LOCUS       sequence-306568-        1972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018361
FEATURES             Location/Qualifiers
     source          1..1972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1920..1940
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1941..1964
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgcgcaaggt cctcctctcc gcgacggccc ttgccaccgc catcgctagc acgctcgcgc
      121 tcgcgaacga caagctcgtc cagctctccc agtcggaaga aaactgggtc atgacgggca
      181 agaactacaa cgcgaacaac tattcgaagg cgacccagat caacgagaag aacgtcaagc
      241 agctccggcc cgcctggacc ttcagcacgg gcgtcttgaa cggccacgaa ggcacgccgc
      301 tggtcgtcaa cgggacgatg tacatccact ccccattccc gaacctcacc ttcgccctgg
      361 acctcgataa cccgacccgc atcaagtgga tggataagcc caaacagaat gccgccgcgc
      421 gcgcggtggc ctgttgcgat gtcgtcaatc gcgggctcgc ctattggccg ggagacggca
      481 aggtgccgcc gctgatcttt aagactcagc tggatgggca catcgtggcc ctcgacgcga
      541 cgaccggcgc ggtgcactgg aagcttgaaa actcggacat ccgtgtcggc tcgacgctca
      601 ccatcgcgcc gtatgtcgtc aaggattacg tcatcgtcgg cagcagcggt gccgagctgg
      661 gcgtgcgcgg ctacctgacc gcctacgacg tcaagacggg cgagcagaag tggcgcgcct
      721 acgccaccgg cccggatgac gagctccgcc tcgccaagga cttcaactcg gcgaacccgc
      781 actacggcca gttcggtctg ggcctcaaga cctgggaggg cgacgcctgg aagatcggcg
      841 ggggaaccaa ctgggggtgg tacgcgttcg accccggcac caacctgatc tacttcggca
      901 gcggtaaccc ggcgccgtgg aacgagacga tgcgcccggg cgacaacaag tggacgatga
      961 ccatctgggg ccgcaacgtc gacaacggcg aggccaagtt cggctatcag aagaccccgc
     1021 atgatgagtg ggactacgcc ggcgtgaacg tcatgatgct gtccgagcag accgacaagg
     1081 ccggcaagaa gcgcaagctg ctgacgcacc cggaccgtaa cggcatcatc tacacgctcg
     1141 accgggagaa cggggacctc gtgtcggccg ataagctgga cgacaccgtc aactgggtga
     1201 agtcggtgga cctgaagacg ggcctccctc tgcgcgaccc ggagtttgcc actcgcatgg
     1261 accacaacgg tcgggatatc tgcccgagcg cgatgggcta ccataatcag ggccacgact
     1321 cctacaaccc ggagaagcag acgttcttcc tcggcatcaa ccacatctgc atggattggg
     1381 agcccttcat gctcccctac cgggccggcc agttcttcgt cggcgcgacc ctgaacatgt
     1441 acccgggccc gaagggcgac cgccagaact acgagggact cggccagatc aaggcttaca
     1501 atgctatcac gggcgaatac aagtgggata agatggagcg cttcgcggtg tggggcggca
     1561 ccctcgccac cgcaggcaac ctggtgttct atggtaccct ggatggattc attaaggcac
     1621 gccatgccga caccggcgac ctgctctgga agttcaagct gccctcgggc gtcatcggcc
     1681 acccgatgac gtacacgcac aaggggacgc aatacgtcgc catcttctac ggcgtgggtg
     1741 ggtggcccgg catcggactg gtccaggacc tggaggaccc cacggcgggc ctcggcgccg
     1801 taggcgcctt caagaagctg gccaactaca cccagatggg cgggggaatc atggtcttct
     1861 ccctcgatgg caagggcccc tacgacgacc tttccctggg cgagtacgcc agcaacgccg
     1921 aaaacctcta ttttcaggga catcatcacc atcaccatca ccactgacag ct
//
