LOCUS       sequence-306702-        7461 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Recombinant AAV2 rep/cap plasmid modified to include an 18 amino 
            acid peptide at cap position 586..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7461)
  AUTHORS   Tong JG, Evans AC, Ho ML, Guenther CM, Brun MJ, Judd J, Wu E, Suh J
  TITLE     Reducing off target viral delivery in ovarian cancer gene therapy 
            using a protease-activated AAV2 vector platform.
  JOURNAL   J Control Release. 2019 Jun 25. pii: S0168-3659(19)30367-0. doi: 
            10.1016/j.jconrel.2019.06.034.
  PUBMED    31252037
REFERENCE   2  (bases 1 to 7461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Control 
            Release. 2019 Jun 25. pii: S0168-3659(19)30367-0. doi: 
            10.1016/j.jconrel.2019.06.034."
COMMENT     Sequence Label: pProvector_AAV2_586_PLGLAR
FEATURES             Location/Qualifiers
     source          1..7461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     471..490
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     704..726
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     718..735
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     719..735
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     745..764
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        745..763
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     788..804
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     789..805
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(839..855)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(839..855)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             4983..4994
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(5273..5293)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5273..5291)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5312..5328)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5312..5328)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5325..5347)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5336..5352
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5360..5366,5367..5384,5385..5390))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5405..5426
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5543..5560)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5714..6302)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5794..5813)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6473..7264,7265..7333))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7096..7115
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7334..7438)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc agcgaacgtg gcgagagctt gacggggaaa
      361 gccagcgaac gacggggaaa gccagcgaac gtggcgagac ttgacgggga aagccaggaa
      421 cgtggcgaga aagcttgacg gggaaagcca gcgaacgtgg cgagaaagga agggaagaaa
      481 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
      541 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc
      601 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
      661 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
      721 aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt gggtaccggg
      781 ccccccctcg aggtcgacgg tatcgataag cttgatatcg aattcctgca gcccggggga
      841 tccactagtt ctagagtcct gtattagagg tcacgtgagt gttttgcgac attttgcgac
      901 accatgtggt cacgctgggt atttaagccc gagtgagcac gcagggtctc cattttgaag
      961 cgggaggttt gaacgcgcag ccgccatgcc ggggttttac gagattgtga ttaaggtccc
     1021 cagcgacctt gacgagcatc tgcccggcat ttctgacagc tttgtgaact gggtggccga
     1081 gaaggaatgg gagttgccgc cagattctga catggatctg aatctgattg agcaggcacc
     1141 cctgaccgtg gccgagaagc tgcagcgcga ctttctgacg gaatggcgtc gtgtgagtaa
     1201 ggccccggag gcccttttct ttgtgcaatt tgagaaggga gagagctact tccacatgca
     1261 cgtgctcgtg gaaaccaccg gggtgaaatc tatggttttg ggacgtttcc tgagtcagat
     1321 tcgcgaaaaa ctgattcaga gaatttaccg cgggatcgag ccgactttgc caaactggtt
     1381 cgcggtcaca aagaccagaa atggtgccgg aggcgggaac aaggtggtgg atgagtgcta
     1441 catccccaat tacttgctcc ccaaaaccca gcctgagctc cagtgggcgt ggactaatat
     1501 ggaacagtat ttaagcgcct gtttgaatct cacggagcgt aaacggttgg tggcgcagca
     1561 tctgacgcac gtgtcgcaga cgcaggagca gaacaaagag aatcagaatc ccaattctga
     1621 tgcgccggtg atcagatcaa aaacttcagc caggtacatg gagctggtcg ggtggctcgt
     1681 ggacaagggg attacctcgg agaagcagtg gatacaggag gaccaggcct catacatctc
     1741 cttcaatgcg gcctccaact cgcggtccca aatcaaggct gccttggaca atgcgggaaa
     1801 gattatgagc ctgactaaaa ccgcccccga ctacctggtg ggccagcagc ccgtggagga
     1861 catttccagc aatcggattt ataaaatttt ggaactaaac gggtacgatc cccaatatgc
     1921 ggcttccgtc tttctgggat gggccacgaa aaagttcggc aagaggaaca ccatctggct
     1981 gtttgggcct gcaactaccg ggaagaccaa catcgcggag gccatagccc acactgtgcc
     2041 cttctacggg tgcgtaaact ggaccaatga gaactttccc ttcaacgact gtgtcgacaa
     2101 gatggtgatc tggtgggagg aggggaagat gaccgccaag gtcgtggagt cggccaaagc
     2161 cattctcgga ggaagcaagg tgcgcgtgga ccagaaatgc aagtcctcgg cccagataga
     2221 cccgactccc gtgatcgtca cctccaacac caacatgtgc gccgtgattg acgggaactc
     2281 aacgaccttc gaacaccagc agccgttgca agaccggatg ttcaaatttg aactcacccg
     2341 ccgtctggat catgactttg ggaaggtcac caagcaggaa gtcaaagact ttttccggtg
     2401 ggcaaaggat cacgtggttg aggtggagca tgaattctac gtcaaaaagg gtggagccaa
     2461 gaaaagaccc gcccccagtg acgcagatat aagtgagccc aaacgggtgc gcgagtcagt
     2521 tgcgcagcca tcgacgtcag acgcggaagc ttcgatcaac tacgcagaca ggtaccaaaa
     2581 caaatgttct cgtcacgtgg gcatgaatct gatgctgttt ccctgcagac aatgcgagag
