LOCUS       sequence-306557-        1936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018339
FEATURES             Location/Qualifiers
     source          1..1936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     RBS             764..772
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             1884..1904
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1905..1928
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgcccaattt ccgccgccac acgctgttcc tgagcctgat gcccctgctg gcgttccacc
      121 cgctgccgtc cttcgcccag accctcgccc aggagaacgt ctcgaagccc aaggcgcctg
      181 cgttcgctaa cgcgggcggt aacgagtggc ccatgcccgg cgcgaatacg gggctcacgc
      241 ggtatagctc cctgtctgac atcaacacag agaacgtggc caagctccag ccggcgttca
      301 ccttctccat gggtgtcgac cgcggcgagg agtcggctcc gctggtcgtc ggagatacgc
      361 tttacgccct gacgccctac cccaacatcc tctacgctct cgatctcagc aagccgggcg
      421 cccccctcaa gtggaagtac gagccgcatc cgctggcggc gagccagggc gtcgcctgct
      481 gcgacgtggt gaaccgggga gcgagcttcg cgaacggccg cgtattcttt aacaccctgg
      541 atgggaatac catagccgtc gacgcgaact ccggtgccga gatctggcgc acgcagatgg
      601 cgaacatcaa gatcggcgaa accatgacca tggcgccgct actggcgaag gacaaggtgc
      661 tggttggtga cagcggcggc gagatgggcg tccgcggctg gatcgccgcc ctcgaccagg
      721 gctcgggcaa ggtcctgtgg aaggcgttca gcacaggtcc agataaggag gtgctgatcg
      781 ggtcgggctt caacccgcac tacccgcagt tccaggggaa ggatctcggc gtgtcaacct
      841 ggccgtcggg catgtgggag cagggcggcg gcaccgtgtg gggctgggtc tcgtatgacc
      901 cggagctcaa cctgatctac tacggcacca gcaacccggg tccgtggaat tcgagcatgc
      961 gcccgggcga taacctgtgg accgccggca tcttcgcgcg cgacgccgat accggccagg
     1021 cccggtggtt ctaccagctc agcccccatg acgtctcgga ctacgacgcc gtcaacgagt
     1081 gcatcctcct tgacaccgag tggaatggca agaaccgtaa ggtgctcgtc catccggatc
     1141 gtaacggcta tgtctacatc atcgaccgtg aaaacggcga ggtgctggcg gccaatccgt
     1201 tcgtccacat caccagctct accaacgtcg atctaatgag cggccgcctc atctacaacc
     1261 aggccaagca gcctaaggtg gacgaggtga tgaccgacgt gtgcccggcc ccgccgggtg
     1321 ggaaggactg gcagccgtcc agcttctccg agagcaccgg cctgctgtac atcccgcaca
     1381 acaacctctg catggatatc gaggaaaagg acgtcggcta catcgcggcc accccgtacg
     1441 tcggcgccaa cgtgcatatg aacgccgggc caggcggcaa ccgcggcgtc atgtccgcgt
     1501 gggatatcca agcggggaag gaggtttgga agatcgatga atccttcccc gtttggtcgg
     1561 gggccctggc caccgccgga gacgttgtct tctatggcac aatggagggc tggttcaagg
     1621 cggtcgatgc gaagaccgga aagctcctct ggcgcttcaa gacaggctcg ggcatcatcg
     1681 gccaaccgac gacctggcgc ggtcccgacg gcaagcagta cgtctcggtg ctgtcgggag
     1741 tgggcgggtg gccgggtgcc atcgtggccg gaggtctcga caagcgggac ggtaccgccg
     1801 cgctcgggtt cgtcaacgcg gtgaaggacc tgccggagca taccaccaag ggcggcacac
     1861 tttacgtgtt cgccatcccg gccgaaaacc tctattttca gggacatcat caccatcacc
     1921 atcaccactg acagct
//
