LOCUS       sequence-306545-        1915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018315
FEATURES             Location/Qualifiers
     source          1..1915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1863..1883
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1884..1907
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgaaactccg gaccgtctcc ctggccatcc tcgccttgat catgtcgggg atggagctgg
      121 ccgcggccga cgaactcctc gagatgcaga agaactcgga taactggatc atgcccgctg
      181 ggacgtacga caatcagcgg tactccaagc tcgaccagat caacaccgat aacgcgcagg
      241 atttgcgggt ggcctggacg ttcagcaccg gagtcctgcg cgggcacgag ggcaacccgc
      301 tcgtgatcga cggcaccatg tacgtccaca ccccgttccc gaacaaggtc ttcgccctcg
      361 acctggacga ggacggcgcc ataaaatgga agtacgagcc ggtgcagaac tatgaggaga
      421 ccgtcccggt catgtgctgc gacacgatca accgcggcct cgcctatggt gacggcaaga
      481 tcttcctggc gcaggccgac accacgcttg tcgcgctcga tgccaagacc ggcaaggtcg
      541 tgtggtcggt caagcgggac gatccgaagc agggtgccac gagcaccggg gccccccacg
      601 tctttaagga caaggtcctc gtcgggatct cgggcggtga gttcggcgtc cggggctacc
      661 tggaagccta cgacgtgaac tcgggcgagc ggatctggaa ggcctactcc accggccccg
      721 acgaggaaat gctcatcgac ccggaaaaga ccacgcacct cctcaagccg gtcggaaaga
      781 actcgtccct cgagagctgg gagggcgagc agtggaagat cggcggcggc acgacctggg
      841 gctggtttgc gtacgacccc gagctcaacc tcgtgtacta cggcacggcc aaccccggca
      901 cctggaaccc ggtgcagcgc cccggcgaca atcgctggtc gatgaccata ttcgcgcgcg
      961 acctcgacac cggagttgcc aaatgggcgt atcagatgac cccgcacgac gagtgggact
     1021 acgacggcgt caacgagatg gtgctcgtgg accagaagat cgacggcgag gagaggaagc
     1081 tcctggtcca cctggaccgc aacgggtttg gctacacgct cgaccgtgtc accggggagc
     1141 tcctggtggc tgagaagttc gacccggtgg tcaactgggc gacccacgtg gacatggaga
     1201 ccggccgccc ccaggtcgac ccgcgctact caacccacca gaacggccag gatgtgaata
     1261 ccacgaacat ctgccccgca gcgctcggct ccaaggacca gcagccggtt gccttcagtc
     1321 cgcggaccgg cctcttctac gtccctgcga atcacgtctg catgaactat gaaccgttcg
     1381 aagtgagcta taccgccggc caaccctacg tcggtgcgac gcttaccatg ttcccgggcg
     1441 gcaaggtgtc gggcgatggg tcgacccacc tgggccgctt cttcgcctgg gacgcgcgga
     1501 agggcaaggt ggtgtgggaa atcccggagc gcttctctgt ctggagcggc accctcgcca
     1561 ccgcgggcga tatcgtcgcg tatgggaccc tggaaggcta cctcaaagtc gtcgacgcga
     1621 agagcgggcg cgagctttat cgatttaaga cccccagcgg catcatcggc aacgtgatga
     1681 cgtaccagca caagggtaag cagtacctcg ccgtgctgtc cggcctgggc ggctgggcgg
     1741 gcatcggtat ggccgctggg ctccaggggg agaccgaggg cctcggggcg gtcggggcct
     1801 accggagcct ggaggattgg accaacctgg ggggcaccct taccgtcttc acccttcccg
     1861 ccgaaaacct ctattttcag ggacatcatc accatcacca tcaccactga cagct
//
