LOCUS       sequence-306533-        1879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018291
FEATURES             Location/Qualifiers
     source          1..1879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1827..1847
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1848..1871
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgatcgtgca gaagaagatc atgggcatcc tggtgggcct gggactggcg tccctggcga
      121 gcctggccca ggccgcggac gtcggcaacg tgaccaacgc catgctggac tcgacggcca
      181 agaacccggc gagcgtcctc tccttcggca tggggaccca gggtcagcgc tactcgcccc
      241 tcacccagat caatgacaag acggtcgcga agctggtgcc ggcgtggtcg ttctcgttcg
      301 gcggcgagaa gcagcgtggc caggaaagcc agccgctcat ccacaacggc aagatgttcg
      361 tgacggcgag ctacagccgc attttcgccg tcgacctcaa gacgggcgcg aagctctgga
      421 agtacgagca tcgcctgccg gatggcatca tgccgtgctg cgacgtcgtg aaccggggcg
      481 ccgcgctgta tggcaacctg gtcatcttcg gtaccctgga cgcctatctc gtcgccctgg
      541 accagaacac gggcaagatc gtgtggaagg agaaggtcga cgattacgcc gccggttaca
      601 gcatgaccgc cgctcccctc atcgcggagg gcctcctgct caccggagtg agcggcggcg
      661 agtttggcat cgtgggccgg gtggaggcgc gcaacccgat gacgggcgac ctcgtgtgga
      721 cgcggcccac ggtggaaggc catatgggcc atcgctatga caaagacggc aaggcgatcg
      781 acaacggcat ctcgggcacg gtgaacaaga cctggccggg cgacctgtgg aagaccgggg
      841 gtgcgtccac gtggatgggt gggacctacg atccccagac caagttggcg tatttcggca
      901 ccggcaaccc ggcgccgtgg aactcccacc tgaggcccgg cgacaacctg tactcctcgt
      961 ccaccgtcgc ccttgacgtg aagacgggcc agatcaagtg ggcctaccag aacaccccga
     1021 acgacgcctg ggacttcgac ggtgcgaacg agttcgtcac ctttgacatg gacgggaagc
     1081 gctacggcgg caaggccgac cgcaacggct tcttctacgt catcgacgcg agcacgggca
     1141 agctccagaa cgcgttcccg ttcgtcaaga agatcacgtg ggccagctcg atcgacctta
     1201 agaccgggcg cccgaactac atcgcggccg gccgcccggg cgaccccacc aagggagagg
     1261 agggcaagaa gggcacaacg gtgttcgctg cccccgcctt cctgggcgcg aagaaccaga
     1321 tgccgatggc gtactcgccg cagaccaagc tcttctacgt gccggccaat gagtggggca
     1381 tggacatctg gaacgagccc atttcctaca agaagggcgg cgccttcctt ggcgccggct
     1441 tcacgatcaa gcccctgttc gacgactaca tcggggccat gcgcgccgtg gacccaaaga
     1501 ccggcaagat cgtgtgggag atcaaaaaca acgccccctt gtgggggggc gtcatgagca
     1561 cagcgggcaa cctcgtcttc tacggcacgc cggaaggttt cctgaaggcg gtggacgcga
     1621 agactggtaa ggagctgtgg aagttccaga ccggcagcgg ggtcgtcgcc ccgccggtca
     1681 cctggcagga cggcgatacg cagtacgtgg cggtggtgag cggctgggga ggtgcggtcc
     1741 cgctctgggg cggcgaggtg gcgaagaagg tgaatttcct cgaacaaggc ggctcggtct
     1801 gggtgttcaa gctggcgtcc aaggccgaaa acctctattt tcagggacat catcaccatc
     1861 accatcacca ctgacagct
//
