LOCUS       sequence-30663-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Fasano CA, Dimos JT, Ivanova NB, Lowry N, Lemischka IR, Temple S
  TITLE     shRNA knockdown of Bmi-1 reveals a critical role for p21-Rb pathway 
            in NSC self-renewal during development.
  JOURNAL   Cell Stem Cell. 2007 Jun 7. 1(1):87-99.
  PUBMED    18371338
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2007 Jun 7. 1(1):87-99."
COMMENT     Sequence Label: Bmi1-overexpression
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(22..30,31..33,34..608)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(195..214)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     424..441
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 gctctcgccc ttgctcacca tggttgtggc catattatca tcgtgttttt caaaggaaaa
       61 ccacgtcccc gtggttcggg gggcctagac gtttttttaa cctcgactaa acacatgtaa
      121 agcatgtgta ccgaggcccc agatcagatc ccatacaatg gggtaccttc tgggcatcct
      181 tcagcccctt gttgaatacg cttgaggaga gccatttgac tctttccaca actatccaac
      241 tcacaacgtg gcactggggt tgtgccgcct ttgcaggtgt atcttataca cgtggctttt
      301 ggccgcagag gcacctgtcg ccaggtgggg ggttccgctg cctgcaaagg gtcgctacag
      361 acgttgtttg tcttcaagaa gcttccagag gaactgcttc cttcacgaca ttcaacagac
      421 cttgcattcc tttggcgaga ggggaaagac ccctaggaat gctcgtcaag aagacagggc
      481 caggtttccg ggccctcaca ttgccaaaag acggcaatat ggtggaaaat aacatataga
      541 caaacgcaca ccggccttat tccaagcggc ttcggccagt aacgttaggg gggggggagg
      601 gagaggggcg gatccctatg aagtatcaca tctagtccat catttatggc caaatttaaa
      661 gtcactacaa aaggaagcaa actggacgac agtcacatgt attaagcatc tataaagctg
      721 taatgaatga aggtaactaa atcagggggt tgaaaaatct tttccttaac agtcctaacc
      781 agatgaagtt gctgatgacc catttagtga tgattttcga ggtctactgg caaaggaaga
      841 ttggtggtta gcactggggc tgttgctggt tccattcatg gtactggaaa tgtgagggaa
      901 ctgtgggtga
//
