LOCUS       sequence-306531-        1876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018287
FEATURES             Location/Qualifiers
     source          1..1876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1824..1844
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1845..1868
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgaataagca taacggccgc gcgctgaggc cgggtgcgct ctcgtcggtc gcgggctccc
      121 tcctcgccgc gctcctcttg gcccctatgg tggtgcatgc cgaggatccg gagcccccgc
      181 tcggcatcaa tgtgctggag gccaccttca agtggcgccc gaactacgtc tccgacgaca
      241 tgctcatcca cgcggataag gacgcgaaca actggctcca ctacggcaag gattaccagg
      301 ccacgcgctt cagccagctc cgccaagtga cgcaggacaa cgtcaagaag ctcgtcccca
      361 aatggaacct cagcttcggc gtcaatgacg cgcaggatag ccagaccacc gtcgtgaacg
      421 ggcgtatcta cgtgaccgcg agtcaaaaca aggtcttctc catcgacggc ctcacgggcc
      481 gtatgatctg gaagtacgaa cgatcgctgc cgggtgatct cggtccgcgc ctgtgctgcg
      541 aagccgtcaa tcgcggagtc gcggtctata aggacatggt ctacatggcc acgctcgata
      601 cccatgtggt ggcgctcaac aacaccacag gcaaggtggt gtgggaaaag aagctcggcg
      661 attacacctc gggcgagatc tacaccagca tgccgctcgt cgccgacggc aagatcgtgg
      721 tgggcaactc cggcggagac gtgggtgcca acgtcggcaa gatcaccgcg ctggaccctg
      781 ataccggcga gatcgtctgg cagaccacca cgcggccgat cgacgcgaac gatccgaatg
      841 ccaagacctg ggccaacgac tcgtggcgca cgggcggcgg ctcggcctgg ctgaccggca
      901 actatgaagc aaagacgaac accatctact ggggcgtcgg caacccggcc ccggactttg
      961 acccgagcgt ccggaagggc gacaacctgt acacgaactc caccctcatc atggacgccc
     1021 ggacgggcaa gatcaagaac cacttccagt atacgcccaa cgacgcctgg gactacgacg
     1081 ggaacaacga gacgatcctc gtcgatgatg aaaagggccg caaggtgtgg ctgcatgggg
     1141 accgcaacgg ccatctgtac tccatcgatc gcaccaccgg taagtgcaac tgggtggtgc
     1201 ccatcgcccg tgtcaactgg gtcaagtcat ggggcgacaa ctgccggccg atcgtcaatc
     1261 ccgataaagt ccccggctac gacaaggtcg tcaaggatat cgcgcctatc ctcgacggcg
     1321 gcaaggagtg gcatccagcg gcctacagcc cccagacgaa gctcctgtac gtcccgtaca
     1381 tcgatagcag catggacgtg caggccaaga agatggagtg gaaacgcggc gagtggttcc
     1441 tgagctcgaa ggtcctgaaa gcgaacccgt acaccggcgg cattaaggcc ttcaatgcca
     1501 cgaccggcga gctcgtgtgg cagcgcccgc agtcgacgcc ggccaccagc ggcatgctgg
     1561 cgaccgccgg gggtgttgtg ttcgcgggtg acgccgaggg ctggttccat gccatgcgcg
     1621 acgatacagg cgaggccctc tggcgcttca acgtcggcac gggtattcac gggaacccga
     1681 ccactttcac cgtcgacggc aagcagtatg tcgccatcgt ttatggcccg ggcggcggct
     1741 ccctgtggcc gctggtgtac gatgaactgt tcaagcacca gaaccgcggc ggtggcatga
     1801 tgatcttcgg cctggccgaa gccgaaaacc tctattttca gggacatcat caccatcacc
     1861 atcaccactg acagct
//