     2641 aatgaatcag aattcaaata tctgcttcac tcacggacag aaagactgtt tagagtgctt
     2701 tcccgtgtca gaatctcaac ccgtttctgt cgtcaaaaag gcgtatcaga aactgtgcta
     2761 cattcatcat atcatgggaa aggtgccaga cgcttgcact gcctgcgatc tggtcaatgt
     2821 ggatttggat gactgcatct ttgaacaata aatgatttaa atcaggtatg gctgccgatg
     2881 gttatcttcc agattggctc gaggacactc tctctgaagg aataagacag tggtggaagc
     2941 tcaaacctgg cccaccacca ccaaagcccg cagagcggca taaggacgac agcaggggtc
     3001 ttgtgcttcc tgggtacaag tacctcggac ccttcaacgg actcgacaag ggagagccgg
     3061 tcaacgaggc agacgccgcg gccctcgagc acgacaaagc ctacgaccgg cagctcgaca
     3121 gcggagacaa cccgtacctc aagtacaacc acgccgacgc ggagtttcag gagcgcctta
     3181 aagaagatac gtcttttggg ggcaacctcg gacgagcagt cttccaggcg aaaaagaggg
     3241 ttcttgaacc tctgggcctg gttgaggaac ctgttaagac ggctccggga aaaaagaggc
     3301 cggtagagca ctctcctgtg gagccagact cctcctcggg aaccggaaag gcgggccagc
     3361 agcctgcaag aaaaagattg aattttggtc agactggaga cgcagactca gtacctgacc
     3421 cccagcctct cggacagcca ccagcagccc cctctggtct gggaactaat accatggcta
     3481 caggcagtgg cgcaccaatg gcagacaata acgagggtgc cgacggagtg ggtaattcct
     3541 cgggaaattg gcattgcgat tccacatgga tgggcgacag agtcatcacc accagcaccc
     3601 gaacctgggc cctgcccacc tacaacaacc acctctacaa acaaatttcc agccaatcag
     3661 gagcctcgaa cgacaatcac tactttggct acagcacccc ttgggggtat tttgacttca
     3721 acagattcca ctgccacttt tcaccacgtg actggcaaag actcatcaac aacaactggg
     3781 gattccgacc caagagactc aacttcaagc tctttaacat tcaagtcaaa gaggtcacgc
     3841 agaatgacgg tacgacgacg attgccaata accttaccag cacggttcag gtgtttactg
     3901 actcggagta ccagctcccg tacgtcctcg gctcggcgca tcaaggatgc ctcccgccgt
     3961 tcccagcaga cgtcttcatg gtgccacagt atggatacct caccctgaac aacgggagtc
     4021 aggcagtagg acgctcttca ttttactgcc tggagtactt tccttctcag atgctgcgta
     4081 ccggaaacaa ctttaccttc agctacactt ttgaggacgt tcctttccac agcagctacg
     4141 ctcacagcca gagtctggac cgtctcatga atcctctcat cgaccagtac ctgtattact
     4201 tgagcagaac aaacactcca agtggaacca ccacgcagtc aaggcttcag ttttctcagg
     4261 ccggagcgag tgacattcgg gaccagtcta ggaactggct tcctggaccc tgttaccgcc
     4321 agcagcgagt atcaaagaca tctgcggata acaacaacag tgaatactcg tggactggag
     4381 ctaccaagta ccacctcaat ggcagagact ctctggtgaa tccgggcccg gctatggcaa
     4441 gccacaagga cgatgaagaa aagttttttc ctcagagcgg ggttctcatc tttgggaagc
     4501 aaggctcaga gaaaacaaat gtggacattg aaaaggtcat gattacagac gaagaggaaa
     4561 tcaggacaac caatcccgtg gctacggagc agtatggttc tgtatctacc aacctccaga
     4621 gaggagccgg ccctctggga ctcgccagag gtgatgatga cgacggacca ctgggactgg
     4681 cacgtggcgc caacagacaa gcagctaccg cagatgtcaa cacacaaggc gttcttccag
     4741 gcatggtctg gcaggacaga gatgtgtacc ttcaggggcc catctgggca aagattccac
     4801 acacggacgg acattttcac ccctctcccc tcatgggtgg attcggactt aaacaccctc
     4861 ctccacagat tctcatcaag aacaccccgg tgcctgcgaa tccttcgacc accttcagtg
     4921 cggcaaagtt tgcttccttc atcacacagt actccacggg acaggtcagc gtggagatcg
     4981 agtgggagct gcagaaggaa aacagcaaac gctggaatcc cgaaattcag tacacttcca
     5041 actacaacaa gtctgttaat gtggacttta ctgtggacac taatggcgtg tattcagagc
     5101 ctcgccccat tggcaccaga tacctgactc gtaatctgta attggcaggt cttgttaatc
     5161 aataaaccgt ttaattcgtt tcagttgaac tttggtctct gcgtatttct ttcttatcta
     5221 gtttccatgc tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt
     5281 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     5341 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     5401 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     5461 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     5521 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     5581 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     5641 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     5701 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     5761 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     5821 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     5881 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     5941 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6001 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6061 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6121 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     6181 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6241 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     6301 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     6361 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     6421 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     6481 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     6541 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     6601 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     6661 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     6721 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     6781 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     6841 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     6901 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     6961 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7021 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7081 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7141 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     7201 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     7261 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     7321 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     7381 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     7441 catttccccg aaaagtgcca c
//
