LOCUS       sequence-306573-        1993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018371
FEATURES             Location/Qualifiers
     source          1..1993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1941..1961
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1962..1985
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgaaggggat gctccacccg gcccgcgcac gggtcgacgc gggccagggc gcccgcgccc
      121 tcgccacaga gaacagcttc cgcacccgca tacttccggt gatgctgctc gcctgcggcc
      181 tcttcctgtc cggcgcgagc gaaccggcca gcgctgcggc catcgccccc ccggccgcgg
      241 ccgccccgcc cgatgacggc cagtggacga tgcccgcgaa gaattatgcc tccacccgct
      301 tctcggagct gtcggaaatc aacgagggca atgtcaaaaa cctgcaggtg gccttcacct
      361 tctccaccgg cgtcaacaag ggtcaggagg ccgcaccctt ggtcgtcggc aacaatatgt
      421 acatcgtcac ccccttcccg aacatcgtct acgccctcga cctctcgcgc ccgggcgcgc
      481 ccatgaagtg gaagtacgaa ccgaaccccg agcctgccgc gcagggtgtg gcgtgctgcg
      541 acgtggtgaa tcgaggcgcc gccttcgcgg acggccgtat ctttttcaac accctggatg
      601 gccacaccat cgcgctcgat gccaacaccg ggaagccgat ctggaacacc cacatcggga
      661 acattaacat cggggagacc atcaccatgg cgccgctggt ggtcaagggc aaggtcctcg
      721 ttggcaacag cggcggcgag atgggggtcc gcggttgggt caaagcgctc gatgccggag
      781 acggtcacgt cgtctggacc gcttacggaa ccggccccga caaggacgtc ctcatcggcc
      841 ccgacttcaa gccgcactac gatatggacc ggggcaccga cctgggcgtc acgacctggc
      901 cgccggaggc ctggaagatc ggcggcggga atatgtgggg ctggatcagc tacgacccgg
      961 atctgaacct catcttccac ggtacgggca acccgggccc gtggaacccg gacctccgcc
     1021 cgggcgacaa taagtggacc tcgggcatct tcgcgcgcga cgccgacacc ggcgcggcta
     1081 agtggttcta ccaatggtct ccccacgacc tgcacgacta cgacggcatc aacgagcaaa
     1141 tcctgctcga tatgacgtgg cagggtaagc cgcggaaggt gctggtgcgc cctgaacgga
     1201 acggctacct ctacatcctg gaccgcacca cgggggaggt cctctcggcg acggcttacg
     1261 gtccggtgaa ctcgtcgaag ggcgtcgact tgaagacggg tcgcctgatc gcgaatcccg
     1321 acaagcgcac aggtaccggc aaagtagtgc gagacatctg ccctaccgcc tcgggcctga
     1381 aggactggca gccgagcgcg ttctccccac gcaccggcct gctctacatc ccccataaca
     1441 acctctgcat ggacgaggag ggcgtggagg tcaactacat cgccggtacc ccgtacgtcg
     1501 gcatgaacgt gcggatgatc ccgggcccgg gcggcaaccg tggcgcgttc acggcgtggg
     1561 acgtggcggc ggcgaagccc gcgtgggtcc tgaaggagaa cttcccggta tggagcggca
     1621 ccgtggtgac cgcgggcaac gtggtgttct acggtacgat ggaaggttgg ttcaaagccg
     1681 tcagcgcgcg caccggcgac ctcctctggc aattcaagac cagctcgggc atcatcggac
     1741 aaccgatcac ctaccgtggc ccggacggcc atcagtacgt cgcgatcctg agcggcgtcg
     1801 gcgggtgggc gggcgctatc gtctccggtg acctcgaccc ccgagatgcc acggccgccc
     1861 tgggcttcgt gaacgccatg aaggacctga agaacgcaac cacggccggc ggcaccctgt
     1921 acgtgttccg cttgccggcc gaaaacctct attttcaggg acatcatcac catcaccatc
     1981 accactgaca gct
//
