LOCUS       sequence-306548-        1918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018321
FEATURES             Location/Qualifiers
     source          1..1918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1866..1886
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1887..1910
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgaagcggtt gctcaccatg ctggcgatca tgtcgatcgg cggcggggcg caggtcgcgt
      121 tcgcgaacga cgagctccag aagctcatag acgatccgaa ccagtgggcc atccagaccg
      181 gtgactacgc caacctgcgc tatagtaagc tggaccagat caacaaggac aacgtcggga
      241 agctgcaggt cgcgtggacg ttctcgacgg gggtgctgcg cggacacgag gggtcgccgc
      301 tggtgatcgg tgacctgatg tacgtgcaca cgccgttccc gaacaccgtc tacgccctgg
      361 atctctcgaa ggacgggcag atcgtgtgga agtacgagcc gaagcaagac cccaacgtga
      421 tcccggtgat gtgctgcgat accgtgaacc ggggtgtcgc ctacgccgac aacaagatct
      481 tcctgcacca ggccgacacg acggtggtgg cactggacgc aaagaccggc aaggtgattt
      541 ggtcggtgaa gaacggcgac gcgacgaagg gcgaaaccaa caccgccacc gtcatgcccg
      601 tgaaggataa gatcctcgtc gggatctcgg gcggcgaatt cggggtccgc ggccacgtca
      661 ccgcctactc catggcggac ggcaaggtgc tctggcgcgg ctactccatg ggcccggact
      721 cggacacact catcgatccg gagaagacga cgcacctggg taagcccgtt ggcaaggaca
      781 gcggcctcac cacgtgggag ggcgaccagt ggaagatcgg cgggggcacc acctgggggt
      841 ggtatagcta cgacccggaa gaaaacctgg tgtactacgg caccggtaat ccgtccacgt
      901 ggaacccaac ccagcgcccc ggggataacc gctggtccat gacgatcttc gcgcgggacg
      961 tcgataccgg gatggctaaa tggctctacc agatgacccc gcatgatgaa tgggactatg
     1021 atggcgtcaa cgagatgatc ctcaccgagc agcagatcga tggtaaggat cgcaaactcc
     1081 tcacgcactt cgaccgcaac ggcttcggct acacgatgga ccgcgtgacc ggcgagctgc
     1141 tcgtcgccga gaagtacgat ccgaccgtga attgggcgac ggaggtcgtg atggacccga
     1201 aatcagacaa gtacgggcgc ccccaggtgg tcgcacagta ctcgacggag cagaacggcg
     1261 aggacaccaa caccaccggc gtctgcccgg ccgccctggg gaccaaggac cagcagccgg
     1321 ccgcgtattc gccaaagacg gaactgttct atgtccccac gaaccatgtt tgcatggact
     1381 atgagccctt ccgcgtgtcg tataccgccg gccagccgta cgtcggcgcc acgctctcca
     1441 tgtatccccc caaagactcg cacggcggaa tgggtaactt catagcctgg gacaacaagg
     1501 agggcaagat caagtggtcg ctgccggagc cgttctccgt ctggtcgggg gccctggcca
     1561 cggccggcga cgtcgtcttc tacggcacgc tcgaggggta tctcaaggcg gtcgacgcgg
     1621 ccacgggcaa ggagctctac cgcttcaaga cgcccagcgg ggtcatcggc aacgtgatga
     1681 cgtacgcccg ggaggggaag cagtacgtgg cggtgctgtc gggcgtcggc ggctgggcgg
     1741 ggattggcct ggccgccggc ctcacgaacc cgacggaagg attgggcgcc gtcggcggtt
     1801 actcggccct cagcaactat accgccctgg gcggcacgct cacggtgttc aagttgcccg
     1861 aggccgaaaa cctctatttt cagggacatc atcaccatca ccatcaccac tgacagct
//
