LOCUS       sequence-306544-        1915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018313
FEATURES             Location/Qualifiers
     source          1..1915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1863..1883
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1884..1907
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgtcgtcgaa gatgaaacaa ctgccgctct gggtggcagc gctggccatc cccggcaccg
      121 cgctggccaa cgcggacgtc gcgaagctca tcaaggaccc gaagaactgg gctatgcagg
      181 caggcaactt cgagaaccag cggtactcga ccctcaacca gattaacaag agcaacgtta
      241 aaaacatgaa ggtcgcctgg accttcagca cgggcgtgct gcggggccac gagggcggcc
      301 ccctggtgat cggcgatacc ctcttcgtcc acagcccgtt ccccaacaag gtcttcgcca
      361 tcgacctcga gacccagaag atcaagtgga agtatgagcc gaagcaggac ccctccgtga
      421 tcccggtcat gtgctgcgac accgtgaacc ggggcctcgc ctacgcggaa ggcaaagtct
      481 tcctccagca ggcggacacc atgctcgtgg ccctggacgc caagtcgggc aagaagctgt
      541 gggaggtcaa gaacggcgac ccgaaggttg gcggaaccaa cacgaacgct ccccacgtct
      601 ttaaggataa ggtcatcacg ggcatctccg gcggtgagtt cggcatccgg ggctatgtcg
      661 ccgcgtacga catcaacacc ggcaagcagg tgtggaaggg ttattcgacc ggccccgatg
      721 cggagatgct gatggacccc gaaaagacca tgacctggac gaatgggaag atggcgcccg
      781 tcggcaagga ctcctcgctg aagacctggc agggtgatca gtggaagatc ggcggtggaa
      841 cgacctgggg ctggtactcg tacgatcccc aggagaacct gatgtattat ggctccggaa
      901 acccgtcgac ctggaacccg tcgcagcggc cgggcgacaa caagtggagc atgaccctgt
      961 gggcgcgcga tgtggacacc ggcaaggcgc ggtgggtgta ccagatgacc ccccacgacg
     1021 agtgggactt cgacggcatc aacgaggtca tcctcgccga tgtcgatgtg aagggcaaga
     1081 agcggaaggt cgccgtccac ttcgaccgca acggcttcgc ctacaccatg gaccgggtgt
     1141 ccggcgagct gctcgtggcg gagaagtacg accccaaggt gaactgggcg acgcatgtcg
     1201 acatgaacac cgggcgcccc caggtcgtgg ccaagtactc gacgcgccag aacggcgagg
     1261 atgtgaatac caagggggtg tgccccgccg cgctgggcac gaaggatcag cagccggcgg
     1321 cgttcagccc aaagaacggc ctgttttacg tcccgaccaa ccacgtctgc atggactatg
     1381 agcccttccg cgtcgagtat accgcaggtc agccctacgt gggcgcgacc ctctcgatgt
     1441 tcccggcccc gggcagccat ggggggatgg ggaatttcat cacctgggac gccggcaagg
     1501 gaaagatcgt ccagagcaag ccggaaaagt tttcggtctg gtccggcgcc ctcgtcacgg
     1561 ccggcggcat cgcgtgctac ggcaccctcg agggctacct caagtgcgtc gacgctgatg
     1621 acatcaacaa ggaactcttc aagttcaaga cccccagcgg catcatcggg aacgtcaaca
     1681 cgtgggagta caaggggaag cagtacatcg gcatcctgtc gggcatcggc gggtgggcgg
     1741 gtatcggcct ggctgccggg ctcgagaagc cgaccgacgg cctcggggcg gtcggcggct
     1801 acgccgagct cgcgtcgtat accgagctgg gcggaacgat gaccgtgttc gccctcccgg
     1861 ccgaaaacct ctattttcag ggacatcatc accatcacca tcaccactga cagct
//
