LOCUS       sequence-30675-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Pera EM, Kim JI, Martinez SL, Brechner M, Li SY, Wessely O, De 
            Robertis EM
  TITLE     Isthmin is a novel secreted protein expressed as part of the Fgf-8 
            synexpression group in the Xenopus midbrain-hindbrain organizer.
  JOURNAL   Mech Dev. 2002 Aug . 116(1-2):169-72.
  PUBMED    12128218
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mech Dev. 
            2002 Aug . 116(1-2):169-72."
COMMENT     Sequence Label: pCS2 xIGF2
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    624..758
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(648..667)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     702..721
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(871..891)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(871..889)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(910..926)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(910..926)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(923..945)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tccatcgatt cgaattcgag tacacagctg ccaaggatgt cagtcatgag gcatctattc
       61 ctcctctcta tcacctttct ggtatacacc ctagactctg ctaaagccta cagaccaacg
      121 gagaccctgt gcggcgggga gctagtggac accttgcagt ttgtttgcgg agacaggggc
      181 ttctatttca gcacgaataa tggccgctcc aaccgtaggt ccaacagggg gattgtggaa
      241 gaatgttgct tccggagctg tgatctggaa ctgttggaaa catactgtgc aaagccatct
      301 aagaatgaga gggatgtctc cactgcaccc gccacagcaa taccaccaat gaacaagcag
      361 gacctgtatc acaaacatca ccacacaaaa agctccaagt atgatatctg gcaaaggaag
      421 tccatccatc ggctcagaag aggtgtccct gctattgtac gtgctaggca gtatcggttg
      481 ctcatgcaga aggctgaaga atcagagcag gcactattgc atcggcccct taccacctta
      541 cccataacgc ggcctctcca tctgcagcaa acctcagaac cttcccacaa ttgatctaga
      601 actatagtga gtcgtattac gtagatccag acatgataag atacattgat gagtttggac
      661 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
      721 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      781 ttatgtttca ggttcagggg gaggtgtggg aggtttttta attcgcggcc gcggcgccaa
      841 tgcattgggc ccggtaccca gcttttgttc cctttagtga gggttaattg cgcgcttggc
      901 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcac
//
