LOCUS       sequence-306571-        1984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018367
FEATURES             Location/Qualifiers
     source          1..1984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1932..1952
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1953..1976
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgaagcgcag ggcgtaccac cggctgctct tccttctcat cctctcagcc gtctcgtggg
      121 tcggcctgtc gatcgccaac ccccaggtcg aagaactgtc gaagaaggcc ggcctctggc
      181 ccgcccccgg ccgggactac cacctcacgc gctacagccc gctgagccag atcaatacgc
      241 ataacgtgaa gaacctcgac ctggcctgga gcttctcgac cggcgtcctc cgcggccacg
      301 aaggccagcc gctcgtgatc ggcaacacga tgtatgtgca gaccgcctat cccaacatcg
      361 tctatgccct ggatatctcc aagggaaacg actacaagat cctgtggaag tacgtcccgc
      421 aacaggacga ccgcgcggtc gctgtggcct gctgcgatac cgtcatgcgg ggaatctctt
      481 acgccgacgg caagatagtc atgaacacgc tggatggcca cgtcatcgca ctcgacgcga
      541 agacaggcaa ggagctgtgg aaggtcaagc acgccgaccc gtacaagggc gaaaccatca
      601 cgatggcgcc catcgtggcc aaagacaagg tcatcgtcgg catctcgggc ggcgagttcg
      661 gcgtccgggg gcgtgtcgcg gcgtacgatc ttaactccgg ccgccgcgtc tggctgtgct
      721 acaaccaggg cccggaccag gacatgtgct acggctcgcg caccccggcc agctacaagg
      781 gactgggcgt caacacctgg cagggcgatc agtggaagat cggcggggcc accgtgtggg
      841 gctggttctc ctacgacccc aagctcaacc tggtctacta cggcacgtcg aacccgggca
      901 cgtggaaccc ctatatccgg tgttccgcct cctgggagga atgccagtcg ggccgctacg
      961 acaacaagtg gagcacctcg atcatcgccc gcgatgccga caccggcgag ttggtctggg
     1021 cgttccagat gaccccgttc gaccagtggg attacgacgg cgtcaacgag tccatcctgc
     1081 tcgatgtgaa cgtcggcagt aagaaggtgc cagcgctgat tcacttcgac cgcaacggct
     1141 tcgccttcct cctcaaccgc gccacgggcg agctgatcta cgctaagccg ttcgtgtacg
     1201 tcaactgggc gaagtcgtgg gatgagcggc acgcgcgcga cggtcacact ttcaaggtca
     1261 cgaaggaccc cacgaaaagc ccgtggaaac cgggcgacaa caagaaggac gtctgcccct
     1321 ccgcgatggg cggcaaggac cagcagccgg cggcgtacag cccggaaacc ggcctggtct
     1381 atgtgccgac caaccagtgg tgcatggact acgagccagc cgagaccaag tacaccgagg
     1441 gcgcccccta cgtcggcgcg aatgtgctta tgaagtttta cgagcacaag tcgtcggtcc
     1501 acggcctgtc caagaccccg ggccactacg gtcgcatcat agcctgggat ccggcgaagg
     1561 gcgagatcaa gtgttacatc gacgagaagt acccgacctg gagcggcgtc cttgttacca
     1621 agggcggcgt cggcttctac ggcacgatgg acggttggtt ccgcgcgttc gacctgaaga
     1681 cctgcaaggt cctctggtcg aagaagctcg gatcgggcat catcggaaat ccgatctcct
     1741 tcgaggtcaa cggcaagcag tacatcgcgg tgtactccgg aatcgggggc tgggcgggcc
     1801 tgcccgtcac cgccaacctg gacccgaacg acccgtatgg cggcctgggc gccacgaacc
     1861 tctccctcga gctggatctc taccggcagg ccacgatgtc cggcgtcctg tacgtgtttt
     1921 cgctggaggc cgaaaacctc tattttcagg gacatcatca ccatcaccat caccactgac
     1981 agct
//
