LOCUS       sequence-306580-        2029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018385
FEATURES             Location/Qualifiers
     source          1..2029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1977..1997
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1998..2021
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgaagctcag cctcggcgtt ctgacgggca cgatcgtcgc ggtcacgatg ggcctcgcgg
      121 gtcaggcctc cgccatgaac accgacgagg agcagctcaa gcgcatggcc gacccggacc
      181 aatggcccgc cccgggccgc gacttcgccc tgacccgcca tagctcgctg tccgacatct
      241 cgaccgaaaa cgtcaacaaa ctgcagatgg cgtggatgca gggcacgaac gcgctgcgtg
      301 gccatgaggg ccagccgctg gtcatcaagg acgtgggcgg caagcccatg ctcttcatga
      361 tcagcggctg cccctcgatg gccaactgca atgtggtcca gggcctggac ttgtcggatc
      421 cggacaaccc gaaacaggtc tggagctatg ttaagaagac cgaccgcgac gagtcggctg
      481 tgccgcgggc ctgctgcgat accgtcaacc gcggcggcag ctacgcggat ggcaagttcg
      541 tcttcggcac cctggacggg ttcctgatcg cactggacgg ccagaccggc aaggaagtgt
      601 gggtggtgaa gtgggcgtac cccgagaagg gcgagaccat caccccggcc ccgctgatcg
      661 cggacaacaa ggttttcatc ggcttcggcg ggtcggagtt cgcggcgcgc ggccgcgtcg
      721 gcgcgttcaa cctcgctgac ggcagcgagg cctgggtctg ccacaccacg gggccggata
      781 aggacgtgtg tctcaccccg gagaccaaca aggccaatcc ccactacggg accgccggcc
      841 aagacctcgg cgtcaagacc ttcccaaacg aggactggaa gatcggcggc gggaccgcct
      901 ggggttggta ctcgtacgac ccggacacga agttggtcta ctactcgacc gcgaaccccg
      961 gcctgtggtc gccccagtat cgctgcccgg acaagaccca cgaggagtgc aacgccgtcg
     1021 acccctccct ggacgggcag gtcggccgct tcgacaacaa atggacgctc tccatcttcg
     1081 cccgcaaaat cgagaacggc gaggccgtct gggtgtatca gatcaccccc ttcgaccagt
     1141 gggactacga cggcatcaac gagaacatca tcacggaaat ggaggtcgat ggcaagaagc
     1201 gaaaggtcct cagccacttc gatcgcaacg gcttcgccta tgtttgggac gccgcggacg
     1261 gcaccgtcct gcgcgccacc aagtatgtga cgaccgactg ggccgagaag atcgatctca
     1321 agacgggacg acccatcaag gtccgggatc attcgccgct cgaggtcggc cgcaatgtca
     1381 gcgcctgccc cagcgcgatg ggcggcaagg accagcagcc ggccagcgtt gacccaaagg
     1441 agccgaacct cttctacgtc ccgaccaaca actggtgcat ggagctggag ccgcaggagc
     1501 gtacgcatac gaaccaaggc acggtctacg tcttcgccaa cgtgtatatg tacccggaga
     1561 agcagggcgt taccgggaag atcaagaagt acgacgtcct gacgggcaag accctctggg
     1621 aaatccccga ccccttcccg aactggtcgg gcaccatgaa caccgacggc gggctgatgt
     1681 tctacggctc gctcggcggg gacttccgcg cggtcgatcg ggataccggc aagatcctct
     1741 ggagcaggaa gctggggtcg ggcatcatcg ggaaccccat taccttcaag acgggcggga
     1801 agcagtacgt cggggtctac agcggcatcg gcggctggat cgggctgccg gtgaccgccg
     1861 ggctggacct caatgacaag ttcggcgcga taggcgccac cgcgatgacc aaggccgccg
     1921 gcctccagca cgtcccgcag ggtggcaccc tgaacgtctt ccgggtctac gacgccgaaa
     1981 acctctattt tcagggacat catcaccatc accatcacca ctgacagct
//
