggcaa gcagtacgtc gtgcaggccg
     1681 tgggcggagt cccgggcttc ggatacgagg gccacaacct gcagcatggt aacatggtcg
     1741 ttgcgttcac ccgagccgaa aacctctatt ttcagggaca tcatcaccat caccatcacc
     1801 actgacagct
//
