LOCUS       sequence-306530-        1870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018285
FEATURES             Location/Qualifiers
     source          1..1870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1818..1838
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1839..1862
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgaagcgctc gaccctcgcc ccgttcatcc tggcggcctt cgcgcactcg ggccccgtgc
      121 atgccggcgt gacgacggcc gatatcgaga acgacgcccg gaccaccggc gacgtcctgt
      181 cgtggggcat gggcacggag ggccagcgct attccccgct caccaagatc aatacgaaga
      241 acgtcaagcg tctggtgccg gtctggtcgt attcgtacgg tggcgagaag cagcgcggcc
      301 aggaggcgca gccgctcgtc gtcaacggca aaatgtttat cacggcctcg tacagtcgca
      361 tcttcgcgct ggacaccaag acggggcgca aactctggtc atacgagcac cgcctgccgg
      421 aaggcattat gccgtgttgc gacgtcatca accgtggtgc cgccgtgtat gataacctgg
      481 tcatctatgc caccctggac gcccagctcg tggcgctgga ccagaacacg gggaaggtcg
      541 tgtggaagga gaagatcgac gactaccagg ccggatactc gtcctccgcg gccccgatca
      601 ttgccaaggg gctggtgctc accggcgtca gcggcggcga gttcggcatc atcgggcgcg
      661 tcgaggcccg ggatgccaag accggaaaga tggtgtgggt gcggccgacg atcgagggcc
      721 atatgggcta caagttcgat gagtctggga acaaagtcga caacggcatc tccggcacca
      781 cgaacgcgag ctggcaaggc gacctctgga agaccggcgg ggcggcgacg tggctcggcg
      841 ggacgttcga cgccaagacc ggcctggcgt acttcgggac cggcaacccc gccccgtgga
      901 attcgcacct ccgccccggc gacaacctct atagctgcag caccgtcgcc atcgacgtcg
      961 ccacgggcca gatcaagtgg cacttccagg gcacgcccaa cgacggctgg gactacgacg
     1021 gcaccaacga gttcgtgacg ttcgacgccg gtgggaagcg cctgggcgcc aaggccgacc
     1081 ggaatggctt cttctacgtc ctcgacgcca atacgggtaa gttcgagcgc ggctttccgt
     1141 tcgtcaagaa gcttgattgg gcgaaaggcc tcgacgagaa cggtcgcccg atctacgacc
     1201 ccgccaagcg gcccggcgat ccgaccgcat ccgaagacgg caagaagggc tccgtggtcc
     1261 atgtctcgcc gtcgttcctg ggcggcaaga accagatgcc gatggcctat agcccggaca
     1321 ccgggctgtt ctatgtcccc gccaacgagt ggtcgatgga gatctggaac gagccgatca
     1381 cgtataagaa gggcgcggcc tacctcggcg cggggttcac catcaagacc gctgacccga
     1441 aggaaatcgg cggcgactat atcggcgcgc tccgagcggt cgaccccaag tccggtaagg
     1501 tggtgtggga aatcacgaac aacgccccgc tctggggcgg cgccatgacg accaaaggcg
     1561 ggctggtgtt ctggggcacc ccggagggct tcttcaaggc cgcggatgcg aagacgggca
     1621 aggagctgtg gtcgttccag gtcggcacgg gcatcgtggc gccccccatc acctgggagc
     1681 aggacggtga gcagtacgtc gccgtcaccg ccggctgggg tggcgcggtc cccctgtggg
     1741 ggggcgatgt tgcgaagaag gtctcctacc tcaaccaggg cgggtccatg tgggtgttca
     1801 agctcatgaa ggacgccgaa aacctctatt ttcagggaca tcatcaccat caccatcacc
     1861 actgacagct
//
