LOCUS       sequence-306549-        1918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018323
FEATURES             Location/Qualifiers
     source          1..1918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1866..1886
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1887..1910
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgcgcctgca tatgcgcggc gcgcggggca tgttgctcgt aggaggcatg ttgctccatc
      121 tcacgatgac cagctccgtg gccagcgcaa acgaggagct tatccgcctg gcgaaggacg
      181 agcgcaattg ggtgatgcag accaaggact atacagtgca gcgcttcagc aagctggacc
      241 agatcgacac gtcgaacgtg aagaacctcc agccggcgtg gtcgttctcc accggcgtcc
      301 tgcatggcca cgagggcggc ccgctcgtcg tcaatggaat catgtacgtg cactcggcgt
      361 atcccaataa cgtctacgcc atcgacctgg agcaccccga gaagattatg tgggagtatc
      421 gcccgaaaca gaaccccgcc gcccgcgcgg tcgcgtgctg cgacctggtc aaccgcggcc
      481 tcgcctacgg agacgggaag ctgatcctga gcacgctcga cggccagctc atcgcgatgg
      541 acgcgaagac gggaaaggtc atctggaacg tcgagacggc ggatgtcagc gtcgggcaaa
      601 cccagacgca ggcgcccttc gtcgtcaagg acaaagtcat catcggcagc gccggcgccg
      661 aactgggcgt ccgggggtac gtcacggctt ttaacctcaa agacggcaag aaggtctggc
      721 gggcgttcgc caccggcccc gataaggaca tcctcctgga taaggacttc aacaagcaga
      781 acccccatta tggccgcttc ggcctcgggc tcaagacgtg ggagggcgac gcgtggaaaa
      841 tcggcggcgg gacgaactgg ggctggtacg cgtacgatcc gaagctcgat cttatttact
      901 atggtagcgg caaccccgcc ccgtggaacg aaacgatgcg cccgggcgac aacaagtgga
      961 ccatgacgat gtggggacgg aaccccgaca cgggactggc gaagtatggc tatcagaaga
     1021 cgccgcatga cgagtgggac tacgcgggca tcaactacat gatgctcacg gaccaagtga
     1081 ttaatggcaa gatgacgccc ctcgtcacgc atccggaccg taacggcatc atctacaccc
     1141 tcaaccgaga gacgggcagc ctggtcgccg ccgataagat cgacccgtcg gtcaattgga
     1201 cccgtaaggt cgacctgaag accggcatcg ccatccggga ccccgagttc agcacccgca
     1261 tggaccacaa ggcgcgcggc atctgtccct ccgccatggg ctaccataac caggggctgg
     1321 acagctacga cccgcgctcg gaaatcttct tcatgggcgt gaaccatatc tgcatggact
     1381 gggagccgtt catgctcccg taccgcgcgg gtcagttctt cgttggggcg accctctcca
     1441 tgtatccggg cccgaagggc tacctgggcg aggttaaggc ttacgacgtc aaaaccaact
     1501 ctatgaagtg ggagaaggcc gagaagttct ccgtctgggg cggcaccatg gcgacggcgg
     1561 gcaacctcgt cttctacggc accctagacg gctggttcaa ggctctggac agtggcagcg
     1621 gcgaggagct atggaaattc aagctgccgt cgggcgtcat cggccatcca attacctacg
     1681 agcataatgg gaagcagtat gtcgcgatcc tctacggcgt cggcggctgg ccgggcgtcg
     1741 gcctggtgtt cgacctgaac gacccgacgg cgggcctggg cagcgtcggc gcgttcaagg
     1801 agctcgccaa atacacgaag atgggcggcg gccttatggt ctttgcgctg ccggagggcc
     1861 gcgccgaaaa cctctatttt cagggacatc atcaccatca ccatcaccac tgacagct
//
