LOCUS       sequence-306525-        1858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018275
FEATURES             Location/Qualifiers
     source          1..1858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1806..1826
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1827..1850
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgcagacccc gaagcatggc ggcgcgccca ccgcctccaa tctccatagc ccgcaacccg
      121 aggatggaca gtgggtcatg cccagcgaga actacgacgc gacccggttc tccggtctgg
      181 accagatcga cacccagaat gtccggaacc tgaaggtggc gtggaccttc agcaccggca
      241 tgacccgggg cgaggaggcc gcgcccctgg tggtgggctc cacgatgtac atcgtaaccc
      301 cctacccgaa ctacctttac gccctcgatc tgacgcagaa cggcgcgctc aaatgggtgt
      361 acaagcccgg cgtcgatccg tccagccagg gcgtcgcctg ctgcgacgtc gtgaatcggg
      421 gggcggcgta cagcgccggc aagctgttct ataacaccct cgacaactat accgtcgccg
      481 tcgatgcgca gaccggccgc caggtctgga agacgcagct gggggacatc aataaagggc
      541 agacgatgac gatggtcccc ctggtggtga aggaccgcgt gatcgtcggc aactccgggg
      601 gcgagatggg cgtccgcggc tggctgacgg gcctcgacgc gaagacgggg aagatcgcgt
      661 ggcgcgccta cgccgccggg cccgactcgg acgtgctgat tgggccgaac ttcaaaccct
      721 tctatgatga ggatcgcggg aaggacttgg gcgtcacgtc ctggcccccc ggcgcgtgga
      781 agatcggggg cggcggcgcc tggggctggg tgagctatga ccccgaactt gacctcatct
      841 actacggcac cgcgaaccct gggccctgga acccggacca gcgcccgggc gacaacaagt
      901 ggtcgtgcac cctcttcgcg cgccgcccgg agaccggcga ggcgatctgg gcctaccaga
      961 tcggcccgca cgacgacttc gactacgacg gcgtgaacga gaacgtgctc gtggacctcc
     1021 cgatcaacgg ccagacccgg aaggtgctcc tgcaccctga cagggacggc tacgtctaca
     1081 tgatggaccg cgccacgggc gaggtccttt cggccgaggc gttcggctac atcacgacat
     1141 cgacgggcgt ggacaagaag accggccggc tccagctcgt gaaggccaag tcgccgggat
     1201 tcggcaagac ggtacgggac atctgccccg ccagcccggg cgccaaggac tggcagccgt
     1261 ccgccttcag cttcaagacc ggcctcctct atatcccgca caacaacctg tgccaggagg
     1321 tctcggcgac ggcggcgaac tatatcgcgg gcacccccta cgtcggcgcc accgtgcgca
     1381 tgtacgcggg cccgggggga aaccggggcg agtacaccgc gtgggacccg gtggccggca
     1441 aggtggtctg gaccatcaag gaacgcttcc cggtgtggag cggcaccgtc gtgacgacca
     1501 ctgacgtcgc gttctacggt accatggaag gctggttcaa ggcggtgaat gcaaagaccg
     1561 gccagcagct gtggcagttc aagacgggga gcggcatcat cggccagccc accacctacc
     1621 gcggcccgga cggtaagcag tacgtcgcgg tcctctccgg cgtcggggga tgggctggcg
     1681 ccgtcgtcgc cggcggcctc gacacccgcg acggcactgg cgccctgggc ttcaccaacg
     1741 ccatgatcga cctccctaag tatacgaccc gcggcggcac tctctacgtc ttctccctgc
     1801 ccgccgaaaa cctctatttt cagggacatc atcaccatca ccatcaccac tgacagct
//
