LOCUS       sequence-306581-        2038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018387
FEATURES             Location/Qualifiers
     source          1..2038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1986..2006
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2007..2030
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgaagttcga gaagctgggc ttgggtatcg gtctcacggc cgcgctgctc ctcgccccgg
      121 tgtccgtgat ggcggaccag tccgcgtccg ccgaaatcga cgagcgcgcc aaggaccccg
      181 acctgtggcc ggcccccggc cgcgataacc agctcacgcg gcactccccg ctcaaggaca
      241 tcaacaccga aaacgtgggc aagctgggca tgatctgggc ccagtccatg aactcgctcc
      301 gcggccaaga ggggcagccc atcgtgatca agctcgagga tggaaccccg atgatgtact
      361 tcgtctcggg ctgcccgtcg atgcccctgt gcaatgtcgt gcaagccctg gatctgacag
      421 accccgataa cccgaagcag atctggaact acgtcaagaa gacggaccgg gacgagtccg
      481 ccattccgcg cgcctgctgc gacaccgtga accgcggcct gaattacgct aagggtaagg
      541 tggtcttcca caccctcgac ggcttcgtga tcgcgctgga cggcaagacc ggcaaggaaa
      601 tctggaccgt gaagcacgcc taccccgagc acggcgagac ccacacgggc ccgaccatga
      661 tcgccgagaa cctcgtcctc gcaggcttcg gcggtgacga attcgccgca cgcggccgca
      721 tcacggccta caacatcgag gacggcagcg aagtgtggtc ctgccatagc accggctcgg
      781 ataaggatgt gtgcctcacc gccgacacca acaaggccaa cccccattac ggctcgtacg
      841 gcaaggacct cggcgtcgtg acctacccga aggacgagtg gaagatcggc ggcggttccc
      901 cgtgggcctg gttctcttac gatccggagc tcaagatgat ctactactcc accggaaacc
      961 caggccactg gtcgccgggc ctgcgatgcg gactggaagg cgaagaccac acccacgaga
     1021 agtgcaacga gttcgacccg gacttgacct ccggcaaatt cgacaacaag tggtccatga
     1081 cgatcttcgg gcggaaggtg gacaccggcg aggctgtctt cgcctaccag atgaccccct
     1141 tcgatcagtg ggactacgac ggagtcaacg agaacatcct tgtcaacatg gaggtcgacg
     1201 gcaaggaccg caaaaccctc gtccacttcg atcgcaatgg cttcgcgtac gtgttggacc
     1261 gggtggacgg caccttgctg cgcgcgaaca agttcgtcac ggtcgattgg gcggagaagg
     1321 tggacctcaa gaccggccgc cctatcaagg tcaaggagca ctcccccttc gatatcggcg
     1381 tcaacacctc cgcctgcccg tccgccatgg gcgggaagga ccagcagccg tgcgccatcg
     1441 acccgaagga aggcaacatc gcgtactgcc ccacgaacaa ttggtgcatg gaggatgagc
     1501 cgcaggagcg cacccacacc cagcagggca ccgtctacgt gttcgccaac gtctacatgt
     1561 acccggagaa gccgggcgtt acaggcaagt tcaagaaatt cgacgtcctc accggaaaga
     1621 ccctgtggga gatccccgac ccgtatccga actggggcgg tgccgcggtg accgacggag
     1681 gcctggtgtt ctacggctca ttgggcgggg acttccgggc ggtcgatagg aagacgggca
     1741 aggtcctgtg gagccggaag ctcggcagcg ggatcatcgg caacccgatc acctacaaga
     1801 tcgatggcaa gcagtatgtg agcgtccatt cgggcatcgg cggctggatt ggccttcctg
     1861 tcacagccgg cctcgatatg agcgacaagt tcggcgccat cggcgcgacc gccatggcga
     1921 aggccaccgg cctgtcgaat atcccgcagg gtggcaccgt caccacgttc cgcctgcttg
     1981 acgccgaaaa cctctatttt cagggacatc atcaccatca ccatcaccac tgacagct
//
