LOCUS       sequence-30660-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Fasano CA, Dimos JT, Ivanova NB, Lowry N, Lemischka IR, Temple S
  TITLE     shRNA knockdown of Bmi-1 reveals a critical role for p21-Rb pathway 
            in NSC self-renewal during development.
  JOURNAL   Cell Stem Cell. 2007 Jun 7. 1(1):87-99.
  PUBMED    18371338
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2007 Jun 7. 1(1):87-99."
COMMENT     Sequence Label: Bmi1 shRNA1
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    181..298
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(596..640)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tttggattgg gatcttataa gttctgtatg agaccactct ttcccgagat aataagcttg
       61 tctattcaag agatagacaa gcttattatc tcttttttgt ctagagatct aagggcgaat
      121 tctttcggat cctttcttaa ttaaccaaac tggatctctg ctgtccctgt aataaacccg
      181 aaaattttga atttttgtaa tttgtttttg taattcttta gtttgtatgt ctgttgctat
      241 tatgtctact attctttccc ctgcactgta ccccccaatc cccccttttc ttttaaaatt
      301 gtggatgaat actgccattt gtctgcagaa ttggcgcacg cagtgccgat ccgttcacta
      361 atcgaatgga tctgtctctg tctctctctc caccttcttc ttctattcct tcgggcctgt
      421 cgggtcccct cggggttggg aggtgggtct gaaacgataa tggtgaatat ccctgcctaa
      481 ctctattcac tatagaaagt acagcaaaaa ctattcttaa acctaccaag cctcctacta
      541 tcattatgaa taattttata taccacagcc aatttgttat gttaaaccaa ttccacaaac
      601 ttgcccattt atctaattcc aataattctt gttcattctt ttcttgctgg ttttgcgatt
      661 cttcaattaa ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc cactccatcc
      721 aggtcgtgtg attccaaatc tgttccagag atttattact ccaactagca ttccaaggca
      781 cagcagtggt gcaaatgagt tttccagagc aacccccaaa tccccaggag ctgttgatcc
      841 tttaggtatc tttccacagc caggattctt gcctggagct gcttgatgcc cagactgtga
      901 gttgca
//
