LOCUS       sequence-306540-        1912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018305
FEATURES             Location/Qualifiers
     source          1..1912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1860..1880
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1881..1904
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgcggcaggc acacctcctg ggcctgatgc tcgccctggg caccaccggg gccctggcga
      121 acgaggacgt gctgaagcgc acgcaggacc ccaaccagca ggtcctccag accctggatt
      181 acgctaacac ccgctacagc aagctcgacc agatcaacgc gtcgaacgtg aagaacctgc
      241 aggtggcctg gaccttctca accggtgtgc tccgcggaca tgagggctcg ccgctggttg
      301 tcggcgacat catgtacgtg catacgccgt tcccgaacat cgtgtacgcg ctggacctga
      361 acaacgacag caagattctg tggaagtacg agccgaaaca ggatccctcg gtgatcccgg
      421 tgatgtgctg cgacaccgtc aaccgcggcc tggcctacgc ggatggcgcc attatcctgc
      481 atcaggccga cacgaccctc gtctccctcg acgctaaaac cggcaaggtg aactggtcgg
      541 tgaagaacgg cgactccaag gtgggggaga ccaacaccgc caccgtcctc cccgtcaagg
      601 acaagatcat tgttgggatc tccggagcgg agtacggcat ccggggccat atgacggcct
      661 acgacgcgaa gaccggcaag cgagtctggc gggcctatag cgtcgggccg gacgacgaga
      721 tgctggtgga ccccgaaaag acgacgtcgc tgggtaagcc catcggaaag gattcctcgc
      781 ttaagacctg ggagggcgac cagtggaaga ccggcggcgg tgcgacgtgg ggctggtata
      841 gctacgatcc aaagctcgac ctgttctatt atggcaccgc gaacccgtcg acgtggaacc
      901 cgaagcagcg gccgggggat aataagtgga cgatggccat cttcgcgcgc aacccggaca
      961 ccggtcaggc caagtggatc taccagatga ccccccatga cgaatgggac tacgacggga
     1021 tcaacgaaat gatcctcacc gaccagaagg tagacgggaa ggagcggccc ctgctgacgc
     1081 acttcgaccg aaacggcttc gcctacacgc ttgatcgcgc gaacggcgag gtcctcgtcg
     1141 ccgaaaagtt cgacccggtg gtgaactggg cgagcaaggt ggatctcgat aagggctcga
     1201 agaactacgg ccgccccctg gtcgtgtcga agtactccac ggaccagaac ggcgaggacg
     1261 tgaactcgaa gggcatctgc ccggccgcgc tcgggaccaa ggatcagcag ccggcggcct
     1321 tcagcccgaa gacccagctt ttctacgtcc cgacgaacca cgtgtgcatg gattacgagc
     1381 ccttcaaggt gacctacacc ccgggccagc cgtacgtcgg cgcgacgctg tcgatgtatc
     1441 ccgcgccggg cggacatggg ggcatgggga acttcatcgc ttgggacaac atctcgggca
     1501 agatcaagtg gtcgaacccg gagcagttta gcgtctggag cggcgcgctc gcgaccgccg
     1561 gcgacgtggt gttctacggg acgctggagg gttacctgaa ggcggtcgac agcaagacgg
     1621 gcaaggaact gtacaaattt aagaccgcct cgggagttat cggcaacgtc atgacctaca
     1681 cccacaaggg caagcagtac gtcggggttc tctcgggcgt tgggggctgg gccggcatcg
     1741 gcctggcggc cggcctgacg gacccaaacg ccggcctcgg ggcagtgggg ggatacgccg
     1801 cgctctcgca gtacacgaac ctcggtggcc aactgacggt cttcgccctt ccgaacgccg
     1861 aaaacctcta ttttcaggga catcatcacc atcaccatca ccactgacag ct
//
