LOCUS       sequence-306565-        1966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018355
FEATURES             Location/Qualifiers
     source          1..1966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1914..1934
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1935..1958
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgaagaagcc ggtgaagaac tggctactgg cctcgaccgt ggccaccctc ctcgccgccc
      121 cgaccgtgtc cacggccaac tcggacgtgg aaaaactgac ccaggacccc gccaactggg
      181 ccacctgggg cggagactac gccggcaccc gctactccaa gctcagccag atcaacgccc
      241 agaacgtcaa gaatctgcag ccggcgtggt cgttcagcac aggggtcctc cgggggcacg
      301 agggcggccc cctcgtggtc aacggagtca tgttcatcca taccccgttc cccaacaccg
      361 tctacgcgat cgaccagaag acgaaggcag tcatctggga gtttacgccg accatggacg
      421 ccgacgttac catcccggtc atgtgctgcg acaccgtcaa ccgcggtctg gcctacggtg
      481 acgggaagat tttccttcag cagtccgaca cggtgcttac agccctcgac gccaagacgg
      541 gcaaacgcgt gtggtcggtg cagaatggcg accccaagct ggggatgaca aataccaatg
      601 cccccctggt cgtcaaggat aaggtgatca cgggcatctc gggcggcgag ttcggcgtgc
      661 gcggcttcct ggccgcctac aacatccgga ccggccagct ggaatggaag gggtactcga
      721 tgggccccga caaggacacg ctgctgaagc cgggtaagag caccacctgg caggatggta
      781 aggtgacgcc ggtgggcgcc gattccggca cgtccacgtg gaagggcgac cagtggaaga
      841 tcggtggcgg gacgacctgg ggctggtaca gctacgatcc gaagctcaac ctcgtttact
      901 acggctccgg taacccgagc acctggaacc cggtccagcg gccgggcgac aacaagtggt
      961 cgatgtctct ctgggcccgg gacgccgaca ccggagaggt gaagtgggtg tatcagatga
     1021 caccgcacga tgaatgggac tacgatggca tcaacgagac cgtcctggtt gaccaggagg
     1081 tgaagggcaa gatgcataag accatcgtgc acttcgacag gaacggcttc ggctacacgc
     1141 tcgaccggga gacgggggaa cttctcgtcg ccgaaaagtt cgacaagtcg gtcaactggg
     1201 cctcccacgt cgatctgaag tcgggccggc cggaggtcgt gccggaattc tccacggagc
     1261 acaacggcga ggatgtgaac acggtcggca cgtgccctgc ggccctcggc tcgaagaacc
     1321 agcagcccgt ctcctactcg cctcagaccg gcctgttcta tatctcgggg aaccacctct
     1381 gcatggagta cgagccgttc gaggtctcct acaccgccgg gcaaccgtac gtgggcgcca
     1441 ccctgtccat gatgcccgcg ggcgcggacg tcctcaccgg caagaaggac ggcaccacca
     1501 acctcggcca gttcacggcg tacgatgcga agacgggcaa gatcgcttgg agcaacaagg
     1561 agcaattctc ggtctggtcg ggctcggtgg cgactgcggg cggcgtcgtc ttttacggca
     1621 ccctggaagg ctacctgaag gccgtcgacg cgaaaaccgg caaggagctc tacaagttca
     1681 aaaccccgtc gggcatcatc gggaacgtga acacctggga attcgagggc aagcaatacg
     1741 tgggcgtcct ctcgggcatc ggcggctggg ctggcatcgg gatcgcggcc ggcatcgacg
     1801 acgggacctc cgcgtcgtcc tccgaggggc tgggcgcggt gggcgcctac cgcagcctct
     1861 cgagctacac caagctggga ggcacgctga ccgtgttcgc gctcccgaac gccgaaaacc
     1921 tctattttca gggacatcat caccatcacc atcaccactg acagct
//
