LOCUS       sequence-306577-        2017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018379
FEATURES             Location/Qualifiers
     source          1..2017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             complement(1116..1127)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1965..1985
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1986..2009
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgggcaagcc gctcagcggc agcaccatgg ccggcatgat cgctggcgcg ctgatgctgg
      121 ccatcgcctc cgggtccgcc ctcgccaacg aggagatcgt gcgagcatcg aagaatcccg
      181 acctgtggcc cgccccgggc cgcgacctgg ccatgacgcg gcactccccg ctgaaggata
      241 tcaacacggc caatgtaggc aagttgcaga tgatgtggag ccagtcgacc ggcgccctcc
      301 gcggccacca gggacaaccg atcgtcgtgg aggtggccgg cacgccgatg atgttcttcg
      361 tgagcccgtg gccgaacatc gtccaggccc tcgacctgag cgacccggac cacccgaagc
      421 aggtctggaa ctataagaag aaaaccgacc gcgacgagct ggccgtcacg cgcgcctgct
      481 gcgacaccat caaccgcgga ctgaactacg cggacggcaa ggtggtcttc cacaccctgg
      541 acggctacgt catcgcgctc gatgccaaga cgggcaaaga gatctggacg gtcaagcacg
      601 cctggtctga caagggcgag acgatcaccg gcccgacgct gatcgccgag aacaaggtga
      661 tcattggctt cggcggcgac gagttcgccg cgcggggccg cgtgacggcg tacgacctca
      721 agaccggcaa gcaggtctgg aactgtcact cgaccggctc cgacaaggac gtatgcctta
      781 cgcccgagac caacaaagcc aacccgcact acggtacgta cgggaagaat ctcgggatga
      841 tcacgtaccc gaacgacgaa tggaaaatcg gcggcggcgc gccctgggag tggttcacct
      901 acgacccgga gcttcggctt gtgtacgtgc ccaccggcaa cccggggcat tggtcgccgt
      961 cctaccgctg cggcgagacc ggggccaatc tgacccacga gaaatgcaac cagtacgatc
     1021 ccgagctccg gtccggcagg tgggacaaca agtgggcgat gacgatcttc gcgcggaaga
     1081 tcgataccgg cgaagccgtg tgggcgtacc aaatgacgac cttcgatcag tgggattacg
     1141 atggggtcaa tgagccggtg ctcgtggaca tgaaggtgga tggcaagatg cggaagaccc
     1201 tcgtgcactt tgaccgcaac ggcttcgcct acgtcctgga ccgcgccgac ggcaccctcc
     1261 tgcgcgccca caagttcttt ccgctgaact gggcggagaa agtcgacctc aagaccggcc
     1321 gcccggtcaa gatcaaggag cattccccat tcgcccgcca tgtttcgacc caggtctgtc
     1381 cgtcgagcct tggggcgaag aaccagcagc ctattgccat cgaccccaag gagccccaca
     1441 tcgcgtacgt ggcgacgaac tggtggtgca tggagtacga gccgcaggag cggacccata
     1501 cccagcaggg aatgctctac gtgttcgcga atgtgttcac ctacccgatc gagaagggcg
     1561 tggcgtcgaa ggtgcagaag ttcaacgtcc tcactggcga aaccgagtgg gcgatcccgg
     1621 acgcctatcc ggactggggg ggcatgctga ccaccgacgg cgacctcctg ttctatggct
     1681 ccctcagcgg ggatttccgc gccgtcgatc gcaagtcggg caaagtcctg tggtcccgca
     1741 agctgggcag cgggatcatc ggaaacccga tcacctacaa gatcaagggc aagcagtacg
     1801 tctccgtcct ggccgggatc ggcggctgga tcggcctgcc ggtgaccgcc gaattggact
     1861 tcgacgacaa gtacggcgcc atcggggcga ccgcgatggc gaagctgacc ggtctcgaca
     1921 agatcccgca aggcggcgtg ctttacacct tccgtatcga cgccgaaaac ctctattttc
     1981 agggacatca tcaccatcac catcaccact gacagct
//
