LOCUS       sequence-306541-        1915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018307
FEATURES             Location/Qualifiers
     source          1..1915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1863..1883
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1884..1907
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgcgcaccat ctacgccctc acgtccgtca tcgccctttc gacgggtacg gccgccctgg
      121 cgaatgaggc cctgcaacaa atgatggacg accccacgca gtgggcgatc cagatgggcg
      181 actacagcaa ccatcggtat tcggaactcg accagatcaa cgccgacaac gtgggcgacc
      241 tcaaagtggc ctggacgttc tcgaccggcg tcctccgggg ccatgagggt tccccgctgg
      301 tcatcgggga tacgatgtac gtgcacaccc ccttcccgaa catcgtgtac gcgctggacc
      361 tgaacgacaa cggcaggatc aagtggcgct acgaaccgaa gcaagacacc tccgtcatcc
      421 ctgtcatgtg ctgcgatacc gtgaatcgtg gcgtggcttt cgcggacggc aaaatcattc
      481 tccaccaggc cgacaccacc gtcgtcgcgc tcgacgccaa caccggcgag gtcctttggt
      541 ccgtcaagaa cggcgatccg gaaaagggcg agacgaacac cgcgaccgtt cttccggtca
      601 agaataaggt cctggtgggc atctcgggcg gcgagttcgg cgtccgcggc tccgttaccg
      661 cgtacaacct cgagaccggc gagcgtgagt ggcgcgccta ctcgatgggc ccggatagcg
      721 acacgcttat ggacccggag aagacgacca tgctgggcaa gccggttggc gtcgacagcg
      781 gcaccgccac gtgggaaggt gaccagtgga agatcggtgg tggcaccacg tggggctggt
      841 atagctacga ccccgagctc aacctggtct actatggcac gggcaacccg tccacctgga
      901 acccgtccca gcgcccgggc gacaaccgtt ggtcgatgac gatcatggcc cgcgatcccg
      961 acaccggcat ggccaagtgg gtgtatcaaa tgacccccca cgacgagtgg gatttcgacg
     1021 gcgtcaacga gatgatcctc accgaccagg agatcgacgg ccagatgcgg aagctcctga
     1081 cgcatttcga ccgcaacggc ctggcgtaca ccttggaccg cgagtcgggt gagctgctgg
     1141 tcgccgagaa gtacgacccc gccgtgaact ggaccaccgg cgtcgacatg gatcccaata
     1201 gcgataccta tggtcgccca gcggtcgtcg cggagtactc gaccagccag aacggcgagg
     1261 atgagaacac caccggcatc tgcccggcgg ccctcggcac gaaggaccag cagcccagca
     1321 gctattcgcc caaaaccggg ctcttctacg tccccaccaa ccacgtctgc atggattacg
     1381 aaccattccg cgtgtcgtac accgccggac agccgtatgt gggcgccacg ctcagcatgt
     1441 acccggcccc ggactcgcac ggcggcatgg gcaacttcat cgcctgggac aacatcaagg
     1501 gagagatcaa gtggtcgatc ccggaacagt tcagtgtctg gtcgggagcc atggccacgg
     1561 cgggcgatat cgtgttctac gggaccctgg aaggctacct caaagccgtc cgggccgata
     1621 ccggcgagga gctgtatcgg ttcaagacgc cctccgggat catcgggaac gtgatgtcgt
     1681 acgagcacgg cggcaagcag tacgtggcgg tcctgtccgg cgtcggcggc tgggccggca
     1741 tcgggctggc ggccgggctc accaacccaa atgacgggct gggggccgtg ggcgggtatg
     1801 ccgccctctc cgattacacc gccctcgggg gccagctgac cgtcttcacg ctcccggacg
     1861 ccgaaaacct ctattttcag ggacatcatc accatcacca tcaccactga cagct
//
