LOCUS       sequence-306536-        1891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018297
FEATURES             Location/Qualifiers
     source          1..1891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     regulatory      58..67
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1839..1859
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1860..1883
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tggcggccct gacgctcatc ctcgcggcgg cagtgtcacc cgccatcgcg cagcagcagg
      121 gaagcctgcc ggagcttctg aacctgcaac gcgatgacgg gcagtgggtc atgccggcca
      181 aggattactc ggcgacccgg tatagttccc tggaccagat caacatcctc aacgtggacc
      241 agctgaaggt cgcctggacc ttcagcaccg gggttctgcg cggccacgaa ggcgcaccgc
      301 tcgtggtcgg ctcgaccatg tacctcatca cccctttccc gaacatcgtc tatgctctga
      361 accttacgca gccgggcccg gccgtcaagt ggaagttcgt gccggacaca gacccgcgct
      421 ccatcggcgt cgcctgctgc gacgtggtga accgcggcct cacgtacgcc gatggcaagg
      481 tcttcttcaa caccctcgat gcccacacgt atgccctgaa cgcgaacacg ggcgaggtcg
      541 tctggcaggt taagcaaggc gacgtggccc gcggcgagac catcaccatg gccccgctcg
      601 tggtcaagga caaggtcatc acggggatca gcggcggcga gtttggcata cgcggcttcc
      661 tcacggccaa cgacatccac acgggcgagc aggtgtggcg cgcctacacg accggcccgg
      721 acagcgaggt cctgatcggt gacgacttca agcccttcta cgattcgctc aagggcaaag
      781 atctcggagt gacgacatgg ccggaggacc agtggaaaat cggcggctcg gcgacctgga
      841 cgtggctgtc gtacgacccg gagctggacc tcctgtacca cggcgtcggc aaccccggga
      901 cctggaaccc ggacctcagg cccggcgata acaagtggtc gatgacgatc ttcgcgcgcg
      961 acccggatac gggcagcgcg cgttgggctt accaaaagac cccccacgac cagtgggact
     1021 acgacggcgt gaacgagaac atcctggtca cgctgaccat caatggcaag ccccgcaaag
     1081 tcctggttaa cttcgatcgc aacggcttcg cctacaccat cgaccgcgcg accggcgagg
     1141 tgctggtggc ccagccgtac gtgtatgtca actgggccag cggcgtggat ctcgagacgg
     1201 gcgtgccggc ccgcaatgcg gacaagtcga cgaagcaggg cgtcaacacc accaatatct
     1261 gcccggcggc catggggggc aaggaccagc agccggccgc gttctcgccc cgcaccggcc
     1321 tcttctacgt gccgaccaac aacttgtgta tggattatga gggcaccgaa gtcgcgtacg
     1381 tcgcgggcgt gccgtacgtg ggggccacgg tcaagatgta cgccggcccg gggggcaacc
     1441 gcggggaatt cgtggcgtgg gacggcgccc gcggccgcaa ggtctggggt atcaaggaga
     1501 acttctcggt ctggtctggc gtgctcgcca cgggcggcaa cgtcgtcttc tatggcacga
     1561 tggatgggtg gtttaaggcc gtgaacgccg tcaccggcga ggtcctctgg accttcaagg
     1621 tcgggtcggg catcatcggc gcgccgatga ccttcctggg cccggatggc aagcagtacg
     1681 tcgcggtgta ctccggcatc ggcggctggt cggggctgac cgtggccggc gacctgagcc
     1741 tcgaggatcc gaccgcggcc ctgggcgcgg tgaacgcctt ctcggacctg ggccgctaca
     1801 ccaacaaggg cggcatgctc tacgtcttca gcctcgccga aaacctctat tttcagggac
     1861 atcatcacca tcaccatcac cactgacagc t
//
