LOCUS       sequence-306572-        1990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018369
FEATURES             Location/Qualifiers
     source          1..1990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1938..1958
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1959..1982
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgaaggcgat gccgcagctc gcgctgaccc gcgtcgacgt ggagcgccgt acacgcaagc
      121 tggccgccgg aaacagcttc ggcgcgatcc tcctggtcac cctcctcgcg tgcttgctgc
      181 tcttgggcgc cgccagcgat ccgccgaaga cgccggcgat cgtgccgccg gccgcggcgt
      241 ccccgccgga cgatggccag tggaccatgc ccgccaagaa ctatgcctcc acccgcttct
      301 cggagctctc cgagatcaac gagggaaacg tgaagaacct ccaggtcgcg ttcaccttct
      361 ccacgggcgt gaacaagggc caggaggccg cgcctctcgt ggtcggcaac acgatgtaca
      421 tcgtcacccc gttcccgaac ttcgtctacg ccctcgatct ctcgaagccc ggtgccccca
      481 tgaagtggaa gtacgagccc aaccccgaac ccgccgcgca gggcgtggcg tgctgcgacg
      541 tggtcaaccg cggcgttgcc ttcgccgatg gccgcatctt cttcaacacg ctggacggcc
      601 acaccatcgc cctggacgcg aatacgggca agcccatctg gaacacgcac gtgggcaaca
      661 tcaacatcgg cgagaccatc accatggcgc cgctggttgt caaggggaag gtgctcgtcg
      721 gcaactccgg cggcgagatg ggggtccgcg gctgggtgaa ggcgctggac gctggcgatg
      781 gccatgtagt gtggatcgcg ttctcgaccg ggcccgacaa ggacgtgctc atcggccccg
      841 atttcaagcc gcattacgac atggacaagg gcaaggacct cggggtgacc acctggccgc
      901 ctgaagcctg gaagatcggc ggcggtaaca tgtggggctg gatctcctac gatcccgatc
      961 tcaacctgat cttccacggc accggcaacc ccggcccctg gaaccccgac ctgcgcccgg
     1021 gcgacaacaa gtggacctcc ggcatcttcg cgcgggaccc agataccggt gccgcgaagt
     1081 ggttctacca gtggagcccg cacgacctcc acgactacga cggcatcaac gagcagatcc
     1141 tcctggacat gacctggcag ggcaagccgc ggaaggtcct gatccggccg gaacgcaacg
     1201 gctacctgta catcctggac cgcaccaccg gcgaggtgct gtcggccaag ccgttcggcc
     1261 ctgtcaactc ctcgaagggg gtggatctga agaccggccg cctcatcgtg aacccggaca
     1321 agaagacggg gaccggcaag gtggtccggg acatctgccc cacggcgtcg ggactgaagg
     1381 actggcagcc gtcggccttc tcgccgaaga ccggcctgct gtacatcccg cacaacaacc
     1441 tgtgcatgga cgaggagggt gtcgaggtca actatatcgc tggcaccccg tacgtcggaa
     1501 tgaacgtccg catgatcccg ggccccggcg gcaaccgcgg ggcgttcacc gcctgggaca
     1561 tcgcagcgga gaagccggcc tggagcctca aggaaaattt tccagtgtgg tcgggagcag
     1621 tggtgaccgc gggcgatgtc gtgttctacg gcaccatgga aggctggttc aaggcggtct
     1681 cggcgaagac gggcgacctg ctctggcagt ttaagaccag cagcggcatc atcgggcagc
     1741 ccatcactta ccgcggcccg gatgggcacc agtatgtcgc gatcctgtcg ggcgttggcg
     1801 gctgggcggg cgcgatcgtg agcggagacc tcgacccccg cgacgccacg gccgcgctgg
     1861 gcttcgtgaa cgcgatgaag gacttgaaga atgcgaccac ctcgggcggc accttgtacg
     1921 ttttccggct ccccgccgaa aacctctatt ttcagggaca tcatcaccat caccatcacc
     1981 actgacagct
//
