LOCUS       sequence-306574-        1999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018373
FEATURES             Location/Qualifiers
     source          1..1999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1947..1967
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1968..1991
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgcgggagaa gaagctctcg cgcctgacga agaccgggtc ggcggccctc gtctcctccg
      121 taatcgggat gatgaccgtc ggggccgcgt tcgccaacga caagctcgtc gaactccaga
      181 aggacgaggc gaactgggtc atgcagggca agaactacag cgcggaccac tactcgtcct
      241 cgaagcagat caacaagaag aatgtgtcga agcttgcacc gtcgtggacc ttttcgacgg
      301 gggtcctgtc gggccacgag ggcgccccac tcgtgatagg ggatatgatg tacatccaca
      361 gcccgttccc caacaacacc ttcgcgatca acctgaacga tccgggcacg atcgtgtggg
      421 agcataagcc caagcaggac cccgccgcgc gcgcggtcgc ctgttgcgac gtcgtcaacc
      481 gcggcctggc ctactggccg ggcgatgata agacgccggc catgatcgtc aagagccagc
      541 tggacgggca cgtcgtcgcg ctcaacgccg agaccgggga actccactgg caggtggaga
      601 atggggacat ctcggtcggc cagacgcaga cccaggcccc cttcgtggtc aaggacctcg
      661 tcatccaagg ctcctcgggc gcggagctgg gtgtgcgggg ctacgtcacc gcgttcgaca
      721 tccataccgg cgagatggcc tggcgctggt atgccacggg tcccgattcg gaaatcgggc
      781 tcgccaagga cttcaacagc gaaaacccgc actacggcca gaaggacctc ggcctcaaga
      841 cctgggagga cgaggcctgg aagatcggcg ggggcaccaa ctggggctgg tatgcgtacg
      901 acccggacct cgagatgttc tactacggca gcggcaaccc ggccccctgg aacgagacca
      961 tgcgcccggg cgacaacaag tggaccatga ccatctgggg gcgcgacgcc gatacgggcc
     1021 ttgccaagtt cggctaccaa aagacgcccc acgacgagtg ggattacgcg ggcgtgaacg
     1081 tcatgatgct gagcgaacag aaggacaaga gcgggaagat gcgtaagctg ctgacgcacc
     1141 cggatcgcaa tggcatcatc tacacgctcg accgcgagac gggcgacctg gtctccgcgg
     1201 acaagatgga cgataccgcc aactgggtga agaaagtgga cctggagacc ggcctgccga
     1261 tccgcgaccc ggagttctcg acgcgcatgg ggcaccggtc gcgtgacatc tgcccgtcgg
     1321 cgatgggctt ccacaaccag ggcatggaca gctacgaccc cgagcgccag aatttctacc
     1381 tcgggatcaa tcacctctgc atggactggg agcccttcat gctgccgtac cgtgcgggac
     1441 agttcttcgt gggcgccaac gtgtggtcgt acccgggccc caagggcgac cggcagcgcg
     1501 ggatcgggtc gggccaggtc aaagcctata acgccatcac cggcgagttc gcgtgggaaa
     1561 agatggagaa gttctcaatc tggggcggca cgatggccac ggccggcggc ctcgtgttct
     1621 acggcacctt ggacggtttt atcaaagcgc gggacgcgga caacggggac ctgctctgga
     1681 agttcaagct gccatccggc gcgatcgggc atccgatgac ctacaccaag gacggcgtac
     1741 agtacgtcgc catcaactac ggggttggcg gctggccggc cgtgggcctc gtgttcgatc
     1801 tcaacgaccc ctccgcgggc ttgggtgcgg tgggcgcctt caaggagctg gcccgtaaca
     1861 cgcagatggg cggtggcttg atggtcttcg ccctggacgg caagggcccg tacgatgacc
     1921 tcagcgtggg agagtatgaa atggccgaaa acctctattt tcagggacat catcaccatc
     1981 accatcacca ctgacagct
//
