LOCUS       sequence-306546-        1915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018317
FEATURES             Location/Qualifiers
     source          1..1915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1863..1883
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1884..1907
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgaaccaacg ccatgtcacg ggctttatcg tcgcgctcgc tatcgtcggc atgctcctgg
      121 cgtcaggggc gatggccaac gatgagctga agcgcctgca gaaggatccg gggcagtggg
      181 tcatcgcgcg cggcaactac gccggctggg gctactcccc gctcaagcag atcaacaccc
      241 tgaacgccaa gaagctccgc gtcgcctgga ccttcagcac cggtgccctt cgcgggcacg
      301 aaggggcccc actcgtggtc gggaacacga tgtacgtgca cagcgcgtac cccaaccacg
      361 tgtacgccct tgacctcacg aagcagccgt acgagattaa gtggcgctat acgccgaagc
      421 aggacccgcg cgccgtgccg gtcgcctgct gcgacgtcgt ccaccgcggc ctgaactacg
      481 ttgagggcaa gatcctcatg atcaccctgg acggccaagt catcggactc gacgtcaaca
      541 ccgggaagga gctttggaag gccaagaacg ccgatcccac caagggggcc acgatgaccg
      601 gcgccggcat cgtgatcaac gataagttta ttgtgccggt ctcgggcggc gagttcgggg
      661 tgcggggtca cgtcaccgcg tacaatatca acacgggcga gcgcgtgtgg cgggcctact
      721 ccaccggccc cgacgaggag ctgctgctgg cggacaattt caacgaggcc caccccgaat
      781 acgggcgctt cggcgagggc acgaagacct ggccgggcga ggagtggaaa acgggcggcg
      841 gcacgacgtg gggctggtat acgtacgacc cggagctaca tttgttctat tacagcacgg
      901 gcaacccggg gacctggaac ccgagtcaga ggaaggggga caataagtgg tccatgacga
      961 tctgggcccg cgatccggat actggcaagg cgacctgggc gtaccagatg accccctggg
     1021 atgcctggga ctatgacggc gtcaacgaga gcgtcctggt cgatctgccg ttcaacggta
     1081 gtaccaagaa ggccctcgtc cacttcgacc gcaatgggtt cggctacacc atggaccgga
     1141 ccgacggcac cctgcttacc gtcgagccct tctcctacgt caactgggcg accggaatcg
     1201 acaagaagac cggtcggccc atcgaggatc cggagaagcg cacccgcgcc ggcgaggata
     1261 tcaagaacat ctgccccagc gcgatgggcg gtaaggacca gcagccgtca gcctactccc
     1321 cgcacacggg cctgttctac gtgcccacga atcacctgtg catggactac gagggggtcg
     1381 aggtcaagta cacggcgggc gccccgtacg tgggcgccaa cgtgaagatc tacggcggcc
     1441 cgggcggcca ccacggcgag ttcctcgcct gggatccggt gcagggcaag cgggtctggg
     1501 gtatcaagga acagttcccg gtctggtcgg gggtcctgac gacgggcggc aacgtcgtgt
     1561 tctatggtac catggatggc tggttcaagg ccgtcaacgc gcataccggc gaggtgctct
     1621 ggaagaacaa ggttggctcg ggcatcatcg ccgcgccgat gtcctatatc ggaccctccg
     1681 atggcaagca gtacgtggca gtgtactccg gcgtcggtgg ctggttcggc ctcccgatcg
     1741 ccgcgaacct ccccccgacg gacccgtacg gcgcgctggg cgccgtcaac gcggtccaga
     1801 ccagcggtct ccataaggcc accaccctcg gcggtatgct ctatgtctac tccatcgacg
     1861 ccgaaaacct ctattttcag ggacatcatc accatcacca tcaccactga cagct
//
