LOCUS       sequence-30661-e        1024 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Fasano CA, Dimos JT, Ivanova NB, Lowry N, Lemischka IR, Temple S
  TITLE     shRNA knockdown of Bmi-1 reveals a critical role for p21-Rb pathway 
            in NSC self-renewal during development.
  JOURNAL   Cell Stem Cell. 2007 Jun 7. 1(1):87-99.
  PUBMED    18371338
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2007 Jun 7. 1(1):87-99."
COMMENT     Sequence Label: Bmi1 shRNA1
FEATURES             Location/Qualifiers
     source          1..1024
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(134..153)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 gggagctctg cctgctacca tggtggcgac cggtactagt ctaacactga aaattacata
       61 ttgacccaaa tgattacatt tcaaaaggtg cctaaaaaac ttcacaaaac acactcgcca
      121 accccgagcg catagttcaa aaccggagct tcagctactt aagaagatag gtacataaaa
      181 ccgaccaaag aaactgacgc ctcacttatc cctcccctca ccagaggtcc ggcgcctgtc
      241 gattcaggag agcctaccct aggcccgaac cctgcgtcct gcgacggaga aaagcctacc
      301 gcacacctac cggcaggtgg ccccaccctg cattataagc caacagaacg ggtgacgtca
      361 cgacacgacg agggcgcgcg ctcccaaagg tacgggtgca ctgcccaacg gcaccgccat
      421 aactgccgcc cccgcaacag acgacaaacc gagttctcca gtcagtgaca aacttcacgt
      481 cagggtcccc agatggtgcc ccagcccatc tcacccgaat aagagctttc ccgcattagc
      541 gaaggcctca agaccttggg ttcttgccgc ccaccatgcc ccccaccttg tttcaacgac
      601 ctcacagccc gcctcacaag cgtcttccat tcaagactcg ggaacagccg ccattttgct
      661 gcgctccccc caacccccag ttcagggcaa ccttgctcgc ggacccagac tacagccctt
      721 ggcggtctct ccacacgctt ccgtcccacc gagcggcccg gcggccacga aagccccggc
      781 cagcccagca gcccgctact caccaagtga cgatcacagc gatccacaaa caagaaccgc
      841 gacccaaatc ccggctgcga cggaactagc tgtgccacac ccggcgcgtc cttatataat
      901 catcggcgtt caccgcccca cggagatccc ttcgcanaat cgccgagaag ggactacttt
      961 ttctcgcctg ttccgctctc tggaaaaaaa ccagtgccct anagtcaccn aagttccggt
     1021 ccta
//
