LOCUS       sequence-306537-        1894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018299
FEATURES             Location/Qualifiers
     source          1..1894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1842..1862
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1863..1886
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgcgccggtc gatctcagtg gcgagcatcc tgctggtcgc cgtcaccttc gcgatcgcct
      121 acgcgaaccc ggagctgatc cggctccaga ccgacgacaa gcagtgggtg atgccgggaa
      181 aggactatag cgcgtcgcga ttcagcacac tgacccagat caccaaggag aacgtgaaaa
      241 acctgcggcc ggcctggacg ttttccacgg gcacgctccg cggccacgag ggggcaccgc
      301 tggttgtggg cgcaaccatg tacgtccaca cctccttccc caataacgtc ttcgccctcg
      361 atctgaaggg cccggatgtg gggcgcatca agtggaagta cctgccgaag caggatccct
      421 cggtgcaggc agtggcctgc tgcgacaacg tcaaccgggg cctgagctac gcggacggca
      481 agatctttct cagccagctg gacggccatc tggtggcgct ggactccgcc acgggccgcg
      541 aggtctggaa ggcgcagaac gccgacccga aggtcggcgc cacgatgacg tccgcacccc
      601 atgtcatgaa ggacaaggtc atcgtcggcg tctcgggcgg ggagttcggg gtccggggcc
      661 acgtcaccgc atacgatatc aagacgggcc agcaggcctg gcgcgcgtac agcatgggtc
      721 ccgacgagga ggtgctgctg gcggacgact tcaacgcggc gaacccgcac ttcggccgcc
      781 agaaccaagg cgtggccaca tggcagggcg atcagtggaa gacgggcggc gggacgacgt
      841 ggggctggta cgctttcgac cccagcctca atctgatgta ctacggcacc ggtaatccgt
      901 cgacgtggaa tccgtcgcag cgcccaggcg acaataagtg gagcatgacc atcttcgcga
      961 gggacgtgga tacgggcaag gcgaagtggg cctaccagat gaccccctgg gacgcgtggg
     1021 actatgatgg ggtcaacgag atgatcctgg cggatctgac cgtcaagggg cgaccggtca
     1081 aggccgtcgt ccatttcgac cgaaatgggt tcgcctacac cctggaccgg accaacggca
     1141 ccctgctcgt cgcggaaaag ttcgacccgg acgtcaactg ggccacccac atcgacaagc
     1201 agaccggccg ccccgtcgag gtcccggcca tgcggacaca ggccggcaag aacagccagg
     1261 gggtctgccc ggccgccctg ggcacgaagg accagcaacc ggccgcctac agcccccgca
     1321 ccaagctgtt ctacgtcccg accaaccatg tgtgcatgga ttacgagccg ttcgaggtga
     1381 agtacacggc cggccagccg tacgtggggg cgaccctccg catgtttccg ggccccggag
     1441 gccacatggg cgccttcatt gcctgggatg ccgcaaacgg caagatcgtc tggtcgaaca
     1501 aggagcagtt cagcgcgtgg tccggagcgc tggttaccgt cagcgacgtg gcgttctatg
     1561 gaacgctcga aggctacctc aaggccgtcg atgcccgcac ggggaaggaa ctgtggaagt
     1621 tcaagacgcc ctctggcatc atcggcaacc cgatgaccta tatcggaccc gacgggaagc
     1681 agtacgtggc gatcctcagc ggggtcggcg gctgggcggg gatcggtctg gccgcgggcc
     1741 tcgagggaga caccgagggc ctcggcgccg tcggcgcgta caaaaaactc gcggagtaca
     1801 cgaacctggg cggcgtgctg accgtgttca gcttgcccgc cgaaaacctc tattttcagg
     1861 gacatcatca ccatcaccat caccactgac agct
//
