LOCUS       sequence-306529-        1870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018283
FEATURES             Location/Qualifiers
     source          1..1870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1818..1838
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1839..1862
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgaccatcaa gtcgttcaag cgctgcggcc tctacgcagc gctgagcctc gcgatgaccg
      121 cgtcgcccgt cttcgccggc gtcacggata aggacatcct ggccgatcag gaaacgatcg
      181 aggacgtcgt gacgtacggg cttggcctcc gcggccagcg ctacagcccg atggacacca
      241 tcaaccgcga gacggtgaag gaaatccggc ccgtctgggc cttctcgctg ggcggggaga
      301 agcagcgcgg ccaggagagc cagccgatgg tcaaagatgg cgtcatgtat atcacgggct
      361 cgtactcgcg agtgttcgcc atcgacgcga aaacgggcga ggagctctgg cagtacgacg
      421 cgcgcctgcc ggacgggatt atgccatgct gcgatgtcat caaccggggg gtcgccctgt
      481 acgacgatct cgtcatcttt ggtacccttg acgcgaagct cgtcgccctg gacgccaaga
      541 cgggcaagac gcggtggaag aagaccgtgg acaactacca agacggctat tcgatcacgg
      601 ccgccccgat catcgtcaag ggcaaggtga tcacgggcgt ctcgggcggt gagttcggca
      661 tcgtgggtaa ggtgcgggcg tatgatgcca agaccggcaa gctcgtctgg gagcggccga
      721 ccgtggaggg ccatatgggc tacgtgtgga aggacggcaa gaagacggag aacggcatct
      781 cgggcggcgc ccccggcaag acctggccgg cggacctctg gaagagcggc ggcgccgccc
      841 cgtggctcgg cggtacttac gatgccgaca ccgacctgct cttcttcggc accggcaacc
      901 cggccccgtg gaactctcac atgcgtccgg gcgacaacct cttctcctcc tctcggcttg
      961 cgattgaccc ggatgacggc aagatcgtct ggcacttcca gaccaccccc catgacggtt
     1021 gggatttcga cggcgtgaat gagcttatcg cgttcgacta caccgagaac gggaagaagg
     1081 tcaaggccgc ggccaccgcc gaccggaacg gcttcttcta cgtcctgaat cgcacgaacg
     1141 gcgacttcat ccgcgggttc ccgttcgtga acgagatctc gtgggctacc ggcctcgacg
     1201 acaagggccg cccgctgtac gtgaaggata accgcccggg caacccgtcg ggcgagaagc
     1261 agggtaagac agtcgaggct gtcccttcgt tcctcggtgg caagaactgg atgccgatgg
     1321 cctactcgcc ggacaccgga ctgttctacg tccccgccaa cgagtggaag atggacatct
     1381 ggaatgagcc cgtcgcctac aagaagggcg ccgcctatct gggggcgggc ttcaccatca
     1441 agacgctaaa cgacgagtac atcggagccc tccgggcgta tgatcccaag accggcgagc
     1501 gcgtgtggga gtacaagaac tacgcccctc tctggggagg cgtcatgacc accgccgggg
     1561 gcctggtctt caccggcaat ccggagggct atctgatggc gttcgatgat aagaccggcg
     1621 aactcctcta caagtttaat acgggcagcg gaatcgtcgg ttcccccgtc acctgggaga
     1681 tggacggcga gcagtacgtg agcgtgctct ccggctgggg cggcgccgtc ccgctgtggg
     1741 gcggggacgt cgccaaacgg gtcaaggact ttaaccaggg cggcatggtc tggacattca
     1801 agctgccgaa gtcggccgaa aacctctatt ttcagggaca tcatcaccat caccatcacc
     1861 actgacagct
//
