LOCUS       sequence-306558-        1939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018341
FEATURES             Location/Qualifiers
     source          1..1939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1887..1907
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1908..1931
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgtcgctgaa gcgcgccact ggccagaccg gcctccagaa gaagcgcctt tcgcgcgcgg
      121 tggcgaccgc catcgccggg tttgccctgg cctcagcgtc gggcgctgcc ctcgccaacg
      181 ccgagctgat caagctgtcg caggacgatc gccaatgggt catgcagggc aaggactatg
      241 accactcgca ctacagcggt ctgaagcaga tcaatcggtc gaacgtcaag aacctcaagg
      301 cggcctggac cttctccacc ggcgtcctcc acggccatga gggcggcccg ttggtggtga
      361 acggcatcat gtacatccat acgcccttcc cgaacatcac gtacgccatc gatctcgata
      421 accccaacaa gatcctctgg gagcacaagc caaagcagga tgccagcgtc cgggccgtgg
      481 cctgctgcga cgtggttaac cgcggcctgg cgtacggcga cggtaagatc ttcaagaccc
      541 agctcgacgg ccacatcgtc gctctcgatg ctaagacggg gaaggagctc tggaagatgg
      601 agaactcgga ccccaaggtg ggcagcacgc tgactcaggc gccgatggtt gtcgaggaca
      661 tggtgatcgt ggggtccagt ggcgcggagc tcggcgtccg cgggtatgtc accgcgtacc
      721 gaatcaagga cggcaagcag gtgtggaggg cctacgccac ggggcccgac gaagacatac
      781 tgctggcgga cgatttcaac atggccaacc ctcactacgg acagaagggc ctgggcacat
      841 ctacgtggga gggggacgcc tggaagatcg gcggcggcac caattggggc tggtatgcct
      901 acgatccgga gcttaagctg atgtactatg gcagcggcaa ccccgccccc tggaacgaga
      961 ccatgcggcc gggcgacaac aagtggacca tggctatctg gggccgtgac gtcaagacgg
     1021 gcaaggcgaa gttcgccttc cagaagacgc cccacgacga gtgggattac gccggggtca
     1081 attggatcgg actgtccgac caggtcgtcg atggcaagaa gcagaagctc ctgacccatc
     1141 cagaccgcaa tggcatcgtg tacaccctga atcgcgaaaa cggcaacctc gtgcgcgccg
     1201 acaaaatcga tccgtcggtg aacgtgttta agggctacga tatgaagaag ggcgtcccga
     1261 tccgcgaccc tgagtattcc acccgtatgg atcacctcgc caagggcatc tgcccctcag
     1321 ccatggggta ccacaaccag ggacacgatt ctatcgacaa gaagcgcgag ctggtcatgc
     1381 tcgggacaaa catgatctgc atggactggg agcccttcat gctcccctac cgggcggggc
     1441 agttctttgt gggcgcgacg ctgaatatgt acgcgacccc gggcaacaac ggtaacatgg
     1501 ggcaggtgaa ggcctatgac gtgaagacga gcaagttcaa gtgggtcaag gacgaaaagt
     1561 tctccgtgtg gggcggcacc acctccaccg ctggcgacct ggtcttctac ggcaccctcg
     1621 acgggctgat caaggccgtg gacgccgaca atgggaagac cttgtgggag ttcaagctcc
     1681 cctcgggcgt catcggccat cccgtcacat acgagcacaa aggcaagcag tacgtcgcga
     1741 tctattacgg tgtcgggggc tggccgggcg tcggcctggt cttcgacctc accgatccca
     1801 ccgcgggtct gggcgccgtg ggggcgttca aggagctgca gcagtacacg aagatgggcg
     1861 gtggcgtgat ggtgttctcc ctcgccgaaa acctctattt tcagggacat catcaccatc
     1921 accatcacca ctgacagct
//
