LOCUS       sequence-306554-        1933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018333
FEATURES             Location/Qualifiers
     source          1..1933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1881..1901
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1902..1925
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacct
       61 tgggatggtt cggcctcgaa cgtatgcagg gccggaagcc gatgaacaag atcattatgg
      121 ccgccgtcgc cggcctcgtc gcgaccgcgg gctggcactc ggcggccctc gccgccggcg
      181 tcactgagga ggatctggcg aacgatacgg caaacacctc ctcagtgctc accaacggca
      241 tgggccggca tctgcagcgg tacagcccgc tcgatatcct caacaaggat aacgttaaga
      301 acctcatgcc ggcctgggcg ttctccctgg gcggggagaa gcagcgcggc caggagaccc
      361 agccgctgat ccatgacggc gtgatgtata tcacagggtc gtacagccgc atgtacgcta
      421 tcgatgtcaa caccggcgag gagctgtggc agtacgacgc ccgcctcccg gagggcatcc
      481 tcccgtgctg tgacgtcatc aaccgggggg gcgcgatcta cggcgacaag atctacttcg
      541 gaaccctcga cgccaggatc gtcgcgctcg acctcaagac gggggacgtc gtctggaaca
      601 aaaagatcgc cgactacaag gcgggctact cgtacacggc agccccgctc atcgtgaagg
      661 ggctcgtgat caccgggaac tcgggtggcg aattcggcat cgtcggcgaa gtccaggcgc
      721 gcgacgccga gaccggcgaa atggtttgga cccggcccgt gatcgaggga cacatgggga
      781 cgttcaaggg cgaggaatcg accatgaccg gcaccctgaa cgccacgtgg ccgggagaca
      841 tgtggaagac gggaggcggg gccacctggc tgggcgggtc gtacgacgcg gagacggaca
      901 ccctcgtctt cggcgccggg aacccggccc cgtggaactc gtggctccgg gacgcgggcc
      961 aaaacaacga tgggaagggc gacaatcttt atgcggcctc ccgcatcggc atcaaccccg
     1021 acaacggaga gatcaagtgg cacttccaga ccacgccgcg cgagggctgg gattacgacg
     1081 gcgtcaatga ggtggtcgcc tacactgaca gggacggcaa taagcgctgg gcgaccgcgg
     1141 atcgaaacgg ttacttctac gtgctcaatc gtgaggatgg caagttcgtg cgcggggtcc
     1201 cgttcgtcaa ggacatcact tgggcctcgg ggctggatga gaacgggcgc ccgatcttca
     1261 acgaggacaa ccgtcccggg gaccccgcag ccgcggcgga cggcgccaag ggcgaggtca
     1321 tcttcgcgag cccgtcgttc ctcggcggga agaactggat gccgatggcg ttctcgcaga
     1381 agaccggcaa cttctacgtc ccgtcgaacg agtggggtat ggatatctgg aacgaaccca
     1441 tcacctataa gaaaggcgcc gcctacctcg gcgccggctt cacgatcaag ccgaattacg
     1501 aggaccatat cggcagcctc aaggccatcg acccggacac aggcgagctg aagtgggaat
     1561 tcaagaacga tgcccccttg tggggcgggg tcatgaccac cgccggtggc ctggtgttca
     1621 ccgggacccc ggagggcaag ttcatcgctt tcgacgatga gaccggagag atcctctgga
     1681 gcttccagac gggctcgggc atcgtgggcc agccggtcac gtgggagatg gacggcgaac
     1741 agtacgtgag cgtcatctct ggctggggcg gggcggtgcc cctatgggga ggagaggtcg
     1801 ccaagaaggt gaactatctc aatcagggcg gcctcctctg gacgttcaag ctccccaagc
     1861 agctggcggc cgcgaacgcc gaaaacctct attttcaggg acatcatcac catcaccatc
     1921 accactgaca gct
//
