LOCUS       sequence-306538-        1900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018301
FEATURES             Location/Qualifiers
     source          1..1900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1848..1868
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1869..1892
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatataccg
       61 tgatcggccg ctggcgtacg tgggccatcg ccgcggtcac cgtcgcgctg gtctcggtcg
      121 gcgccgcggc cgaccaaaag ggcgagtgga ccatgaccgg gcgcacgtat gacctccagc
      181 gcttctcgcc gctggaccag attaccgctt ccaacgtcgg aaccctcgtc ccgacgtgga
      241 ccttctcgac gggcgccctt cgcggtcatg agggcaaccc ccttgtgatc ggcaacgtca
      301 tgtacgtgca ttccgcgttc cccaacaccg tctatgccct ggacctcacc aaggccggcg
      361 ctcccatgct ttggaagcac gtccctgccc agccggcgga gaccatcccg gtcgcgtgct
      421 gcgacgtcgt gaaccggggc ctcgcgtacc acccgtcggg caagctcttc atcgagctcc
      481 tcgccgggga tctcctggcc ctagacgcga agacgggcaa gcagctgtgg cgcgtgaaga
      541 atgcggatta caaggaggga tcgaccatga ccaacgcccc gatcgtcatc aaagacatcg
      601 tcatcgccgg catctccggg ggcgagttcg gcgtccgggg gcgcgtcacg gccttcaacg
      661 tcaacaccgg ccaggaggtg tggcgcgcct acagcacggg ccccgactcg gaggtcaaga
      721 tcggctcgga cttcatcgct aattacccct cgcacaaggg ccccgacctg ggcgtgacca
      781 cctggcaggg tgagcagtgg aagcgcggcg gcggcacgac ctgggggtgg tactcctacg
      841 acccggagct caatctcttc tactatggga cgggcaaccc cggcacgtgg aacccggacc
      901 agcgcccggg cgacaacaag tggagcatga cgatcttcgc ccggaacccc gacaccggca
      961 tggcgcgctg ggcgtaccag atgacccccc atgacgagtg ggactatgac ggcgtgaacg
     1021 aaatgatcct cgccgacctg aacgtcaacg gcaagaagac gaaggccctc gtgcacttcg
     1081 atcgcaacgg attcgggtac accctcgatc ggaccaccgg caaggtgctg gtggccgagc
     1141 acttcggccc gggcgtcaat tgggcaagca aggtcgatct cacgaccgga gtccccgtac
     1201 gcgacagcaa gtacggcacc acgagcaagg agaatacgac cggcatctgc ccggcggcca
     1261 tcggctacaa ggaccagcag cccgccgcct tctcgccgaa aacgggcttt ttctacgtcc
     1321 ccacgaacaa catctgcatg gattatgagg gcgtcgaggt taaatacgcg gtgggccagc
     1381 cgtacgttgg ggccatcgtg cggatgttcc ccgggcccgg cggaaatcgc gggcgcttca
     1441 tcgcctggga cgcgacgaag ggcaaggtcg tctgggagat caaagaacag ctcgcagcgt
     1501 atggcggcgc actggccacc gccggaggcg tcgtgttcta cggcaccatg gagggttggc
     1561 tcaagggcgt cgacgcccaa tccggcaagg agctctggaa gttcaagacc ccctcgggca
     1621 tcatcggaaa cccgatgacc tatctgggcc cgaacgggcg ccagtacgtc gccgtcctgt
     1681 ccggcgtggg cgggtgggcg ggcatcggcg tggctgcagg catcggccct gaggatccca
     1741 ctgcgggcct cggcgcgctc ggcgcgttcg gtgacgcgga caaattctcc acccaggggg
     1801 gcgtgctgac ggtcttcggc ctcccggaga agctggcctc ccgggccgaa aacctctatt
     1861 ttcagggaca tcatcaccat caccatcacc actgacagct
//
