LOCUS       sequence-306521-        1828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018267
FEATURES             Location/Qualifiers
     source          1..1828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1776..1796
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1797..1820
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgacgtttgg gaccaagggc ttcctggcgg gcatctcgat ggtcgccatg ctggcgacgg
      121 cgacctcggg ggtccgtgcc aacgactccc tcctcaaggc tcagagcgac tcgtcgcaat
      181 gggcggtcgc ggggcatgat tacgggaaca cccgattctc cccgctgaag cagatcaaca
      241 ccgagaatgc gggcaaactc accctggcgt acagtttttc gctcgcctcg ctgcgttcca
      301 acgaatcgag ccccattgtc gtgggcaaca cgctctacgt ctcgtcgagc tggggcccca
      361 agtatgtgta cgctctggac gccgcgaccg gtgtacgcaa gtggacctat gagccggaca
      421 ttccggagga cgttctgcag tacgcttgtt gcgacgtcaa caaccgcggc gtcagctacg
      481 ccgacgggaa gatcttcgtc ggccggctgg atggcaaact caccgccctt gacgccgcca
      541 ccgggaaggc cctgtggacc gcgaaggtcg tcgattataa gcagggttcg gtcatcacca
      601 gccccccgct cgtggtgcgc gataaggtta tcaccggttt cggcggcggc gagtacggtg
      661 tccgcggctc cctccaggcc ttcgacatca acacgggcaa acagctgtgg cagacctaca
      721 ccgtcccgag tccggatgag ccgggcggcg acacctggaa gggcgacagc gcccagcacg
      781 gaggcggcgc cgcgtggctc gtcggctcct acgatccgaa aacggacacc gtctactggg
      841 gcacctccaa cccgggtccc tggaataccg ccgtccggtc gacaggcgat ggcaacttcg
      901 gcaagctgac gaacctatac accgcctcca cggtcgcctt cgaccccaac acgggtaaga
      961 tcaagtggca catccagacg acgccggccg acgcgtggga ctatgacggc gtcaacgaag
     1021 cggtcctcgc cgacctcaag atcggcgggt cgaccgtgcc ggcgctgatg aaggccgacc
     1081 gcaacgggta cttcttcgtc gcgaatcgcg agaccggcaa ggtcctctcg gcagaaaagt
     1141 acgtgttctc aaactgggcg aagaagtggg acatcgcgac catgagggcg gtcgaagacc
     1201 ccgacaagcg gccgggcccc aaccatccgg cgaaggacat ctgcccgaac ctgattggcg
     1261 gcaagaactg gcagccgatg tcgttcaacc cacagacggg cctggtttat atcccgagca
     1321 acaacgtctg catggattgg tccgtcagcg acgtcgcgta taagcgcggc gtcttctacc
     1381 tcggggccga gttcccgacg aaggaaggcc cggggggctt cctgggggag ctagtcgcct
     1441 gggatcccgt cgcgcagaag aaggtctgga gcatcaagga ggacctcccc ttcaacgggg
     1501 gcacactgac gacaggcggc gggctggtct tcgccggcaa cattcatggc gatttccgcg
     1561 cgatcgatgc caaatcgggc aaggtcctct ggtcgcggaa cctggggagc ggcatcggcg
     1621 cgggcccggt gacctaccag gttgacggca agcagtacgt ggccatcgtt gtgggccgca
     1681 ccgccgcgct cccggcgttc ttgggcgagg tcggcaagaa gatgacggcc gccgccccag
     1741 agggcggatc cctgtttgtc ttctccgtcc aggccgaaaa cctctatttt cagggacatc
     1801 atcaccatca ccatcaccac tgacagct
//
