LOCUS       sequence-306569-        1978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018363
FEATURES             Location/Qualifiers
     source          1..1978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1926..1946
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1947..1970
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgccccgccg tgagcgcacg cgctgcgtca gcgaccgccg ccgcttgttc gtggtcgcag
      121 tgggcggatc gctcttcctg gccggctgca agggcgacaa ggagaaggac gacccgcgcg
      181 tggccctggc cgcgcgggtc ggtcccgcca cgaccagcgg cgacaccacc acgaagttcg
      241 cggatgacgg ccagtgggtg cggccggcga aggacttcca gggtacccgc ttcagcgggc
      301 tcaccgagat caacaacacg aacgttcaga agcttcacgt cttcggcacc ttcagcttgg
      361 ggacgacccg cggcgtggag gcggcgccca tcgtggccgg cacgacgatg tatatcgtca
      421 cgccgttccc caacttcgtc tacgccctgg acctcacgaa ggagggtatg ccgaccaagt
      481 ggagctacaa gccgcgcccg gtcaacgccg cccagggcgt ggcgtgctgc gacgtggtca
      541 accgcggtct cgtagtcgat gccggaaagg tgatcttcaa taccctggac ggccgcacca
      601 ttgccctgga cgcagccacg ggcaaggagc tctgggagac ccagctggcg aacatcaacc
      661 tgggcgagtc cgtgacgatg gcccccctcg tggtgaaggg caaggtgttc gtgggggtgt
      721 ccggcggcga gttcggcgtg cgcgggtggc tgaaagcggt ggacgagaac tccggcaagc
      781 tcgcgtggac ggcctaccac accggccccg acaaagacgt cctcatcggg cccaacttca
      841 agcccttcta taagatggac cagggcaagg atctcggcgt gtccagctgg ccggccgacc
      901 agtggaagat cggcggcgcc ggcgtttggg gctggatcac ctacgatccc gagctggacc
      961 tcatctacta cgggacggcc aaccccggac cctggaaccc cgagatgcgt cccggcgata
     1021 ataagtggag ctccacggtg ttcgcgcgcc atccggataa cggggaagcc gtctgggcgt
     1081 accagctgtc ccctcacgac gagcacgact atgatggcgt caacgagaac atcctcgtgg
     1141 acatggactg gaagggtacg cgccggcagc tcatccttcg cccggaacgc aacggctact
     1201 tctacgtgtt cgaccggaaa accggggaag tcctctccgc ggaacccttc ggcttcgtca
     1261 acacgaccac gggcgtggac ctgaagacgg gccgcctgca atatgcgccc gagaaggcga
     1321 cgcgcatcgg ccaggtggtg cgctactcct gcccggcagc cccgggcatg aaggattggc
     1381 agccgagcgc gtactccccg cgcacgggcc tggtgtacat cccccatcag aacctttgca
     1441 tggacgaaga ggggctcgag gcgaactata tcgccggcac gccctatgtg gggaccaacg
     1501 tcaagatgta cgcgggcccc ggcgggaacc gtgggttctt cgatgcctgg gacccggtcg
     1561 gccagcacaa ggtctggagc atcaaggaag atctgccggt gtggagtggg gcgctcgtga
     1621 cggcaggcga tgtggccttt tacggcacga tggatggctg gttcaaggcg gtcgacgcgc
     1681 gcaccggcaa cccgctctgg cagttcaaga cggggtccgg catcatctcg cagccgatga
     1741 cctactcgta tgggggtaag cagtacatcg ccgtcctcag cggcgtcggc ggctggtcgg
     1801 gcgccatcgt cgccggcggc ctggacgccc gggacagctc ggccgccctg ggtttcgtga
     1861 acgccatgaa ggacctgccg cagaagtcga ccaagggtgg catgctgtac gtgttcgccc
     1921 tggccgaaaa cctctatttt cagggacatc atcaccatca ccatcaccac tgacagct
//
