LOCUS       sequence-306527-        1864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018279
FEATURES             Location/Qualifiers
     source          1..1864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1812..1832
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1833..1856
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgaccttccg cgcgaccctg ctcgccgcgg ccggcgccgc ggccctgctc gcgggcgtcg
      121 ccagcccggc actcgccgtg acggacgagg acatcgccaa cgatgccaaa acgaccgagg
      181 acgtcgtcac ctatggtctg ggcaaccagg gccagcgctt cagcccgctt aagacgctca
      241 ctcgcgacaa catcaagtcg ctcgtccccg cctacagctt ctcgttcggg ggagagaagc
      301 agcgtggcca agagtcacag gccctcgtca aggacggcgt gatctacgtg accggctcgt
      361 actcccgcgc gtgggcgatc aactcccgca cgggcgaaaa gatctgggag tacaacgccc
      421 gtctcccgga gggaatcttg ccgtgctgcg atgtggtcaa ccgcggcggg gccctgtacg
      481 acaacctgtt catcttcggc accctggacg cgaagctcgt ggcgctggat gccaagtccg
      541 gcaaagtcgt gtggaagaaa gacgtggacg acttcaagtc cggttttagc ttcaccagcg
      601 cgccgatgat cgtgaagggt aagatcatca ccggggtctc gggcggtgag ttcgggatcg
      661 tcggccgggt ggaggcccgc gacgccaaga cgggggaaat catctggaag cgcccggtga
      721 tcgagggcca cgtgggcgag ctgaacggca agccaagcac gatgaccggc aagacgaacg
      781 agacctggcc cggcgatatg tggaagtacg ggggcggagc cacgtggaac ggcggcacct
      841 atgacgcaga gaccaacctg atctactacg ggacgggcaa tccgggcccg tggaactcgt
      901 ggctgcggcc gggcgacaac aagtggtcgg cctcgcggat cgccctggac ccggacacgg
      961 gcgagatcaa gtgggggttc cagacgaccc cgcatgatgg ctgggactat gatggtgtca
     1021 acgaattcgt ctcgttcgac ctcgtgaagg acggcaagac catcaaggcg ggcgcgacgg
     1081 cggaccgtaa cggcttcttc tacgtcctgg accgcacgaa cggcaagttc atctccgcca
     1141 gtccattcgt gagcaagatc acctgggcga agggcatcga cgagaagggc cgtcctatcg
     1201 aggacccggc cttccgcccc gccgatccgg gaaaggaaaa gcaaggtaaa tccgtgttcg
     1261 ccgtcccgag cttcctgggc ggcaagaact ggatgcccat cggctacagc ccggagacga
     1321 agctcttcta cgtcccgagc aacgaatggg gcatggatat ccacaacgag ccggtggcgt
     1381 ataaaaaagg cgcagcctac cttggcgctg gcttcacgat caagcctatc ttcgaggacc
     1441 atatcgggtc cctcaaggcc atcgacccgg cgaccggaaa gattgcgtgg gagtataaga
     1501 acaaagcccc gctctggggc ggcgtgctct cgacggcggg caacctcgtg ttcacgggca
     1561 ccccggaagg tttcctgaaa gccttcgacg ccaagacggg ggaggaggtc tggaagttca
     1621 acgtcggcac gggggtcgtg gccaccccca tcacctggga gcaggacggg gagcagtggg
     1681 tgggcgtcgc cgccggctgg ggcggcgcgg tgccgctgtg gggcggcgag gtcgccaagt
     1741 cgacggccgg tatcacccag ggcggctcgt tctgggcgtt caagctcccg aaggaagtgg
     1801 ccgcccgggc cgaaaacctc tattttcagg gacatcatca ccatcaccat caccactgac
     1861 agct
//
